Detailed Information on Publication Record
2016
Methodical approaches to PCR based quantification of miRNA in epilepsy
BENCÚROVÁ, PetraBasic information
Original name
Methodical approaches to PCR based quantification of miRNA in epilepsy
Name in Czech
Metodické přístupy ke kvantifikaci miRNA v epilepsii na báze PCR
Authors
BENCÚROVÁ, Petra (703 Slovakia, guarantor, belonging to the institution)
Edition
12th European Congress on Epileptology, 2016
Other information
Language
English
Type of outcome
Vyžádané přednášky
Field of Study
30000 3. Medical and Health Sciences
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
RIV identification code
RIV/00216224:14740/16:00088297
Organization unit
Central European Institute of Technology
Keywords (in Czech)
qRT-PCR; sekvenovaní; miRNA; epilepsie; nové metody; NGS knihovny
Keywords in English
qRT-PCR; sequencing; miRNA; epilepsy; new methods; NGS libraries
Tags
International impact
Změněno: 8/1/2018 11:17, Mgr. Petra Bencúrová, Ph.D.
Abstract
V originále
microRNA(miRNA/miR) is a short noncoding RNA regulating gene expression on posttranscriptional level. The proper regulation by miRNA is essential for broad range of processes in the organism. Hence, the altered expression of miRNAs might be involved in various pathologies. Since 2010 is miRNA one of the important topics studied in the field of epilepsy. Altered miRNA profile seems to be contributing to the development of epilepsy and the disease progression. The accurate identification of miRNA profile of epilepsy and its subtypes would considerably contribute to the diagnostics and treatment of the disease. This presentation was focused on the methodical approaches used in the research of miRNA in epilepsy so far and the improvements in contemporary technologies of miRNA profiling. Recent advance in small RNA library preparation for Illumina sequencing reduces the bias in miRNA profile identification. Nevertheless, the validation by an alternative method is necessary for all miRNA profiling platforms in order to yield precise miRNA profile.
Links
| GA16-04726S, research and development project |
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| MUNI/A/1044/2015, interní kód MU |
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