| ŠÍMA, Michal, Marian NOVOTNÝ, Lukáš PRAVDA, Petra SUMOVÁ, Iva ROHOUŠOVÁ a Petr VOLF. The Diversity of Yellow-Related Proteins in Sand Flies (Diptera: Psychodidae). PLOS ONE. San Francisco: Public Library of Science, 2016, roč. 11, č. 11, s. nestránkováno, 17 s. ISSN 1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0166191. |
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@article{1359593,
author = {Šíma, Michal and Novotný, Marian and Pravda, Lukáš and Sumová, Petra and Rohoušová, Iva and Volf, Petr},
article_location = {San Francisco},
article_number = {11},
doi = {http://dx.doi.org/10.1371/journal.pone.0166191},
keywords = {Sand flies; Glycosylation; Protein tunnels; MOLE; Phlebotomus; Phylogenetic analysis; Yellow-related proteins},
language = {eng},
issn = {1932-6203},
journal = {PLOS ONE},
title = {The Diversity of Yellow-Related Proteins in Sand Flies (Diptera: Psychodidae)},
url = {http://dx.doi.org/10.1371/journal.pone.0166191},
volume = {11},
year = {2016}
}
TY - JOUR
ID - 1359593
AU - Šíma, Michal - Novotný, Marian - Pravda, Lukáš - Sumová, Petra - Rohoušová, Iva - Volf, Petr
PY - 2016
TI - The Diversity of Yellow-Related Proteins in Sand Flies (Diptera: Psychodidae)
JF - PLOS ONE
VL - 11
IS - 11
SP - nestránkováno
EP - nestránkováno
PB - Public Library of Science
SN - 19326203
KW - Sand flies
KW - Glycosylation
KW - Protein tunnels
KW - MOLE
KW - Phlebotomus
KW - Phylogenetic analysis
KW - Yellow-related proteins
UR - http://dx.doi.org/10.1371/journal.pone.0166191
L2 - http://dx.doi.org/10.1371/journal.pone.0166191
N2 - Yellow-related proteins (YRPs) present in sand fly saliva act as affinity binders of bioamines, and help the fly to complete a bloodmeal by scavenging the physiological signals of damaged cells. They are also the main antigens in sand fly saliva and their recombinant form is used as a marker of host exposure to sand flies. Moreover, several salivary proteins and plasmids coding these proteins induce strong immune response in hosts bitten by sand flies and are being used to design protecting vaccines against Leishmania parasites. In this study, thirty two 3D models of different yellow-related proteins from thirteen sand fly species of two genera were constructed based on the known protein structure from Lutzomyia longipalpis. We also studied evolutionary relationships among species based on protein sequences as well as sequence and structural variability of their ligand-binding site. All of these 33 sand fly YRPs shared a similar structure, including a unique tunnel that connects the ligand-binding site with the solvent by two independent paths. However, intraspecific modifications found among these proteins affects the charges of the entrances to the tunnel, the length of the tunnel and its hydrophobicity. We suggest that these structural and sequential differences influence the ligand-binding abilities of these proteins and provide sand flies with a greater number of YRP paralogs with more nuanced answers to bioamines. All these characteristics allow us to better evaluate these proteins with respect to their potential use as part of anti-Leishmania vaccines or as an antigen to measure host exposure to sand flies.
ER -
ŠÍMA, Michal, Marian NOVOTNÝ, Lukáš PRAVDA, Petra SUMOVÁ, Iva ROHOUŠOVÁ a Petr VOLF. The Diversity of Yellow-Related Proteins in Sand Flies (Diptera: Psychodidae). \textit{PLOS ONE}. San Francisco: Public Library of Science, 2016, roč.~11, č.~11, s.~nestránkováno, 17 s. ISSN~1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0166191.
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