J 2016

Selective effect of irreversible electroporation on parenchyma of the pancreas and its vascular structures - an in vivo experiment on a porcine model

SVATOŇ, Roman, Jan HLAVSA, Zdeněk KALA, Vladimír PROCHÁZKA, Katarína GAŠPAROVÁ et. al.

Základní údaje

Originální název

Selective effect of irreversible electroporation on parenchyma of the pancreas and its vascular structures - an in vivo experiment on a porcine model

Autoři

SVATOŇ, Roman (703 Slovensko, garant, domácí), Jan HLAVSA (203 Česká republika, domácí), Zdeněk KALA (203 Česká republika, domácí), Vladimír PROCHÁZKA (203 Česká republika, domácí), Katarína GAŠPAROVÁ (703 Slovensko, domácí), Michal CRHA (203 Česká republika), Alois NEČAS (203 Česká republika), Petr RAUŠER (203 Česká republika), Tomáš ANDRAŠINA (703 Slovensko, domácí), Vlastimil VÁLEK (203 Česká republika, domácí) a Iva SVOBODOVÁ (203 Česká republika, domácí)

Vydání

Acta veterinaria Brno, Brno, Veterinární a farmaceutická univerzita Brno, 2016, 0001-7213

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

40301 Veterinary science

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 0.415

Kód RIV

RIV/00216224:14110/16:00091558

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.2754/avb201685020133

UT WoS

000384441300004

Klíčová slova anglicky

IRE; NanoKnife; ablative methods; vessel; damage

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 11. 11. 2016 15:07, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

Irreversible electroporation is a local, non-thermal ablation method, where short electrical pulses of high voltage lead to changes in cell membrane permeability and cell death. Recent experimental studies have shown that it does not lead to damage of blood vessels, nerves, bile duct or ureters. The aim of our experimental study was to evaluate the negative effect of irreversible electroporation regarding damage to the vascular wall and porcine pancreatic tissue. Irreversible electroporation of the pancreas was performed in 6 pigs after medial laparotomy. Irreversible electroporation was applied to each pig to the splenic lobe of the pancreas in order to assess damage to the pancreatic tissue and to the duodenal lobe of the pancreas to assess damage to the vascular structure of the pancreatic tissue. Higher ablation electric intensity (minimum 500 V/cm - maximum 1,750 V/cm, step 250 V/cm) in 90 mu s pulses was utilized on each pig. After 7 days, macroscopic and microscopic evaluations of en bloc resected specimen (pancreas with duodenum) were performed. During 7 post-ablation days, no deaths or clinical worsening occurred in any of the pigs. Necrotic changes in the pancreatic tissue were recorded at an electric intensity of 750 V/cm. Changes in the outer layers of the wall of the arteries and veins occurred at 1,000 V/cm. Transmural vascular wall damage was not recorded in any case. Irreversible electroporation allows for relatively efficient cell death in the target tissues. Our independent experimental work confirms the safety of this method towards vascular structures located in the ablation zone.
Zobrazeno: 19. 11. 2024 19:33