Detailed Information on Publication Record
2016
Cystatins of the parasitic nematodes Trichinella spiralis and T. pseudospiralis: xMAP technology in serodiagnostics.
ŠKORPÍKOVÁ, Lucie, Martin KAŠNÝ, Michal SLANÝ, Petr KRÁLÍK, Milan GELNAR et. al.Basic information
Original name
Cystatins of the parasitic nematodes Trichinella spiralis and T. pseudospiralis: xMAP technology in serodiagnostics.
Name in Czech
Cystatiny parazitických hlístic rodu Trichinella spiralis a T. pseudospiralis: xMAP technologie v serodiagnostice.
Name (in English)
Cystatins of the parasitic nematodes Trichinella spiralis and T. pseudospiralis: xMAP technology in serodiagnostics.
Authors
ŠKORPÍKOVÁ, Lucie (203 Czech Republic, belonging to the institution), Martin KAŠNÝ (203 Czech Republic), Michal SLANÝ (203 Czech Republic), Petr KRÁLÍK (203 Czech Republic), Milan GELNAR (203 Czech Republic) and Jana ILGOVÁ (703 Slovakia)
Edition
12. České a slovenské parazitologické dny, 2016
Other information
Language
Czech
Type of outcome
Prezentace na konferencích
Field of Study
10600 1.6 Biological sciences
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/16:00091092
Organization unit
Faculty of Science
Keywords (in Czech)
cystatiny; Trichinella, serodiagnostika; potravinoví parazité
Keywords in English
cystatins; Trichinella; serodiagnstics; food-borne parasites
Změněno: 24/3/2017 15:29, RNDr. Martin Kašný, Ph.D.
V originále
Nematodes of the genus Trichinella are clinically important parasites invading cells of the small intestine and subsequently skeletal muscles. Their global distribution is corresponding to the wide range of their hosts involving birds, reptiles and mammals. Seven (T1, T2, T3, T4, T5, T6 a T10) of twelve genotypes of Trichinella spp. are zoonoses causing the trichinellosis - a serious and sometimes fatal human disease. During the life cycle, the individuals of this nematode parasite release variety of functional molecules, which are mainly present in excretory-secretory (E-S) products. These molecules are essential for many biological processes like development, digestion, migration, and also in manipulation of the host immunne system. The most dominant components of the E-S products are cystatins – inhibitors of cysteine peptidases of C1 and rarely of C13 family. Our work is focused on the characterization of „multi cystatin-like domain“ (mcd-1) genes and proteins of Trichinella spiralis and T. pseudospiralis. The complete sequences of genes were obtained and recombinant analogs of MCD-1 proteins were prepared. The recombinant MCD-1 molecules are used also in regard to develop the reliable molecular method useful for serological quantitative and qualitative diagnosis of parasitic nematodes from the genus Trichinella. We employed the xMAP technology - novel diagnostic platform combining immunoassay approach and magnetic microspheres.
In English
Nematodes of the genus Trichinella are clinically important parasites invading cells of the small intestine and subsequently skeletal muscles. Their global distribution is corresponding to the wide range of their hosts involving birds, reptiles and mammals. Seven (T1, T2, T3, T4, T5, T6 a T10) of twelve genotypes of Trichinella spp. are zoonoses causing the trichinellosis - a serious and sometimes fatal human disease. During the life cycle, the individuals of this nematode parasite release variety of functional molecules, which are mainly present in excretory-secretory (E-S) products. These molecules are essential for many biological processes like development, digestion, migration, and also in manipulation of the host immunne system. The most dominant components of the E-S products are cystatins – inhibitors of cysteine peptidases of C1 and rarely of C13 family. Our work is focused on the characterization of „multi cystatin-like domain“ (mcd-1) genes and proteins of Trichinella spiralis and T. pseudospiralis. The complete sequences of genes were obtained and recombinant analogs of MCD-1 proteins were prepared. The recombinant MCD-1 molecules are used also in regard to develop the reliable molecular method useful for serological quantitative and qualitative diagnosis of parasitic nematodes from the genus Trichinella. We employed the xMAP technology - novel diagnostic platform combining immunoassay approach and magnetic microspheres.
Links
| MUNI/A/1325/2015, interní kód MU |
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