Other formats:
BibTeX
LaTeX
RIS
@article{1360245, author = {Mlynáriková, Katarína and Šedo, Ondrej and Růžička, Filip and Zdráhal, Zbyněk and Holá, Veronika and Mahelová, Martina}, article_location = {Dordrecht}, article_number = {6}, doi = {http://dx.doi.org/10.1007/s12223-016-0458-7}, keywords = {FLIGHT MASS-SPECTROMETRY; DESORPTION IONIZATION-TIME; STAPHYLOCOCCUS-EPIDERMIDIS; RAMAN-SPECTROSCOPY; SURFACES; BACTERIA; IDENTIFICATION; ALBICANS; EXPRESSION; DATABASE}, language = {eng}, issn = {0015-5632}, journal = {Folia Microbiologica}, title = {Evaluation of capacity to detect ability to form biofilm in Candida parapsilosis sensu stricto strains by MALDI-TOF MS}, volume = {61}, year = {2016} }
TY - JOUR ID - 1360245 AU - Mlynáriková, Katarína - Šedo, Ondrej - Růžička, Filip - Zdráhal, Zbyněk - Holá, Veronika - Mahelová, Martina PY - 2016 TI - Evaluation of capacity to detect ability to form biofilm in Candida parapsilosis sensu stricto strains by MALDI-TOF MS JF - Folia Microbiologica VL - 61 IS - 6 SP - 465-471 EP - 465-471 PB - Springer SN - 00155632 KW - FLIGHT MASS-SPECTROMETRY KW - DESORPTION IONIZATION-TIME KW - STAPHYLOCOCCUS-EPIDERMIDIS KW - RAMAN-SPECTROSCOPY KW - SURFACES KW - BACTERIA KW - IDENTIFICATION KW - ALBICANS KW - EXPRESSION KW - DATABASE N2 - Matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is, currently, used as a rapid and reliable tool in microbial diagnostics. The discriminatory power of the method extends its applicability also beyond species level. This study examined the possibility to use MALDI-TOF MS to differentiate between Candida parapsilosis sensu stricto biofilm-positive (n = 12) and biofilm-negative (n = 9) strains. The results indicated a grouping trend within MALDI-TOF mass spectra belonging to each of the tested groups. However, these trends were eclipsed by mass spectral variations resulting from limited repeatability of the method, making its application for the selected purpose impossible. Improvement in the discriminatory power of the method was not obtained neither by using different matrices (alpha-cyano-4-hydroxycinnamic acid, ferulic acid, 5-chloro-2-mercaptobenzothionazole) for MALDI-TOF MS analysis nor by testing different culture conditions (cultivation length, culture media). ER -
MLYNÁRIKOVÁ, Katarína, Ondrej ŠEDO, Filip RŮŽIČKA, Zbyněk ZDRÁHAL, Veronika HOLÁ and Martina MAHELOVÁ. Evaluation of capacity to detect ability to form biofilm in Candida parapsilosis sensu stricto strains by MALDI-TOF MS. \textit{Folia Microbiologica}. Dordrecht: Springer, 2016, vol.~61, No~6, p.~465-471. ISSN~0015-5632. Available from: https://dx.doi.org/10.1007/s12223-016-0458-7.
|