Selective effect of irreversible electroporation on parenchyma
of the pancreas and its vascular structures - an in vivo
experiment on a porcine model

J 2016

Selective effect of irreversible electroporation on parenchyma of the pancreas and its vascular structures - an in vivo experiment on a porcine model

SVATOŇ, Roman, Jan HLAVSA, Zdeněk KALA, Vladimír PROCHÁZKA, Katarína GAŠPAROVÁ et. al.

Basic information

Original name

Selective effect of irreversible electroporation on parenchyma of the pancreas and its vascular structures - an in vivo experiment on a porcine model

Authors

SVATOŇ, Roman (703 Slovakia, guarantor, belonging to the institution), Jan HLAVSA (203 Czech Republic, belonging to the institution), Zdeněk KALA (203 Czech Republic, belonging to the institution), Vladimír PROCHÁZKA (203 Czech Republic, belonging to the institution), Katarína GAŠPAROVÁ (703 Slovakia, belonging to the institution), Michal CRHA (203 Czech Republic), Alois NEČAS (203 Czech Republic), Petr RAUŠER (203 Czech Republic), Tomáš ANDRAŠINA (703 Slovakia, belonging to the institution), Vlastimil VÁLEK (203 Czech Republic, belonging to the institution) and Iva SVOBODOVÁ (203 Czech Republic, belonging to the institution)

Edition

Acta veterinaria Brno, Brno, Veterinární a farmaceutická univerzita Brno, 2016, 0001-7213

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

40301 Veterinary science

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.415

RIV identification code

RIV/00216224:14110/16:00091558

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.2754/avb201685020133

UT WoS

000384441300004

Keywords in English

IRE; NanoKnife; ablative methods; vessel; damage

Abstract

V originále

Irreversible electroporation is a local, non-thermal ablation method, where short electrical pulses of high voltage lead to changes in cell membrane permeability and cell death. Recent experimental studies have shown that it does not lead to damage of blood vessels, nerves, bile duct or ureters. The aim of our experimental study was to evaluate the negative effect of irreversible electroporation regarding damage to the vascular wall and porcine pancreatic tissue. Irreversible electroporation of the pancreas was performed in 6 pigs after medial laparotomy. Irreversible electroporation was applied to each pig to the splenic lobe of the pancreas in order to assess damage to the pancreatic tissue and to the duodenal lobe of the pancreas to assess damage to the vascular structure of the pancreatic tissue. Higher ablation electric intensity (minimum 500 V/cm - maximum 1,750 V/cm, step 250 V/cm) in 90 mu s pulses was utilized on each pig. After 7 days, macroscopic and microscopic evaluations of en bloc resected specimen (pancreas with duodenum) were performed. During 7 post-ablation days, no deaths or clinical worsening occurred in any of the pigs. Necrotic changes in the pancreatic tissue were recorded at an electric intensity of 750 V/cm. Changes in the outer layers of the wall of the arteries and veins occurred at 1,000 V/cm. Transmural vascular wall damage was not recorded in any case. Irreversible electroporation allows for relatively efficient cell death in the target tissues. Our independent experimental work confirms the safety of this method towards vascular structures located in the ablation zone.

Citovat

SVATOŇ, Roman, Jan HLAVSA, Zdeněk KALA, Vladimír PROCHÁZKA, Katarína GAŠPAROVÁ, Michal CRHA, Alois NEČAS, Petr RAUŠER, Tomáš ANDRAŠINA, Vlastimil VÁLEK and Iva SVOBODOVÁ. Selective effect of irreversible electroporation on parenchyma of the pancreas and its vascular structures - an in vivo experiment on a porcine model. Acta veterinaria Brno. Brno: Veterinární a farmaceutická univerzita Brno, 2016, vol. 85, No 2, p. "133"-"+", 12 pp. ISSN 0001-7213. Available from: https://dx.doi.org/10.2754/avb201685020133.

@article{1359655,
   author = {Svatoň, Roman and Hlavsa, Jan and Kala, Zdeněk and Procházka, Vladimír and Gašparová, Katarína and Crha, Michal and Nečas, Alois and Raušer, Petr and Andrašina, Tomáš and Válek, Vlastimil and Svobodová, Iva},
   article_location = {Brno},
   article_number = {2},
   doi = {http://dx.doi.org/10.2754/avb201685020133},
   keywords = {IRE; NanoKnife; ablative methods; vessel; damage},
   language = {eng},
   issn = {0001-7213},
   journal = {Acta veterinaria Brno},
   title = {Selective effect of irreversible electroporation on parenchyma of the pancreas and its vascular structures - an in vivo experiment on a porcine model},
   volume = {85},
   year = {2016}
}

TY  - JOUR
ID  - 1359655
AU  - Svatoň, Roman - Hlavsa, Jan - Kala, Zdeněk - Procházka, Vladimír - Gašparová, Katarína - Crha, Michal - Nečas, Alois - Raušer, Petr - Andrašina, Tomáš - Válek, Vlastimil - Svobodová, Iva
PY  - 2016
TI  - Selective effect of irreversible electroporation on parenchyma of the pancreas and its vascular structures - an in vivo experiment on a porcine model
JF  - Acta veterinaria Brno
VL  - 85
IS  - 2
SP  - "133"-"+"
EP  - "133"-"+"
PB  - Veterinární a farmaceutická univerzita Brno
SN  - 00017213
KW  - IRE
KW  - NanoKnife
KW  - ablative methods
KW  - vessel
KW  - damage
N2  - Irreversible electroporation is a local, non-thermal ablation method, where short electrical pulses of high voltage lead to changes in cell membrane permeability and cell death. Recent experimental studies have shown that it does not lead to damage of blood vessels, nerves, bile duct or ureters. The aim of our experimental study was to evaluate the negative effect of irreversible electroporation regarding damage to the vascular wall and porcine pancreatic tissue. Irreversible electroporation of the pancreas was performed in 6 pigs after medial laparotomy. Irreversible electroporation was applied to each pig to the splenic lobe of the pancreas in order to assess damage to the pancreatic tissue and to the duodenal lobe of the pancreas to assess damage to the vascular structure of the pancreatic tissue. Higher ablation electric intensity (minimum 500 V/cm - maximum 1,750 V/cm, step 250 V/cm) in 90 mu s pulses was utilized on each pig. After 7 days, macroscopic and microscopic evaluations of en bloc resected specimen (pancreas with duodenum) were performed. During 7 post-ablation days, no deaths or clinical worsening occurred in any of the pigs. Necrotic changes in the pancreatic tissue were recorded at an electric intensity of 750 V/cm. Changes in the outer layers of the wall of the arteries and veins occurred at 1,000 V/cm. Transmural vascular wall damage was not recorded in any case. Irreversible electroporation allows for relatively efficient cell death in the target tissues. Our independent experimental work confirms the safety of this method towards vascular structures located in the ablation zone.
ER  -

SVATOŇ, Roman, Jan HLAVSA, Zdeněk KALA, Vladimír PROCHÁZKA, Katarína GAŠPAROVÁ, Michal CRHA, Alois NEČAS, Petr RAUŠER, Tomáš ANDRAŠINA, Vlastimil VÁLEK and Iva SVOBODOVÁ. Selective effect of irreversible electroporation on parenchyma of the pancreas and its vascular structures - an in vivo experiment on a porcine model. \textit{Acta veterinaria Brno}. Brno: Veterinární a farmaceutická univerzita Brno, 2016, vol.~85, No~2, p.~''133''-''+'', 12 pp. ISSN~0001-7213. Available from: https://dx.doi.org/10.2754/avb201685020133.

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