Microcin determinants are associated with B2 phylogroup of
human fecal Escherichia coli isolates

J 2016

Microcin determinants are associated with B2 phylogroup of human fecal Escherichia coli isolates

MICENKOVÁ, Lenka, Juraj BOSÁK, Barbora ŠTAUDOVÁ, D. KOHOUTOVA, D. CEJKOVA et. al.

Basic information

Original name

Microcin determinants are associated with B2 phylogroup of human fecal Escherichia coli isolates

Authors

MICENKOVÁ, Lenka (703 Slovakia, belonging to the institution), Juraj BOSÁK (703 Slovakia, belonging to the institution), Barbora ŠTAUDOVÁ (203 Czech Republic, belonging to the institution), D. KOHOUTOVA (203 Czech Republic), D. CEJKOVA (203 Czech Republic), Vladana WOZNICOVÁ (203 Czech Republic, belonging to the institution), M. VRBA (203 Czech Republic), Alena ŠEVČÍKOVÁ (203 Czech Republic, belonging to the institution), J. BURES (203 Czech Republic) and David ŠMAJS (203 Czech Republic, guarantor, belonging to the institution)

Edition

MicrobiologyOpen, Hoboken, Wiley-Blackwell, 2016, 2045-8827

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.747

RIV identification code

RIV/00216224:14110/16:00100694

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1002/mbo3.345

UT WoS

000378920700012

Keywords in English

Bacteriocin; colicin; E; coli; microcin; phylogroup

Abstract

V originále

Escherichia coli strains are classified into four main phylogenetic groups (A, B1, B2, and D) and strains of these phylogroups differ in a number of characteristics. This study tested whether human fecal E. coli isolates belonging to different phylogroups differ in prevalence of bacteriocinogenic isolates and prevalence of individual bacteriocinogenic determinants. A set of 1283 fecal E. coli isolates from patients with different diseases was tested for the presence of DNA regions allowing classification into E. coli phylogroups and for the ability to produce bacteriocins (23 colicins and 7 microcins). Of the isolates tested, the most common was phylogroup B2 (38.3%) followed by phylogroups A (28.3%), D (26.3%) and B1 (7.2%). Altogether, 695 bacteriocin producers were identified representing 54.2% of all tested isolates. The highest prevalence of bacteriocin producers was found in group B2 (60.3%) and the lowest in group B1 (44.6%). Determinants encoding colicins E1, Ia, and microcin mV were most common in phylogroup A, determinants encoding microcins mM and mH47 were most common in phylogroup B2, and determinant encoding mB17 was most common in phylogroup D. The highest prevalence of bacteriocinogeny was found in phylogroup B2, suggesting that bacteriocinogeny and especially the synthesis of microcins was associated with virulent and resident E. coli strains.

Links

GA16-21649S, research and development project

Name: Molekulární charakterizace nových bakteriocinů identifikovaných v rodech Escherichia a Shigella

Investor: Czech Science Foundation

NT13413, research and development project

Name: Stanovení apoptózy v biopticky odebraných vzorcích z tlustého střeva

Investor: Ministry of Health of the CR

Citovat

MICENKOVÁ, Lenka, Juraj BOSÁK, Barbora ŠTAUDOVÁ, D. KOHOUTOVA, D. CEJKOVA, Vladana WOZNICOVÁ, M. VRBA, Alena ŠEVČÍKOVÁ, J. BURES and David ŠMAJS. Microcin determinants are associated with B2 phylogroup of human fecal Escherichia coli isolates. MicrobiologyOpen. Hoboken: Wiley-Blackwell, 2016, vol. 5, No 3, p. 490-498. ISSN 2045-8827. Available from: https://dx.doi.org/10.1002/mbo3.345.

@article{1357485,
   author = {Micenková, Lenka and Bosák, Juraj and Štaudová, Barbora and Kohoutova, D. and Cejkova, D. and Woznicová, Vladana and Vrba, M. and Ševčíková, Alena and Bures, J. and Šmajs, David},
   article_location = {Hoboken},
   article_number = {3},
   doi = {http://dx.doi.org/10.1002/mbo3.345},
   keywords = {Bacteriocin; colicin; E; coli; microcin; phylogroup},
   language = {eng},
   issn = {2045-8827},
   journal = {MicrobiologyOpen},
   title = {Microcin determinants are associated with B2 phylogroup of human fecal Escherichia coli isolates},
   volume = {5},
   year = {2016}
}

TY  - JOUR
ID  - 1357485
AU  - Micenková, Lenka - Bosák, Juraj - Štaudová, Barbora - Kohoutova, D. - Cejkova, D. - Woznicová, Vladana - Vrba, M. - Ševčíková, Alena - Bures, J. - Šmajs, David
PY  - 2016
TI  - Microcin determinants are associated with B2 phylogroup of human fecal Escherichia coli isolates
JF  - MicrobiologyOpen
VL  - 5
IS  - 3
SP  - 490-498
EP  - 490-498
PB  - Wiley-Blackwell
SN  - 20458827
KW  - Bacteriocin
KW  - colicin
KW  - E
KW  - coli
KW  - microcin
KW  - phylogroup
N2  - Escherichia coli strains are classified into four main phylogenetic groups (A, B1, B2, and D) and strains of these phylogroups differ in a number of characteristics. This study tested whether human fecal E. coli isolates belonging to different phylogroups differ in prevalence of bacteriocinogenic isolates and prevalence of individual bacteriocinogenic determinants. A set of 1283 fecal E. coli isolates from patients with different diseases was tested for the presence of DNA regions allowing classification into E. coli phylogroups and for the ability to produce bacteriocins (23 colicins and 7 microcins). Of the isolates tested, the most common was phylogroup B2 (38.3%) followed by phylogroups A (28.3%), D (26.3%) and B1 (7.2%). Altogether, 695 bacteriocin producers were identified representing 54.2% of all tested isolates. The highest prevalence of bacteriocin producers was found in group B2 (60.3%) and the lowest in group B1 (44.6%). Determinants encoding colicins E1, Ia, and microcin mV were most common in phylogroup A, determinants encoding microcins mM and mH47 were most common in phylogroup B2, and determinant encoding mB17 was most common in phylogroup D. The highest prevalence of bacteriocinogeny was found in phylogroup B2, suggesting that bacteriocinogeny and especially the synthesis of microcins was associated with virulent and resident E. coli strains.
ER  -

MICENKOVÁ, Lenka, Juraj BOSÁK, Barbora ŠTAUDOVÁ, D. KOHOUTOVA, D. CEJKOVA, Vladana WOZNICOVÁ, M. VRBA, Alena ŠEVČÍKOVÁ, J. BURES and David ŠMAJS. Microcin determinants are associated with B2 phylogroup of human fecal Escherichia coli isolates. \textit{MicrobiologyOpen}. Hoboken: Wiley-Blackwell, 2016, vol.~5, No~3, p.~490-498. ISSN~2045-8827. Available from: https://dx.doi.org/10.1002/mbo3.345.

Detailed Information on Publication Record