Methodical approaches to PCR based quantification of miRNA in epilepsy

BENCÚROVÁ, Petra. Methodical approaches to PCR based quantification of miRNA in epilepsy. In 12th European Congress on Epileptology. 2016.

Basic information

• Original name: Methodical approaches to PCR based quantification of miRNA in epilepsy
• Name in Czech: Metodické přístupy ke kvantifikaci miRNA v epilepsii na báze PCR
• Authors: BENCÚROVÁ, Petra (703 Slovakia, guarantor, belonging to the institution).
• Edition: 12th European Congress on Epileptology, 2016.

Other information

• Original language: English
• Type of outcome: Requested lectures
• Field of Study: 30000 3. Medical and Health Sciences
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• RIV identification code: RIV/00216224:14740/16:00088297
• Organization unit: Central European Institute of Technology
• Keywords (in Czech): qRT-PCR; sekvenovaní; miRNA; epilepsie; nové metody; NGS knihovny
• Keywords in English: qRT-PCR; sequencing; miRNA; epilepsy; new methods; NGS libraries
• Tags: International impact

Abstract

microRNA(miRNA/miR) is a short noncoding RNA regulating gene expression on posttranscriptional level. The proper regulation by miRNA is essential for broad range of processes in the organism. Hence, the altered expression of miRNAs might be involved in various pathologies. Since 2010 is miRNA one of the important topics studied in the field of epilepsy. Altered miRNA profile seems to be contributing to the development of epilepsy and the disease progression. The accurate identification of miRNA profile of epilepsy and its subtypes would considerably contribute to the diagnostics and treatment of the disease. This presentation was focused on the methodical approaches used in the research of miRNA in epilepsy so far and the improvements in contemporary technologies of miRNA profiling. Recent advance in small RNA library preparation for Illumina sequencing reduces the bias in miRNA profile identification. Nevertheless, the validation by an alternative method is necessary for all miRNA profiling platforms in order to yield precise miRNA profile.

Links

• GA16-04726S, research and development project: Name: miRNA a biochemické dráhy v epilepsii

Investor: Czech Science Foundation

• MUNI/A/1044/2015, interní kód MU: Name: miRNA jako biomarker meziotemporální epilepsie (Acronym: microRNA jako biomarker MTLE)

Investor: Masaryk University, Category A