BENCÚROVÁ, Petra, Jiří BALOUN, Kateřina MUSILOVÁ, Šárka POSPÍŠILOVÁ, Markéta HERMANOVÁ, Lenka RADOVÁ, Martin PAIL, Eva BRICHTOVÁ, Marek MRÁZ and Milan BRÁZDIL. Whole miRNome Sequencing of Human Epileptic Brain Tissue. In 12th Annual microRNA as Biomarkers and Diagnostics. 2016.
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Basic information
Original name Whole miRNome Sequencing of Human Epileptic Brain Tissue
Name in Czech Celo-miRNomové sekvenování lidské epileptické mozkové tkáně
Authors BENCÚROVÁ, Petra (703 Slovakia, belonging to the institution), Jiří BALOUN (203 Czech Republic, belonging to the institution), Kateřina MUSILOVÁ (203 Czech Republic, belonging to the institution), Šárka POSPÍŠILOVÁ (203 Czech Republic, belonging to the institution), Markéta HERMANOVÁ (203 Czech Republic, belonging to the institution), Lenka RADOVÁ (203 Czech Republic, belonging to the institution), Martin PAIL (203 Czech Republic, belonging to the institution), Eva BRICHTOVÁ (203 Czech Republic), Marek MRÁZ (203 Czech Republic, belonging to the institution) and Milan BRÁZDIL (203 Czech Republic, guarantor, belonging to the institution).
Edition 12th Annual microRNA as Biomarkers and Diagnostics, 2016.
Other information
Original language English
Type of outcome Conference abstract
Field of Study 30000 3. Medical and Health Sciences
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW URL
RIV identification code RIV/00216224:14740/16:00088295
Organization unit Central European Institute of Technology
Keywords (in Czech) miRNA; epilepsie; hipokampální skleróza; sekvenování; qRT-PCR
Keywords in English miRNA; epilepsy; hippocampal sclerosis; sequencing; qRT-PCR
Tags International impact
Changed by Changed by: Mgr. Petra Bencúrová, Ph.D., učo 356527. Changed: 8/1/2018 11:18.
Abstract
Mesial temporal lobe epilepsy (mTLE) is a chronic neurological disorder characterized by recurrent seizures. Accumulating evidence of microRNAs´ (miRNAs) potential involvement in pathogenic mechanisms underlying mTLE is emerging. The function of microRNAs in chronic mTLE is still poorly understood partially due to the lack of a clear cut miRNA expression profile of epileptic foci. In the present study, we performed the whole miRNome profiling analysis of the human brain tissue employing novel approach combining next generation sequencing (NGS) with an advanced qPCR technique, miQPCR. miRNA profiles of the hippocampus of autopsy controls and mTLE patients were compared, and analysis revealed differential miRNA expression of 24 miRNAs consistent with published data as well as several miRNA species never considered to play a role in epilepsy before. In conclusion, NGS exposed a group of miRNAs manifesting invariable expression across all samples out of which stable expression of four miRNAs was validated by combined methods for future utilization as negative controls or normalizers.
Links
GA16-04726S, research and development projectName: miRNA a biochemické dráhy v epilepsii
Investor: Czech Science Foundation
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