a 2016

Structural and functional determination of predicted core fucose specific mutants of Ralstonia solanacearum lectin

MELICHER, Filip, Deepti MISHRA, Jan KOMÁREK a Michaela WIMMEROVÁ

Základní údaje

Originální název

Structural and functional determination of predicted core fucose specific mutants of Ralstonia solanacearum lectin

Autoři

MELICHER, Filip (703 Slovensko, domácí), Deepti MISHRA (356 Indie, domácí), Jan KOMÁREK (203 Česká republika, domácí) a Michaela WIMMEROVÁ (203 Česká republika, garant)

Vydání

XXV. Biochemický szjezd, 2016

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/16:00088252

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-270-0331-0

Klíčová slova anglicky

Ralstinia solanacearum; lectin; mutagenesis; structural; functional; core fucose;
Změněno: 19. 10. 2016 14:29, Mgr. Filip Melicher

Anotace

V originále

Lectins (from Latin, legere, to select or choose) are multivalent proteins with the ability to recognize and reversibly bind mono- and oligosacharides. The sugar binding sites of lectins, called carbohydrate recognition domain (CRD), promote specific recognition in accordance with the key-lock model. Mutagenesis in CRD may lead to the improvement of binding specificity of lectins, making them markers for carbohydrate structural motifs in nature. Ralstonia solanacearum lectin (RSL) isolated from Ralstonia solanacearum, a phytopathogen causing lethal wilting of agricultural crops, is a trimeric L-fucose specific lectin with the six bladed b-propeller fold. Each monomer presents two fucose specific binding sites, resulting in six symmetrically arranged CRDs. Core fucosylation is the most important core modification in vertebrate N-glycans. It is the addition of fucose via a1-6 linkage to the N-acetylglucosamine adjecent to asparagine in the core. Core fucosylation is involved in many physiological events such as growth and cell differentiation. Recent studies revealed that increasing level of core fucosylation is associated with some diseases (for instance hepatocellular carcinoma). Our work is focused on structural and functional characterization of RSL mutants that might show (based on the results of in silico mutagenesis and docking) higher specificity towards core fucose. Mutagenesis was performed by PCR and the affinity towards different fucose derivatives was measured by isothermal titration calorimetry (ITC) and microscale thermoforesis (MST). Structural changes in CRD after mutagenesis, were determined by protein crystallography.

Návaznosti

GA13-25401S, projekt VaV
Název: Studium proteinů z patogenů zapojených do rozpoznávání hostitelského organismu
Investor: Grantová agentura ČR, Studium proteinu z patogenu zapojených do rozpoznávání hostitelského organismu
Zobrazeno: 7. 11. 2024 09:06