Detailed Information on Publication Record

GOÜY DE BELLOCQ, Joëlle, Jana TĚŠÍKOVÁ, Yonas MEHERETU, Dagmar ČÍŽKOVÁ, Anna BRYJOVÁ, Herwig LEIRS and Josef BRYJA. Complete genome characterisation and phylogenetic position of Tigray hantavirus from the Ethiopian white-footed mouse, Stenocephalemys albipes. Infection, Genetics and Evolution. Amsterdam: Elsevier Science, 2016, vol. 45, November, p. 242-245. ISSN 1567-1348. Available from: https://dx.doi.org/10.1016/j.meegid.2016.09.009.

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Basic information
Original name	Complete genome characterisation and phylogenetic position of Tigray hantavirus from the Ethiopian white-footed mouse, Stenocephalemys albipes
Authors	GOÜY DE BELLOCQ, Joëlle (250 France), Jana TĚŠÍKOVÁ (203 Czech Republic, belonging to the institution), Yonas MEHERETU (231 Ethiopia), Dagmar ČÍŽKOVÁ (203 Czech Republic), Anna BRYJOVÁ (203 Czech Republic), Herwig LEIRS (56 Belgium) and Josef BRYJA (203 Czech Republic, guarantor, belonging to the institution).
Edition	Infection, Genetics and Evolution, Amsterdam, Elsevier Science, 2016, 1567-1348.

Other information
Original language	English
Type of outcome	Article in a journal
Field of Study	10700 1.7 Other natural sciences
Country of publisher	Netherlands
Confidentiality degree	is not subject to a state or trade secret
Impact factor	Impact factor: 2.885
RIV identification code	RIV/00216224:14310/16:00095886
Organization unit	Faculty of Science
Doi	http://dx.doi.org/10.1016/j.meegid.2016.09.009
UT WoS	000388574400032
Keywords in English	Hantavirus; Murinae; Ethiopia; High throughput sequencing; Genomics
Tags	NZ, rivok
Tags	International impact, Reviewed
Changed by	Changed by: Ing. Nicole Zrilić, učo 240776. Changed: 13/4/2018 12:59.

Abstract
Hantaviruses, well-known human pathogens, have only recently been identified on the African continent. Tigray virus (TIGV) was found in Ethiopia in 2012 in a Murinae species, Stenocephalemys albipes, but the genetic data obtained at that time were too limited to correctly assess its phylogenetic position within the hantavirus tree. We used high throughput sequencing to determine the complete genome of TIGV, which showed a typical hantavirus organisation. The large (L), medium (M), and small (S) genome segments were found to be 6532, 3594 and 1908 nucleotides long, respectively, and the 5' and 3' termini for all three segments were predicted to form the panhandle-like structure typical for bunyaviruses. Nucleotide-based phylogenetic analyses revealed that all three coding segments cluster in the phylogroup III sensu Guo et al. (2013). However, while TIGV S segment is basal to the Murinae-associated hantaviruses, the M and L segments are basal to the Soricomorpha-associated hantaviruses. TIGV is the first Murinae-borne hantavirus showing this inconsistent segmental clustering in the hantavirus phylogenetic tree. We finally propose non-exclusive scenarios that could explain the original phylogenetic position of TIGV. (C) 2016 Elsevier B.V. All rights reserved.

Abstract

Hantaviruses, well-known human pathogens, have only recently been identified on the African continent. Tigray virus (TIGV) was found in Ethiopia in 2012 in a Murinae species, Stenocephalemys albipes, but the genetic data obtained at that time were too limited to correctly assess its phylogenetic position within the hantavirus tree. We used high throughput sequencing to determine the complete genome of TIGV, which showed a typical hantavirus organisation. The large (L), medium (M), and small (S) genome segments were found to be 6532, 3594 and 1908 nucleotides long, respectively, and the 5' and 3' termini for all three segments were predicted to form the panhandle-like structure typical for bunyaviruses. Nucleotide-based phylogenetic analyses revealed that all three coding segments cluster in the phylogroup III sensu Guo et al. (2013). However, while TIGV S segment is basal to the Murinae-associated hantaviruses, the M and L segments are basal to the Soricomorpha-associated hantaviruses. TIGV is the first Murinae-borne hantavirus showing this inconsistent segmental clustering in the hantavirus phylogenetic tree. We finally propose non-exclusive scenarios that could explain the original phylogenetic position of TIGV. (C) 2016 Elsevier B.V. All rights reserved.