Classification of Aeromonas spp. isolated from water and
clinical sources and distribution of virulence genes

J 2016

Classification of Aeromonas spp. isolated from water and clinical sources and distribution of virulence genes

KRÁLOVÁ, Stanislava, Eva STAŇKOVÁ and Ivo SEDLÁČEK

Basic information

Original name

Classification of Aeromonas spp. isolated from water and clinical sources and distribution of virulence genes

Authors

KRÁLOVÁ, Stanislava (703 Slovakia, guarantor, belonging to the institution), Eva STAŇKOVÁ (203 Czech Republic, belonging to the institution) and Ivo SEDLÁČEK (203 Czech Republic, belonging to the institution)

Edition

Folia Microbiologica, 2016, 0015-5632

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.521

RIV identification code

RIV/00216224:14310/16:00091233

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1007/s12223-016-0464-9

UT WoS

000386351700009

Keywords in English

Aeromonas; virulence genes; virulence factors; identification; multiplex PCR;

Abstract

V originále

In this work, 84 isolates of aeromonads were isolated from water and clinical samples, identified, and characterized. Identification was based on routine phenotyping combined with multiplex PCR. In this study, multiplex PCR was retested and reevaluated and its identification key was enhanced by 17 newly described species and five subspecies. Identification score increased from 36 % (only phenotyping) to 90% when supported with multiplex PCR. Further description of isolates included detection of eight virulence genes. These genes were overall present in 46 % (act), 2.4 % (ast), 80%(alt), 40%(ahh1), 20%(asa1), 69%(pla/lip/lipH3/alp- 1), 69 % (ser), and 81 % (fla), and no significant differences between water and clinical isolates were found. Results of this work show that the proper combination of different approaches is necessary for final identification of Aeromonas spp. at the species level. Multiplex PCR was shown to have limits in final identification, specifically inability to distinguish four species pairs and one triplet as their gene profiles are identical. However, it seems to be rapid and easy to do method able to support routine biochemical identification in laboratories. Moreover, our results supported previous proposal of reclassification of BAeromonas hydrophila subsp. dhakensis^ and BAeromonas aquariorum^ as identical species.

Links

EE2.3.20.0183, research and development project

Name: Centrum experimentální biomedicíny

MUNI/A/0884/2013, interní kód MU

Name: Podpora výzkumných aktivit studentů mikrobiologie (Acronym: MIKROB)

Investor: Masaryk University, Category A

Citovat

KRÁLOVÁ, Stanislava, Eva STAŇKOVÁ and Ivo SEDLÁČEK. Classification of Aeromonas spp. isolated from water and clinical sources and distribution of virulence genes. Folia Microbiologica. 2016, vol. 61, No 6, p. 513-521. ISSN 0015-5632. Available from: https://dx.doi.org/10.1007/s12223-016-0464-9.

@article{1356887,
   author = {Králová, Stanislava and Staňková, Eva and Sedláček, Ivo},
   article_number = {6},
   doi = {http://dx.doi.org/10.1007/s12223-016-0464-9},
   keywords = {Aeromonas; virulence genes; virulence factors; identification; multiplex PCR;},
   language = {eng},
   issn = {0015-5632},
   journal = {Folia Microbiologica},
   title = {Classification of Aeromonas spp. isolated from water and clinical sources and distribution of virulence genes},
   volume = {61},
   year = {2016}
}

TY  - JOUR
ID  - 1356887
AU  - Králová, Stanislava - Staňková, Eva - Sedláček, Ivo
PY  - 2016
TI  - Classification of Aeromonas spp. isolated from water and clinical sources and distribution of virulence genes
JF  - Folia Microbiologica
VL  - 61
IS  - 6
SP  - 513-521
EP  - 513-521
SN  - 00155632
KW  - Aeromonas
KW  - virulence genes
KW  - virulence factors
KW  - identification
KW  - multiplex PCR;
N2  - In this work, 84 isolates of aeromonads were isolated from water and clinical samples, identified, and characterized. Identification was based on routine phenotyping combined with multiplex PCR. In this study, multiplex PCR was retested and reevaluated and its identification key was enhanced by 17 newly described species and five subspecies. Identification score increased from 36 % (only phenotyping) to 90% when supported with multiplex PCR. Further description of isolates included detection of eight virulence genes. These genes were overall present in 46 % (act), 2.4 % (ast), 80%(alt), 40%(ahh1), 20%(asa1), 69%(pla/lip/lipH3/alp- 1), 69 % (ser), and 81 % (fla), and no significant differences between water and clinical isolates were found. Results of this work show that the proper combination of different approaches is necessary for final identification of Aeromonas spp. at the species level. Multiplex PCR was shown to have limits in final identification, specifically inability to distinguish four species pairs and one triplet as their gene profiles are identical. However, it seems to be rapid and easy to do method able to support routine biochemical identification in laboratories. Moreover, our results supported previous proposal of reclassification of BAeromonas hydrophila subsp. dhakensis^ and BAeromonas aquariorum^ as identical species.
ER  -

KRÁLOVÁ, Stanislava, Eva STAŇKOVÁ and Ivo SEDLÁČEK. Classification of Aeromonas spp. isolated from water and clinical sources and distribution of virulence genes. \textit{Folia Microbiologica}. 2016, vol.~61, No~6, p.~513-521. ISSN~0015-5632. Available from: https://dx.doi.org/10.1007/s12223-016-0464-9.

Detailed Information on Publication Record