Human extraintestinal pathogenic Escherichia coli strains
differ in prevalence of virulence factors, phylogroups, and
bacteriocin determinants

J 2016

Human extraintestinal pathogenic Escherichia coli strains differ in prevalence of virulence factors, phylogroups, and bacteriocin determinants

MICENKOVÁ, Lenka, Juraj BOSÁK, Martin VRBA, Alena ŠEVČÍKOVÁ, David ŠMAJS et. al.

Basic information

Original name

Human extraintestinal pathogenic Escherichia coli strains differ in prevalence of virulence factors, phylogroups, and bacteriocin determinants

Authors

MICENKOVÁ, Lenka (703 Slovakia, belonging to the institution), Juraj BOSÁK (703 Slovakia, belonging to the institution), Martin VRBA (203 Czech Republic), Alena ŠEVČÍKOVÁ (203 Czech Republic) and David ŠMAJS (203 Czech Republic, belonging to the institution)

Edition

BMC Microbiology, London, BioMed Central, 2016, 1471-2180

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.644

RIV identification code

RIV/00216224:14110/16:00094564

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1186/s12866-016-0835-z

UT WoS

000383425800002

Keywords in English

Escherichia coli; ExPEC; Colicin; Microcin; Virulence factor; Bacteriocinogeny

Abstract

V originále

Background: The study used a set of 407 human extraintestinal pathogenic E. coli strains (ExPEC) isolated from (1) skin and soft tissue infections, (2) respiratory infections, (3) intra-abdominal infections, and (4) genital smears. The set was tested for bacteriocin production, for prevalence of bacteriocin and virulence determinants, and for phylogenetic typing. Results obtained from the group of ExPEC strains were compared to data from our previously published analyses of 1283 fecal commensal E. coli strains. Results: The frequency of bacteriocinogeny was significantly higher in the set of ExPEC strains (63.1 %), compared to fecal E. coli (54.2 %; p < 0.01). Microcin producers and microcin determinants dominated in ExPEC strains, while colicin producers and colicin determinants were more frequent in fecal E. coli (p < 0.01). Higher production of microcin M and lower production of microcin B17, colicin Ib, and Js was detected in the set of ExPEC strains. ExPEC strains had a significantly higher prevalence of phylogenetic group B2 (52.6 %) compared to fecal E. coli strains (38.3 %; p < 0.01). Conclusions: Human ExPEC strains were shown to differ from human fecal strains in a number of parameters including bacteriocin production, prevalence of several bacteriocin and virulence determinants, and prevalence of phylogenetic groups. Differences in these parameters were also identified within subgroups of ExPEC strains of diverse origin. While some microcin determinants (mM, mH47) were associated with virulent strains, other bacteriocin types (mB17, Ib, and Js) were associated with fecal flora.

Links

GA16-21649S, research and development project

Name: Molekulární charakterizace nových bakteriocinů identifikovaných v rodech Escherichia a Shigella

Investor: Czech Science Foundation

NT13413, research and development project

Name: Stanovení apoptózy v biopticky odebraných vzorcích z tlustého střeva

Investor: Ministry of Health of the CR

Citovat

MICENKOVÁ, Lenka, Juraj BOSÁK, Martin VRBA, Alena ŠEVČÍKOVÁ and David ŠMAJS. Human extraintestinal pathogenic Escherichia coli strains differ in prevalence of virulence factors, phylogroups, and bacteriocin determinants. BMC Microbiology. London: BioMed Central, 2016, vol. 16, No 218, p. 1-8. ISSN 1471-2180. Available from: https://dx.doi.org/10.1186/s12866-016-0835-z.

@article{1358321,
   author = {Micenková, Lenka and Bosák, Juraj and Vrba, Martin and Ševčíková, Alena and Šmajs, David},
   article_location = {London},
   article_number = {218},
   doi = {http://dx.doi.org/10.1186/s12866-016-0835-z},
   keywords = {Escherichia coli; ExPEC; Colicin; Microcin; Virulence factor; Bacteriocinogeny},
   language = {eng},
   issn = {1471-2180},
   journal = {BMC Microbiology},
   title = {Human extraintestinal pathogenic Escherichia coli strains differ in prevalence of virulence factors, phylogroups, and bacteriocin determinants},
   volume = {16},
   year = {2016}
}

TY  - JOUR
ID  - 1358321
AU  - Micenková, Lenka - Bosák, Juraj - Vrba, Martin - Ševčíková, Alena - Šmajs, David
PY  - 2016
TI  - Human extraintestinal pathogenic Escherichia coli strains differ in prevalence of virulence factors, phylogroups, and bacteriocin determinants
JF  - BMC Microbiology
VL  - 16
IS  - 218
SP  - 1-8
EP  - 1-8
PB  - BioMed Central
SN  - 14712180
KW  - Escherichia coli
KW  - ExPEC
KW  - Colicin
KW  - Microcin
KW  - Virulence factor
KW  - Bacteriocinogeny
N2  - Background: The study used a set of 407 human extraintestinal pathogenic E. coli strains (ExPEC) isolated from (1) skin and soft tissue infections, (2) respiratory infections, (3) intra-abdominal infections, and (4) genital smears. The set was tested for bacteriocin production, for prevalence of bacteriocin and virulence determinants, and for phylogenetic typing. Results obtained from the group of ExPEC strains were compared to data from our previously published analyses of 1283 fecal commensal E. coli strains. Results: The frequency of bacteriocinogeny was significantly higher in the set of ExPEC strains (63.1 %), compared to fecal E. coli (54.2 %; p < 0.01). Microcin producers and microcin determinants dominated in ExPEC strains, while colicin producers and colicin determinants were more frequent in fecal E. coli (p < 0.01). Higher production of microcin M and lower production of microcin B17, colicin Ib, and Js was detected in the set of ExPEC strains. ExPEC strains had a significantly higher prevalence of phylogenetic group B2 (52.6 %) compared to fecal E. coli strains (38.3 %; p < 0.01). Conclusions: Human ExPEC strains were shown to differ from human fecal strains in a number of parameters including bacteriocin production, prevalence of several bacteriocin and virulence determinants, and prevalence of phylogenetic groups. Differences in these parameters were also identified within subgroups of ExPEC strains of diverse origin. While some microcin determinants (mM, mH47) were associated with virulent strains, other bacteriocin types (mB17, Ib, and Js) were associated with fecal flora.
ER  -

MICENKOVÁ, Lenka, Juraj BOSÁK, Martin VRBA, Alena ŠEVČÍKOVÁ and David ŠMAJS. Human extraintestinal pathogenic Escherichia coli strains differ in prevalence of virulence factors, phylogroups, and bacteriocin determinants. \textit{BMC Microbiology}. London: BioMed Central, 2016, vol.~16, No~218, p.~1-8. ISSN~1471-2180. Available from: https://dx.doi.org/10.1186/s12866-016-0835-z.

Detailed Information on Publication Record