Sensitivity of photosynthetic processes to freezing temperature
in extremophilic lichens evaluated by linear cooling and
chlorophyll fluorescence

J 2016

Sensitivity of photosynthetic processes to freezing temperature in extremophilic lichens evaluated by linear cooling and chlorophyll fluorescence

HÁJEK, Josef, Miloš BARTÁK, Jana HAZDROVÁ and Marie FORBELSKÁ

Basic information

Original name

Sensitivity of photosynthetic processes to freezing temperature in extremophilic lichens evaluated by linear cooling and chlorophyll fluorescence

Authors

HÁJEK, Josef (203 Czech Republic, guarantor, belonging to the institution), Miloš BARTÁK (203 Czech Republic, belonging to the institution), Jana HAZDROVÁ (203 Czech Republic, belonging to the institution) and Marie FORBELSKÁ (203 Czech Republic, belonging to the institution)

Edition

Cryobiology, Academic Press, 2016, 0011-2240

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.996

RIV identification code

RIV/00216224:14310/16:00091424

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.cryobiol.2016.10.002

UT WoS

000389010600005

Keywords in English

Usnea antarctica; Usnea aurantiaco-atra; Umbilicaria cylindrica; cryoresistance; Antarctica; James Ross Island; temperature stress

Abstract

V originále

Extremophilic lichens and their photosynthesizing photobionts from the cold regions of Earth are adapted to perform photosynthesis at subzero temperatures. To evaluate interspecific differences in the critical temperature for primary photochemical processes of photosynthesis, we exposed lichen thalli of Usnea antarctica, Usnea aurantiaco-atra, and Umbilicaria cylindrica to linear cooling from +20 to minus 50 °C at a constant rate of 2 °C/min. Simultaneously, two chlorophyll fluorescence parameters (FV/FM: potential yield of photosynthetic processes in photosystem II, YieldPSII: effective quantum yield of PS II) evaluating a gradual subzero temperature-induced decline in photosynthetic processes were measured by a modulated fluorometer. For the studied species, the response of FV/FM and YieldPSII to declining temperature showed an S-curve shape. The decline in FV/FM and YieldPSII at low temperatures started at minus 5 and +5 °C, respectively in the majority of cases. The decline was, however, species-specific. U. aurantiaco-atra showed a constant-rate decline of YieldPSII from the physiological temperature 20 °C. U. antarctica exhibited the first sign of FV/FM decline at minus 12 °C. The critical temperature related to full inhibition of the photosynthetic processes in PSII (FV/FM), was found at minus 20 °C. However, this occurred at minus 30 °C for U. cylindrica. In an individual sample, the critical temperature for FV/FM was typically lower than for YieldPSII. The method of linear cooling combined with simultaneous measurements of chlorophyll fluorescence parameters proved to be an efficient tool in the estimation of extremophilic species sensitivity/resistance to freezing.

Links

LM2010009, research and development project

Name: Projekt CzechPolar - České polární stanice: Stavba a operační náklady

Investor: Ministry of Education, Youth and Sports of the CR

LM2015078, research and development project

Name: Česká polární výzkumná infrastruktura (Acronym: CzechPolar2)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

HÁJEK, Josef, Miloš BARTÁK, Jana HAZDROVÁ and Marie FORBELSKÁ. Sensitivity of photosynthetic processes to freezing temperature in extremophilic lichens evaluated by linear cooling and chlorophyll fluorescence. Cryobiology. Academic Press, 2016, vol. 73, No 3, p. 329-334. ISSN 0011-2240. Available from: https://dx.doi.org/10.1016/j.cryobiol.2016.10.002.

@article{1358780,
   author = {Hájek, Josef and Barták, Miloš and Hazdrová, Jana and Forbelská, Marie},
   article_number = {3},
   doi = {http://dx.doi.org/10.1016/j.cryobiol.2016.10.002},
   keywords = {Usnea antarctica; Usnea aurantiaco-atra; Umbilicaria cylindrica; cryoresistance; Antarctica; James Ross Island; temperature stress},
   language = {eng},
   issn = {0011-2240},
   journal = {Cryobiology},
   title = {Sensitivity of photosynthetic processes to freezing temperature in extremophilic lichens evaluated by linear cooling and chlorophyll fluorescence},
   volume = {73},
   year = {2016}
}

TY  - JOUR
ID  - 1358780
AU  - Hájek, Josef - Barták, Miloš - Hazdrová, Jana - Forbelská, Marie
PY  - 2016
TI  - Sensitivity of photosynthetic processes to freezing temperature in extremophilic lichens evaluated by linear cooling and chlorophyll fluorescence
JF  - Cryobiology
VL  - 73
IS  - 3
SP  - 329-334
EP  - 329-334
PB  - Academic Press
SN  - 00112240
KW  - Usnea antarctica
KW  - Usnea aurantiaco-atra
KW  - Umbilicaria cylindrica
KW  - cryoresistance
KW  - Antarctica
KW  - James Ross Island
KW  - temperature stress
N2  - Extremophilic lichens and their photosynthesizing photobionts from the cold regions of Earth are adapted to perform photosynthesis at subzero temperatures. To evaluate interspecific differences in the critical temperature for primary photochemical processes of photosynthesis, we exposed lichen thalli of Usnea antarctica, Usnea aurantiaco-atra, and Umbilicaria cylindrica to linear cooling from +20 to minus 50 °C at a constant rate of 2 °C/min. Simultaneously, two chlorophyll fluorescence parameters (FV/FM: potential yield of photosynthetic processes in photosystem II, YieldPSII: effective quantum yield of PS II) evaluating a gradual subzero temperature-induced decline in photosynthetic processes were measured by a modulated fluorometer. For the studied species, the response of FV/FM and YieldPSII to declining temperature showed an S-curve shape. The decline in FV/FM and YieldPSII at low temperatures started at minus 5 and +5 °C, respectively in the majority of cases. The decline was, however, species-specific. U. aurantiaco-atra showed a constant-rate decline of YieldPSII from the physiological temperature 20 °C. U. antarctica exhibited the first sign of FV/FM decline at minus 12 °C. The critical temperature related to full inhibition of the photosynthetic processes in PSII (FV/FM), was found at minus 20 °C. However, this occurred at minus 30 °C for U. cylindrica. In an individual sample, the critical temperature for FV/FM was typically lower than for YieldPSII. The method of linear cooling combined with simultaneous measurements of chlorophyll fluorescence parameters proved to be an efficient tool in the estimation of extremophilic species sensitivity/resistance to freezing.
ER  -

HÁJEK, Josef, Miloš BARTÁK, Jana HAZDROVÁ and Marie FORBELSKÁ. Sensitivity of photosynthetic processes to freezing temperature in extremophilic lichens evaluated by linear cooling and chlorophyll fluorescence. \textit{Cryobiology}. Academic Press, 2016, vol.~73, No~3, p.~329-334. ISSN~0011-2240. Available from: https://dx.doi.org/10.1016/j.cryobiol.2016.10.002.

Detailed Information on Publication Record