ŠÍMA, Michal, Marian NOVOTNÝ, Lukáš PRAVDA, Petra SUMOVÁ, Iva ROHOUŠOVÁ a Petr VOLF. The Diversity of Yellow-Related Proteins in Sand Flies (Diptera: Psychodidae). PLOS ONE, San Francisco: Public Library of Science, 2016, roč. 11, č. 11, s. nestránkováno. ISSN 1932-6203. doi:10.1371/journal.pone.0166191.
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Základní údaje
Originální název The Diversity of Yellow-Related Proteins in Sand Flies (Diptera: Psychodidae)
Autoři ŠÍMA, Michal (203 Česko), Marian NOVOTNÝ (203 Česko), Lukáš PRAVDA (203 Česko, garant, domácí), Petra SUMOVÁ (203 Česko), Iva ROHOUŠOVÁ (203 Česko) a Petr VOLF (203 Česko).
Vydání PLOS ONE, San Francisco, Public Library of Science, 2016, 1932-6203.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor 10600 1.6 Biological sciences
Stát vydavatele Spojené státy
Utajení není předmětem státního či obchodního tajemství
WWW Full Text
Impakt faktor Impact factor: 2.806
Kód RIV RIV/00216224:14740/16:00091548
Organizační jednotka Středoevropský technologický institut
Doi http://dx.doi.org/10.1371/journal.pone.0166191
UT WoS 000386910000106
Klíčová slova anglicky Sand flies; Glycosylation; Protein tunnels; MOLE; Phlebotomus; Phylogenetic analysis; Yellow-related proteins
Štítky rivok
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnila: Mgr. Marie Šípková, DiS., učo 437722. Změněno: 19. 12. 2019 15:30.
Anotace
Yellow-related proteins (YRPs) present in sand fly saliva act as affinity binders of bioamines, and help the fly to complete a bloodmeal by scavenging the physiological signals of damaged cells. They are also the main antigens in sand fly saliva and their recombinant form is used as a marker of host exposure to sand flies. Moreover, several salivary proteins and plasmids coding these proteins induce strong immune response in hosts bitten by sand flies and are being used to design protecting vaccines against Leishmania parasites. In this study, thirty two 3D models of different yellow-related proteins from thirteen sand fly species of two genera were constructed based on the known protein structure from Lutzomyia longipalpis. We also studied evolutionary relationships among species based on protein sequences as well as sequence and structural variability of their ligand-binding site. All of these 33 sand fly YRPs shared a similar structure, including a unique tunnel that connects the ligand-binding site with the solvent by two independent paths. However, intraspecific modifications found among these proteins affects the charges of the entrances to the tunnel, the length of the tunnel and its hydrophobicity. We suggest that these structural and sequential differences influence the ligand-binding abilities of these proteins and provide sand flies with a greater number of YRP paralogs with more nuanced answers to bioamines. All these characteristics allow us to better evaluate these proteins with respect to their potential use as part of anti-Leishmania vaccines or as an antigen to measure host exposure to sand flies.
Návaznosti
LQ1601, projekt VaVNázev: CEITEC 2020 (Akronym: CEITEC2020)
Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní program udržitelnosti II (LQ)
Typ Název Vložil/a Vloženo Práva
journal.pone.0166191.PDF   Verze souboru Pravda, L. 9. 11. 2016

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  • osoba Mgr. Marie Šípková, DiS., učo 437722
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Požádat autora o autorský výtisk Zobrazeno: 14. 7. 2020 19:11