ŠÍMA, Michal, Marian NOVOTNÝ, Lukáš PRAVDA, Petra SUMOVÁ, Iva ROHOUŠOVÁ and Petr VOLF. The Diversity of Yellow-Related Proteins in Sand Flies (Diptera: Psychodidae). PLOS ONE, San Francisco: Public Library of Science, 2016, vol. 11, No 11, p. nestránkováno. ISSN 1932-6203. doi:10.1371/journal.pone.0166191.
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Original name The Diversity of Yellow-Related Proteins in Sand Flies (Diptera: Psychodidae)
Authors ŠÍMA, Michal (203 Czechia), Marian NOVOTNÝ (203 Czechia), Lukáš PRAVDA (203 Czechia, guarantor, belonging to the institution), Petra SUMOVÁ (203 Czechia), Iva ROHOUŠOVÁ (203 Czechia) and Petr VOLF (203 Czechia).
Edition PLOS ONE, San Francisco, Public Library of Science, 2016, 1932-6203.
Other information
Original language English
Type of outcome article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW Full Text
Impact factor Impact factor: 2.806
RIV identification code RIV/00216224:14740/16:00091548
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1371/journal.pone.0166191
UT WoS 000386910000106
Keywords in English Sand flies; Glycosylation; Protein tunnels; MOLE; Phlebotomus; Phylogenetic analysis; Yellow-related proteins
Tags rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Marie Šípková, DiS., učo 437722. Changed: 19. 12. 2019 15:30.
Abstract
Yellow-related proteins (YRPs) present in sand fly saliva act as affinity binders of bioamines, and help the fly to complete a bloodmeal by scavenging the physiological signals of damaged cells. They are also the main antigens in sand fly saliva and their recombinant form is used as a marker of host exposure to sand flies. Moreover, several salivary proteins and plasmids coding these proteins induce strong immune response in hosts bitten by sand flies and are being used to design protecting vaccines against Leishmania parasites. In this study, thirty two 3D models of different yellow-related proteins from thirteen sand fly species of two genera were constructed based on the known protein structure from Lutzomyia longipalpis. We also studied evolutionary relationships among species based on protein sequences as well as sequence and structural variability of their ligand-binding site. All of these 33 sand fly YRPs shared a similar structure, including a unique tunnel that connects the ligand-binding site with the solvent by two independent paths. However, intraspecific modifications found among these proteins affects the charges of the entrances to the tunnel, the length of the tunnel and its hydrophobicity. We suggest that these structural and sequential differences influence the ligand-binding abilities of these proteins and provide sand flies with a greater number of YRP paralogs with more nuanced answers to bioamines. All these characteristics allow us to better evaluate these proteins with respect to their potential use as part of anti-Leishmania vaccines or as an antigen to measure host exposure to sand flies.
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LQ1601, research and development projectName: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR, National Feasibility Programme II
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