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@article{1355887,
author = {Kolesár, Peter and Altmannová, Veronika and Silva, Sonia and Lisby, Michael and Krejčí, Lumír},
article_location = {Bethesda},
article_number = {14},
doi = {http://dx.doi.org/10.1074/jbc.M115.685891},
keywords = {DNA repair; Homologous recombination; Possible mechanisms; Post-translational modifications; Proliferating cell nuclear antigens; Recombination factors; Small ubiquitin-like modifiers; Sumoylation},
language = {eng},
issn = {0021-9258},
journal = {Journal of Biological Chemistry},
title = {Pro-recombination Role of Srs2 Protein Requires SUMO (Small ubiquitin-like Modifier) but Is Independent of PCNA (Proliferating Cell Nuclear Antigen) Interaction},
volume = {291},
year = {2016}
}
TY - JOUR
ID - 1355887
AU - Kolesár, Peter - Altmannová, Veronika - Silva, Sonia - Lisby, Michael - Krejčí, Lumír
PY - 2016
TI - Pro-recombination Role of Srs2 Protein Requires SUMO (Small ubiquitin-like Modifier) but Is Independent of PCNA (Proliferating Cell Nuclear Antigen) Interaction
JF - Journal of Biological Chemistry
VL - 291
IS - 14
SP - 7594-7607
EP - 7594-7607
PB - American Society for Biochemistry and Molecular Biology Inc.
SN - 00219258
KW - DNA repair
KW - Homologous recombination
KW - Possible mechanisms
KW - Post-translational modifications
KW - Proliferating cell nuclear antigens
KW - Recombination factors
KW - Small ubiquitin-like modifiers
KW - Sumoylation
N2 - Srs2 plays many roles in DNA repair, the proper regulation and coordination of which is essential. Post-translational modification by small ubiquitin-like modifier (SUMO) is one such possible mechanism. Here, we investigate the role of SUMO in Srs2 regulation and show that the SUMO-interacting motif (SIM) of Srs2 is important for the interaction with several recombination factors. Lack of SIM, but not proliferating cell nuclear antigen (PCNA)-interacting motif (PIM), leads to increased cell death under circumstances requiring homologous recombination for DNA repair. Simultaneous mutation of SIM in a srs2 deltaPIM strain leads to a decrease in recombination, indicating a pro-recombination role of SUMO. Thus SIM has an ambivalent function in Srs2 regulation; it not only mediates interaction with SUMO-PCNA to promote the anti-recombination function but it also plays a PCNA-independent pro-recombination role, probably by stimulating the formation of recombination complexes. The fact that deletion of PIM suppresses the phenotypes of Srs2 lacking SIM suggests that proper balance between the anti-recombination PCNA-bound and pro-recombination pools of Srs2 is crucial. Notably, sumoylation of Srs2 itself specifically stimulates recombination at the rDNA locus.
ER -
KOLESÁR, Peter, Veronika ALTMANNOVÁ, Sonia SILVA, Michael LISBY and Lumír KREJČÍ. Pro-recombination Role of Srs2 Protein Requires SUMO (Small ubiquitin-like Modifier) but Is Independent of PCNA (Proliferating Cell Nuclear Antigen) Interaction. \textit{Journal of Biological Chemistry}. Bethesda: American Society for Biochemistry and Molecular Biology Inc., 2016, vol.~291, No~14, p.~7594-7607. ISSN~0021-9258. Available from: https://dx.doi.org/10.1074/jbc.M115.685891.
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