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@proceedings{1356195,
author = {Škorpíková, Lucie and Gelnar, Milan and Slaný, Michal and Kašný, Martin and Králík, Petr and Ilgová, Jana and Snížková, Karolína},
booktitle = {EMOP XII – the 12th European Multicolloquium of Parasitology},
keywords = {xMAP technology; immunodiagnostics; cystatins; Trichinella spiralis; Trichinella pseudospiralis},
language = {eng},
title = {Cystatins of the parasitic nematodes from the genus Trichinella: serodiagnostic assays based on the xMAP-technology.},
url = {http://congress.utu.fi/emop2016/index.php},
year = {2016}
}
TY - CONF
ID - 1356195
AU - Škorpíková, Lucie - Gelnar, Milan - Slaný, Michal - Kašný, Martin - Králík, Petr - Ilgová, Jana - Snížková, Karolína
PY - 2016
TI - Cystatins of the parasitic nematodes from the genus Trichinella: serodiagnostic assays based on the xMAP-technology.
KW - xMAP technology
KW - immunodiagnostics
KW - cystatins
KW - Trichinella spiralis
KW - Trichinella pseudospiralis
UR - http://congress.utu.fi/emop2016/index.php
N2 - Roundworms of the genus Trichinella are clinically important parasites invading cells of the small intestine and skeletal muscles. They infect a broad range of hosts involving birds, reptiles and mammals. Seven of twelve genotypes of Trichinella spp. are zoonoses causing the trichinellosis - a serious human disease. During the life cycle, these parasites release variety of functional molecules, which are mainly present in excretory-secretory (E-S) products. They are essential for many biological processes like migration, digestion, development, and also in manipulation of the host immunne system. The dominant components of the E-S products are cystatins - reversible inhibitors of cysteine peptidases of C1 (papain-like) family and rarely of C13 (legumain-like) family. All the representatives of cystatin superfamily are evolutionary related and share some sequential and structural identity. Our work is focused on the characterization of "multi cystatin-like domain" (mcd-1) genes and proteins of Trichinella spiralis and T. pseudospiralis. The complete sequences of genes were obtained and recombinant analogs of MCD-1 proteins were prepared in Escherichia coli expression system. Currently, the recombinant MCD-1 molecules are used also in regard to develop the reliable molecular method useful for serological quantitative and qualitative diagnostics of Trichinella. We employed the xMAP technology - novel diagnostic platform based on magnetic microspheres and a signal evaluation by MAGPIX device.
ER -
ŠKORPÍKOVÁ, Lucie, Milan GELNAR, Michal SLANÝ, Martin KAŠNÝ, Petr KRÁLÍK, Jana ILGOVÁ and Karolína SNÍŽKOVÁ. Cystatins of the parasitic nematodes from the genus Trichinella: serodiagnostic assays based on the xMAP-technology. In \textit{EMOP XII – the 12th European Multicolloquium of Parasitology}. 2016.
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