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@article{1356683,
author = {Heger, Zbynek and Gumulec, Jaromír and Cernei, Natalia and Polanská, Hana and Raudenská, Martina and Masařík, Michal and Eckschlager, Tomas and Stiborova, Marie and Adam, Vojtech and Kizek, Rene},
article_location = {Hoboken},
article_number = {7},
doi = {http://dx.doi.org/10.1002/pros.23159},
keywords = {cancer metabolism; dimethylglycine; folate; glycine; sarcosine pathway; prostate cancer},
language = {eng},
issn = {0270-4137},
journal = {Prostate},
title = {Relation of exposure to amino acids involved in sarcosine metabolic pathway on behavior of non-tumor and malignant prostatic cell lines},
volume = {76},
year = {2016}
}
TY - JOUR
ID - 1356683
AU - Heger, Zbynek - Gumulec, Jaromír - Cernei, Natalia - Polanská, Hana - Raudenská, Martina - Masařík, Michal - Eckschlager, Tomas - Stiborova, Marie - Adam, Vojtech - Kizek, Rene
PY - 2016
TI - Relation of exposure to amino acids involved in sarcosine metabolic pathway on behavior of non-tumor and malignant prostatic cell lines
JF - Prostate
VL - 76
IS - 7
SP - 679-690
EP - 679-690
PB - Wiley-Blackwell
SN - 02704137
KW - cancer metabolism
KW - dimethylglycine
KW - folate
KW - glycine
KW - sarcosine pathway
KW - prostate cancer
N2 - BACKGROUND Sarcosine (N-methylglycine) was previously delineated as a substantial oncometabolite of prostate cancer (PCa) and its metabolism seems to be significantly involved in PCa development and behavior. METHODS We focused on investigation whether the exposure of prostate cells (PNT1A, 22Rv1, and PC-3) to sarcosine-related amino acids (glycine, dimethylglycine, and sarcosine) affects their aggressiveness (cell mobility and division rates, using real-time cell based assay). The effect of supplementation on expression of glycine-N-methyltransferase (GNMT) mRNA was examined using qRT-PCR. Finally, post-treatment amino acids patterns were determined with consequent statistical processing using the Ward's method, factorial ANOVA and principal component analysis (P<0.05). RESULTS The highest migration induced sarcosine and glycine in metastatic PC-3 cells (a decrease in relative free area about 53% and 73%). The highest cell division was achieved after treatment of 22Rv1 and PC-3 cells with sarcosine (time required for division decreased by 65% or 45%, when compared to untreated cells). qRT-PCR revealed also significant effects on expression of GNMT. Finally, amino acid profiling shown specific amino acid patterns for each cell line. In both, treated and untreated PC-3 cells significantly higher levels of serine, glutamic acid, and aspartate, linked with prostate cancer progression were found. CONCLUSIONS Sarcosine-related amino acids can exceptionally affect the behavior of benign and malignant prostate cells.
ER -
HEGER, Zbynek, Jaromír GUMULEC, Natalia CERNEI, Hana POLANSKÁ, Martina RAUDENSKÁ, Michal MASAŘÍK, Tomas ECKSCHLAGER, Marie STIBOROVA, Vojtech ADAM and Rene KIZEK. Relation of exposure to amino acids involved in sarcosine metabolic pathway on behavior of non-tumor and malignant prostatic cell lines. \textit{Prostate}. Hoboken: Wiley-Blackwell, 2016, vol.~76, No~7, p.~679-690. ISSN~0270-4137. Available from: https://dx.doi.org/10.1002/pros.23159.
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