Termination of Shoot Gravitropic Responses by Auxin Feedback on
PIN3 Polarity

J 2016

Termination of Shoot Gravitropic Responses by Auxin Feedback on PIN3 Polarity

RAKUSOVÁ, Hana, Mohamad ABBAS, Huibin HAN, Siyuan SONG, Helene ROBERT BOISIVON et. al.

Basic information

Original name

Termination of Shoot Gravitropic Responses by Auxin Feedback on PIN3 Polarity

Authors

RAKUSOVÁ, Hana (203 Czech Republic), Mohamad ABBAS (818 Egypt), Huibin HAN (156 China), Siyuan SONG (156 China), Helene ROBERT BOISIVON (250 France, guarantor, belonging to the institution) and Jiří FRIML (203 Czech Republic)

Edition

Current Biology, Cambridge, Cell Press, 2016, 0960-9822

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 8.851

RIV identification code

RIV/00216224:14740/16:00088262

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.cub.2016.08.067

UT WoS

000388545900020

Keywords in English

gravitropism; Arabidopsis; hypocotyl; auxin; auxin transport; auxin feedback; PIN polarity

Abstract

V originále

Plants adjust their growth according to gravity. Gravitropism involves gravity perception, signal transduction, and asymmetric growth response, with organ bending as a consequence [1]. Asymmetric growth results from the asymmetric distribution of the plant-specific signaling molecule auxin [2] that is generated by lateral transport, mediated in the hypocotyl predominantly by the auxin transporter PIN-FORMED3 (PIN3) [3, 4, 5]. Gravity stimulation polarizes PIN3 to the bottom sides of endodermal cells, correlating with increased auxin accumulation in adjacent tissues at the lower side of the stimulated organ, where auxin induces cell elongation and, hence, organ bending. A curvature response allows the hypocotyl to resume straight growth at a defined angle [6], implying that at some point auxin symmetry is restored to prevent overbending. Here, we present initial insights into cellular and molecular mechanisms that lead to the termination of the tropic response. We identified an auxin feedback on PIN3 polarization as underlying mechanism that restores symmetry of the PIN3-dependent auxin flow. Thus, two mechanistically distinct PIN3 polarization events redirect auxin fluxes at different time points of the gravity response: first, gravity-mediated redirection of PIN3-mediated auxin flow toward the lower hypocotyl side, where auxin gradually accumulates and promotes growth, and later PIN3 polarization to the opposite cell side, depleting this auxin maximum to end the bending. Accordingly, genetic or pharmacological interference with the late PIN3 polarization prevents termination of the response and leads to hypocotyl overbending. This observation reveals a role of auxin feedback on PIN polarity in the termination of the tropic response.

Links

GA13-40637S, research and development project

Name: Genetické studie k identifikaci molekulárních mechanizmů buněčné polarity a auxinového transportu v rostlinách

Investor: Czech Science Foundation

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

RAKUSOVÁ, Hana, Mohamad ABBAS, Huibin HAN, Siyuan SONG, Helene ROBERT BOISIVON and Jiří FRIML. Termination of Shoot Gravitropic Responses by Auxin Feedback on PIN3 Polarity. Current Biology. Cambridge: Cell Press, 2016, vol. 26, No 22, p. 3026-3032. ISSN 0960-9822. Available from: https://dx.doi.org/10.1016/j.cub.2016.08.067.

@article{1357600,
   author = {Rakusová, Hana and Abbas, Mohamad and Han, Huibin and Song, Siyuan and Robert Boisivon, Helene and Friml, Jiří},
   article_location = {Cambridge},
   article_number = {22},
   doi = {http://dx.doi.org/10.1016/j.cub.2016.08.067},
   keywords = {gravitropism; Arabidopsis; hypocotyl; auxin; auxin transport; auxin feedback; PIN polarity},
   language = {eng},
   issn = {0960-9822},
   journal = {Current Biology},
   title = {Termination of Shoot Gravitropic Responses by Auxin Feedback on PIN3 Polarity},
   url = {http://www.cell.com/action/showImagesData?pii=S0960-9822%2816%2931012-0},
   volume = {26},
   year = {2016}
}

TY  - JOUR
ID  - 1357600
AU  - Rakusová, Hana - Abbas, Mohamad - Han, Huibin - Song, Siyuan - Robert Boisivon, Helene - Friml, Jiří
PY  - 2016
TI  - Termination of Shoot Gravitropic Responses by Auxin Feedback on PIN3 Polarity
JF  - Current Biology
VL  - 26
IS  - 22
SP  - 3026-3032
EP  - 3026-3032
PB  - Cell Press
SN  - 09609822
KW  - gravitropism
KW  - Arabidopsis
KW  - hypocotyl
KW  - auxin
KW  - auxin transport
KW  - auxin feedback
KW  - PIN polarity
UR  - http://www.cell.com/action/showImagesData?pii=S0960-9822%2816%2931012-0
L2  - http://www.cell.com/action/showImagesData?pii=S0960-9822%2816%2931012-0
N2  - Plants adjust their growth according to gravity. Gravitropism involves gravity perception, signal transduction, and asymmetric growth response, with organ bending as a consequence [1]. Asymmetric growth results from the asymmetric distribution of the plant-specific signaling molecule auxin [2] that is generated by lateral transport, mediated in the hypocotyl predominantly by the auxin transporter PIN-FORMED3 (PIN3) [3, 4, 5]. Gravity stimulation polarizes PIN3 to the bottom sides of endodermal cells, correlating with increased auxin accumulation in adjacent tissues at the lower side of the stimulated organ, where auxin induces cell elongation and, hence, organ bending. A curvature response allows the hypocotyl to resume straight growth at a defined angle [6], implying that at some point auxin symmetry is restored to prevent overbending. Here, we present initial insights into cellular and molecular mechanisms that lead to the termination of the tropic response. We identified an auxin feedback on PIN3 polarization as underlying mechanism that restores symmetry of the PIN3-dependent auxin flow. Thus, two mechanistically distinct PIN3 polarization events redirect auxin fluxes at different time points of the gravity response: first, gravity-mediated redirection of PIN3-mediated auxin flow toward the lower hypocotyl side, where auxin gradually accumulates and promotes growth, and later PIN3 polarization to the opposite cell side, depleting this auxin maximum to end the bending. Accordingly, genetic or pharmacological interference with the late PIN3 polarization prevents termination of the response and leads to hypocotyl overbending. This observation reveals a role of auxin feedback on PIN polarity in the termination of the tropic response.
ER  -

RAKUSOVÁ, Hana, Mohamad ABBAS, Huibin HAN, Siyuan SONG, Helene ROBERT BOISIVON and Jiří FRIML. Termination of Shoot Gravitropic Responses by Auxin Feedback on PIN3 Polarity. \textit{Current Biology}. Cambridge: Cell Press, 2016, vol.~26, No~22, p.~3026-3032. ISSN~0960-9822. Available from: https://dx.doi.org/10.1016/j.cub.2016.08.067.

Detailed Information on Publication Record