BENCÚROVÁ, Petra, Jiří BALOUN, Kateřina MUSILOVÁ, Karol PÁL, Nikola TOM, Jakub HYNŠT, Lenka RADOVÁ, Martin PAIL, Eva BRICHTOVÁ, Markéta HERMANOVÁ, Šárka POSPÍŠILOVÁ, Marek MRÁZ and Milan BRÁZDIL. Identification of miRNA expression profiles in human mesial temporal lobe epilepsy with hippocampal sclerosis. In 12th European Congress on Epileptology. 2016.
Other formats:   BibTeX LaTeX RIS
Basic information
Original name Identification of miRNA expression profiles in human mesial temporal lobe epilepsy with hippocampal sclerosis
Name in Czech Identifikace miRNA profilů v lidské meziotemporální epilepsií s hipokampální sklerózou
Authors BENCÚROVÁ, Petra (703 Slovakia, belonging to the institution), Jiří BALOUN (203 Czech Republic, belonging to the institution), Kateřina MUSILOVÁ (203 Czech Republic, belonging to the institution), Karol PÁL (203 Czech Republic, belonging to the institution), Nikola TOM (203 Czech Republic, belonging to the institution), Jakub HYNŠT (203 Czech Republic, belonging to the institution), Lenka RADOVÁ (203 Czech Republic, belonging to the institution), Martin PAIL (203 Czech Republic, belonging to the institution), Eva BRICHTOVÁ (203 Czech Republic), Markéta HERMANOVÁ (203 Czech Republic, belonging to the institution), Šárka POSPÍŠILOVÁ (203 Czech Republic, belonging to the institution), Marek MRÁZ (203 Czech Republic, belonging to the institution) and Milan BRÁZDIL (203 Czech Republic, guarantor, belonging to the institution).
Edition 12th European Congress on Epileptology, 2016.
Other information
Original language English
Type of outcome Conference abstract
Field of Study 30000 3. Medical and Health Sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
WWW URL
RIV identification code RIV/00216224:14740/16:00088294
Organization unit Central European Institute of Technology
Keywords (in Czech) miRNA; epilepsie; hipokampální skleróza; sekvenování; miQPCR
Keywords in English miRNA; epilepsy; hippocampal sclerosis; sequencing; miQPCR
Tags International impact
Changed by Changed by: Mgr. Petra Bencúrová, Ph.D., učo 356527. Changed: 23/11/2016 15:58.
Abstract
Purpose: The primary aim of this study was to identify specific miRNAs with aberrant expression in hippocampal tissue of mesial temporal lobe epilepsy (mTLE) patients with hippocampal sclerosis (HS). Method: We analyzed miRNA isolated from paraffin embedded tissue slices of hippocampal complex from 22 mTLE +HS patients (14 left-sided, 8 right-sided) and 9 post mortem controls. To characterize the miRNAs highly prone to be involved in the pathological changes in the mTLE+HS brain tissue we performed the expression analysis combining the next generation sequencing (NGS) and novel specific technique for miRNA quantification miQPCR. Results: By both methodical approaches we confirmed significant upregulation of miR-129-1-3p, 129-2-3p, 142-3p, 191-5p, 193b-3p, 195-5p, 374b-5p, 451a, and 490-3p in mTLE+HS patients. The levels of miRNA-184 were significantly higher in control subjects. Differential expression levels in mTLE+ HS patients were identified also in the case of 10 miRNA species never considered to play a role in epilepsy before. Conclusion: Analytical approach combining NGS with miQPCR used for the first time for analysis of human epileptic foci led to reliable identification of miRNA with altered expression in mTLE +HS patients.
Links
GA16-04726S, research and development projectName: miRNA a biochemické dráhy v epilepsii
Investor: Czech Science Foundation
MUNI/A/1044/2015, interní kód MUName: miRNA jako biomarker meziotemporální epilepsie (Acronym: microRNA jako biomarker MTLE)
Investor: Masaryk University, Category A
PrintDisplayed: 18/7/2024 02:45