Relation of exposure to amino acids involved in sarcosine
metabolic pathway on behavior of non-tumor and malignant
prostatic cell lines

J 2016

Relation of exposure to amino acids involved in sarcosine metabolic pathway on behavior of non-tumor and malignant prostatic cell lines

HEGER, Zbynek, Jaromír GUMULEC, Natalia CERNEI, Hana POLANSKÁ, Martina RAUDENSKÁ et. al.

Basic information

Original name

Relation of exposure to amino acids involved in sarcosine metabolic pathway on behavior of non-tumor and malignant prostatic cell lines

Authors

HEGER, Zbynek (203 Czech Republic), Jaromír GUMULEC (203 Czech Republic, belonging to the institution), Natalia CERNEI (203 Czech Republic), Hana POLANSKÁ (203 Czech Republic, belonging to the institution), Martina RAUDENSKÁ (203 Czech Republic, belonging to the institution), Michal MASAŘÍK (203 Czech Republic, guarantor, belonging to the institution), Tomas ECKSCHLAGER (203 Czech Republic), Marie STIBOROVA (203 Czech Republic), Vojtech ADAM (203 Czech Republic) and Rene KIZEK (203 Czech Republic)

Edition

Prostate, Hoboken, Wiley-Blackwell, 2016, 0270-4137

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30200 3.2 Clinical medicine

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.820

RIV identification code

RIV/00216224:14110/16:00091191

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1002/pros.23159

UT WoS

000373932700007

Keywords in English

cancer metabolism; dimethylglycine; folate; glycine; sarcosine pathway; prostate cancer

Abstract

V originále

BACKGROUND Sarcosine (N-methylglycine) was previously delineated as a substantial oncometabolite of prostate cancer (PCa) and its metabolism seems to be significantly involved in PCa development and behavior. METHODS We focused on investigation whether the exposure of prostate cells (PNT1A, 22Rv1, and PC-3) to sarcosine-related amino acids (glycine, dimethylglycine, and sarcosine) affects their aggressiveness (cell mobility and division rates, using real-time cell based assay). The effect of supplementation on expression of glycine-N-methyltransferase (GNMT) mRNA was examined using qRT-PCR. Finally, post-treatment amino acids patterns were determined with consequent statistical processing using the Ward's method, factorial ANOVA and principal component analysis (P<0.05). RESULTS The highest migration induced sarcosine and glycine in metastatic PC-3 cells (a decrease in relative free area about 53% and 73%). The highest cell division was achieved after treatment of 22Rv1 and PC-3 cells with sarcosine (time required for division decreased by 65% or 45%, when compared to untreated cells). qRT-PCR revealed also significant effects on expression of GNMT. Finally, amino acid profiling shown specific amino acid patterns for each cell line. In both, treated and untreated PC-3 cells significantly higher levels of serine, glutamic acid, and aspartate, linked with prostate cancer progression were found. CONCLUSIONS Sarcosine-related amino acids can exceptionally affect the behavior of benign and malignant prostate cells.

Citovat

HEGER, Zbynek, Jaromír GUMULEC, Natalia CERNEI, Hana POLANSKÁ, Martina RAUDENSKÁ, Michal MASAŘÍK, Tomas ECKSCHLAGER, Marie STIBOROVA, Vojtech ADAM and Rene KIZEK. Relation of exposure to amino acids involved in sarcosine metabolic pathway on behavior of non-tumor and malignant prostatic cell lines. Prostate. Hoboken: Wiley-Blackwell, 2016, vol. 76, No 7, p. 679-690. ISSN 0270-4137. Available from: https://dx.doi.org/10.1002/pros.23159.

@article{1356683,
   author = {Heger, Zbynek and Gumulec, Jaromír and Cernei, Natalia and Polanská, Hana and Raudenská, Martina and Masařík, Michal and Eckschlager, Tomas and Stiborova, Marie and Adam, Vojtech and Kizek, Rene},
   article_location = {Hoboken},
   article_number = {7},
   doi = {http://dx.doi.org/10.1002/pros.23159},
   keywords = {cancer metabolism; dimethylglycine; folate; glycine; sarcosine pathway; prostate cancer},
   language = {eng},
   issn = {0270-4137},
   journal = {Prostate},
   title = {Relation of exposure to amino acids involved in sarcosine metabolic pathway on behavior of non-tumor and malignant prostatic cell lines},
   volume = {76},
   year = {2016}
}

TY  - JOUR
ID  - 1356683
AU  - Heger, Zbynek - Gumulec, Jaromír - Cernei, Natalia - Polanská, Hana - Raudenská, Martina - Masařík, Michal - Eckschlager, Tomas - Stiborova, Marie - Adam, Vojtech - Kizek, Rene
PY  - 2016
TI  - Relation of exposure to amino acids involved in sarcosine metabolic pathway on behavior of non-tumor and malignant prostatic cell lines
JF  - Prostate
VL  - 76
IS  - 7
SP  - 679-690
EP  - 679-690
PB  - Wiley-Blackwell
SN  - 02704137
KW  - cancer metabolism
KW  - dimethylglycine
KW  - folate
KW  - glycine
KW  - sarcosine pathway
KW  - prostate cancer
N2  - BACKGROUND Sarcosine (N-methylglycine) was previously delineated as a substantial oncometabolite of prostate cancer (PCa) and its metabolism seems to be significantly involved in PCa development and behavior. METHODS We focused on investigation whether the exposure of prostate cells (PNT1A, 22Rv1, and PC-3) to sarcosine-related amino acids (glycine, dimethylglycine, and sarcosine) affects their aggressiveness (cell mobility and division rates, using real-time cell based assay). The effect of supplementation on expression of glycine-N-methyltransferase (GNMT) mRNA was examined using qRT-PCR. Finally, post-treatment amino acids patterns were determined with consequent statistical processing using the Ward's method, factorial ANOVA and principal component analysis (P<0.05). RESULTS The highest migration induced sarcosine and glycine in metastatic PC-3 cells (a decrease in relative free area about 53% and 73%). The highest cell division was achieved after treatment of 22Rv1 and PC-3 cells with sarcosine (time required for division decreased by 65% or 45%, when compared to untreated cells). qRT-PCR revealed also significant effects on expression of GNMT. Finally, amino acid profiling shown specific amino acid patterns for each cell line. In both, treated and untreated PC-3 cells significantly higher levels of serine, glutamic acid, and aspartate, linked with prostate cancer progression were found. CONCLUSIONS Sarcosine-related amino acids can exceptionally affect the behavior of benign and malignant prostate cells.
ER  -

HEGER, Zbynek, Jaromír GUMULEC, Natalia CERNEI, Hana POLANSKÁ, Martina RAUDENSKÁ, Michal MASAŘÍK, Tomas ECKSCHLAGER, Marie STIBOROVA, Vojtech ADAM and Rene KIZEK. Relation of exposure to amino acids involved in sarcosine metabolic pathway on behavior of non-tumor and malignant prostatic cell lines. \textit{Prostate}. Hoboken: Wiley-Blackwell, 2016, vol.~76, No~7, p.~679-690. ISSN~0270-4137. Available from: https://dx.doi.org/10.1002/pros.23159.

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