Structural and functional determination of predicted core
fucose specific mutants of Ralstonia solanacearum lectin

a 2016

Structural and functional determination of predicted core fucose specific mutants of Ralstonia solanacearum lectin

MELICHER, Filip, Deepti MISHRA, Jan KOMÁREK and Michaela WIMMEROVÁ

Basic information

Original name

Structural and functional determination of predicted core fucose specific mutants of Ralstonia solanacearum lectin

Authors

MELICHER, Filip (703 Slovakia, belonging to the institution), Deepti MISHRA (356 India, belonging to the institution), Jan KOMÁREK (203 Czech Republic, belonging to the institution) and Michaela WIMMEROVÁ (203 Czech Republic, guarantor)

Edition

XXV. Biochemický szjezd, 2016

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/16:00088252

Organization unit

Faculty of Science

ISBN

978-80-270-0331-0

Keywords in English

Ralstinia solanacearum; lectin; mutagenesis; structural; functional; core fucose;

Změněno: 19/10/2016 14:29, Mgr. Filip Melicher

Abstract

V originále

Lectins (from Latin, legere, to select or choose) are multivalent proteins with the ability to recognize and reversibly bind mono- and oligosacharides. The sugar binding sites of lectins, called carbohydrate recognition domain (CRD), promote specific recognition in accordance with the key-lock model. Mutagenesis in CRD may lead to the improvement of binding specificity of lectins, making them markers for carbohydrate structural motifs in nature. Ralstonia solanacearum lectin (RSL) isolated from Ralstonia solanacearum, a phytopathogen causing lethal wilting of agricultural crops, is a trimeric L-fucose specific lectin with the six bladed b-propeller fold. Each monomer presents two fucose specific binding sites, resulting in six symmetrically arranged CRDs. Core fucosylation is the most important core modification in vertebrate N-glycans. It is the addition of fucose via a1-6 linkage to the N-acetylglucosamine adjecent to asparagine in the core. Core fucosylation is involved in many physiological events such as growth and cell differentiation. Recent studies revealed that increasing level of core fucosylation is associated with some diseases (for instance hepatocellular carcinoma). Our work is focused on structural and functional characterization of RSL mutants that might show (based on the results of in silico mutagenesis and docking) higher specificity towards core fucose. Mutagenesis was performed by PCR and the affinity towards different fucose derivatives was measured by isothermal titration calorimetry (ITC) and microscale thermoforesis (MST). Structural changes in CRD after mutagenesis, were determined by protein crystallography.

Links

GA13-25401S, research and development project

Name: Studium proteinů z patogenů zapojených do rozpoznávání hostitelského organismu

Investor: Czech Science Foundation

Citovat

MELICHER, Filip, Deepti MISHRA, Jan KOMÁREK and Michaela WIMMEROVÁ. Structural and functional determination of predicted core fucose specific mutants of Ralstonia solanacearum lectin. In XXV. Biochemický szjezd. 2016. ISBN 978-80-270-0331-0.

@proceedings{1357332,
   author = {Melicher, Filip and Mishra, Deepti and Komárek, Jan and Wimmerová, Michaela},
   booktitle = {XXV. Biochemický szjezd},
   keywords = {Ralstinia solanacearum; lectin; mutagenesis; structural; functional; core fucose;},
   language = {eng},
   isbn = {978-80-270-0331-0},
   title = {Structural and functional determination of predicted core fucose specific mutants of Ralstonia solanacearum lectin},
   year = {2016}
}

TY  - CONF
ID  - 1357332
AU  - Melicher, Filip - Mishra, Deepti - Komárek, Jan - Wimmerová, Michaela
PY  - 2016
TI  - Structural and functional determination of predicted core fucose specific mutants of Ralstonia solanacearum lectin
SN  - 9788027003310
KW  - Ralstinia solanacearum
KW  - lectin
KW  - mutagenesis
KW  - structural
KW  - functional
KW  - core fucose;
N2  - Lectins (from Latin, legere, to select or choose) are multivalent proteins with the ability to recognize and reversibly bind mono- and oligosacharides. The sugar binding sites of lectins, called carbohydrate recognition domain (CRD), promote specific recognition in accordance with the key-lock model. Mutagenesis in CRD may lead to the improvement of binding specificity of lectins, making them markers for carbohydrate structural motifs in nature. Ralstonia solanacearum lectin (RSL) isolated from Ralstonia solanacearum, a phytopathogen causing lethal wilting of agricultural crops, is a trimeric L-fucose specific lectin with the six bladed b-propeller fold. Each monomer presents two fucose specific binding sites, resulting in six symmetrically arranged CRDs. Core fucosylation is the most important core modification in vertebrate N-glycans. It is the addition of fucose via a1-6 linkage to the N-acetylglucosamine adjecent to asparagine in the core. Core fucosylation is involved in many physiological events such as growth and cell differentiation. Recent studies revealed that increasing level of core fucosylation is associated with some diseases (for instance hepatocellular carcinoma). Our work is focused on structural and functional characterization of RSL mutants that might show (based on the results of in silico mutagenesis and docking) higher specificity towards core fucose. Mutagenesis was performed by PCR and the affinity towards different fucose derivatives was measured by isothermal titration calorimetry (ITC) and microscale thermoforesis (MST). Structural changes in CRD after mutagenesis, were determined by protein crystallography.
ER  -

MELICHER, Filip, Deepti MISHRA, Jan KOMÁREK and Michaela WIMMEROVÁ. Structural and functional determination of predicted core fucose specific mutants of Ralstonia solanacearum lectin. In \textit{XXV. Biochemický szjezd}. 2016. ISBN~978-80-270-0331-0.

Detailed Information on Publication Record