| PELCOVÁ, Marta, Roman ŘEMÍNEK, Friederike A SANDBAUMHÜTER, Richard A MOSHER, Zdeněk GLATZ and Wolfgang THORMANN. Simulation and experimental study of enzyme and reactant mixing in capillary electrophoresis based on-line methods. Journal of Chromatography A. Holandsko: Elsevier, 2016, vol. 1471, November, p. 192-200. ISSN 0021-9673. Available from: https://dx.doi.org/10.1016/j.chroma.2016.10.002. |
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@article{1355796,
author = {Pelcová, Marta and Řemínek, Roman and Sandbaumhüter, Friederike A and Mosher, Richard A and Glatz, Zdeněk and Thormann, Wolfgang},
article_location = {Holandsko},
article_number = {November},
doi = {http://dx.doi.org/10.1016/j.chroma.2016.10.002},
keywords = {capillary electrophoresis; enzyme reaction; electrophoretically mediated microanalysis; diffusion; simulation; Taylor-Aris dispersion},
language = {eng},
issn = {0021-9673},
journal = {Journal of Chromatography A},
title = {Simulation and experimental study of enzyme and reactant mixing in capillary electrophoresis based on-line methods},
volume = {1471},
year = {2016}
}
TY - JOUR
ID - 1355796
AU - Pelcová, Marta - Řemínek, Roman - Sandbaumhüter, Friederike A - Mosher, Richard A - Glatz, Zdeněk - Thormann, Wolfgang
PY - 2016
TI - Simulation and experimental study of enzyme and reactant mixing in capillary electrophoresis based on-line methods
JF - Journal of Chromatography A
VL - 1471
IS - November
SP - 192-200
EP - 192-200
PB - Elsevier
SN - 00219673
KW - capillary electrophoresis
KW - enzyme reaction
KW - electrophoretically mediated microanalysis
KW - diffusion
KW - simulation
KW - Taylor-Aris dispersion
N2 - The establishment of an efficient reaction mixture represents a crucial part of capillary electrophoresis based on-line enzymatic assays. For ketamine N-demethylation to norketamine mediated by the cytochrome P450 3A4 enzyme, mixing of enzyme and reactants in the incubation buffer at physiological pH was studied by computer simulation. A dynamic electrophoretic simulator that encompasses Taylor-Aris diffusivity which accounts for dispersion due to the parabolic flow profile associated with pressure driven flow was utilized. The simulator in the diffusion mode was used to predict transverse diffusional reactant mixing occurring during hydrodynamic plug injection of configurations featuring four and seven plugs. The same simulator in the electrophoretic mode was applied to study electrophoretic reactant mixing caused by voltage application in absence of buffer flow. Resulting conclusions were experimentally verified with enantioselective analysis of norketamine in a background electrolyte at low pH. Furthermore, simulations visualize buffer changes that occur upon power application between incubation buffer and background electrolyte and have an influence on the reaction mixture.
ER -
PELCOVÁ, Marta, Roman ŘEMÍNEK, Friederike A SANDBAUMHÜTER, Richard A MOSHER, Zdeněk GLATZ and Wolfgang THORMANN. Simulation and experimental study of enzyme and reactant mixing in capillary electrophoresis based on-line methods. \textit{Journal of Chromatography A}. Holandsko: Elsevier, 2016, vol.~1471, November, p.~192-200. ISSN~0021-9673. Available from: https://dx.doi.org/10.1016/j.chroma.2016.10.002.
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