KOMINKOVA, Marketa, Vedran MILOSAVLJEVIC, Petr VITEK, Hana POLANSKÁ, Kristyna CIHALOVA, Simona DOSTALOVA, Veronika HYNSTOVA, Roman GURÁŇ, Pavel KOPEL, Lukas RICHTERA, Michal MASAŘÍK, Martin BRTNICKY, Jindrich KYNICKY, Ondrej ZITKA and Vojtech ADAM. Comparative study on toxicity of extracellularly biosynthesized and laboratory synthesized CdTe quantum dots. Online. Journal of Biotechnology. Amsterdam: Elsevier Science BV, 2017, vol. 241, "neuvedeno", p. 193-200. ISSN 0168-1656. Available from: https://dx.doi.org/10.1016/j.jbiotec.2016.10.024. [citováno 2024-04-23]
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Basic information
Original name Comparative study on toxicity of extracellularly biosynthesized and laboratory synthesized CdTe quantum dots
Authors KOMINKOVA, Marketa (203 Czech Republic), Vedran MILOSAVLJEVIC (203 Czech Republic), Petr VITEK (203 Czech Republic), Hana POLANSKÁ (203 Czech Republic, belonging to the institution), Kristyna CIHALOVA (203 Czech Republic), Simona DOSTALOVA (203 Czech Republic), Veronika HYNSTOVA (203 Czech Republic), Roman GURÁŇ (203 Czech Republic), Pavel KOPEL (203 Czech Republic), Lukas RICHTERA (203 Czech Republic), Michal MASAŘÍK (203 Czech Republic, guarantor, belonging to the institution), Martin BRTNICKY (203 Czech Republic), Jindrich KYNICKY (203 Czech Republic), Ondrej ZITKA (203 Czech Republic) and Vojtech ADAM (203 Czech Republic)
Edition Journal of Biotechnology, Amsterdam, Elsevier Science BV, 2017, 0168-1656.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30400 3.4 Medical biotechnology
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 2.533
RIV identification code RIV/00216224:14110/17:00095934
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.1016/j.jbiotec.2016.10.024
UT WoS 000392677900026
Keywords in English Quantum dots; Biosynthesis; Escherichia coli (E. coli); CdTe; Toxicity
Tags EL OK
Tags International impact, Reviewed
Changed by Changed by: Soňa Böhmová, učo 232884. Changed: 20/3/2018 18:13.
Abstract
Nanobiosynthesis belongs to the most recent methods for synthesis of nanoparticles. This type of synthesis provides many advantages including the uniformity in particle shape and size. The biosynthesis has also a significant advantage regarding chemical properties of the obtained particles. In this study, we characterized the basic properties and composition of quantum dots (QDs), obtained by the extracellular biosynthesis by Escherichia coli. Furthermore, the toxicity of the biosynthesized QDs was compared to QDs prepared by microwave synthesis. The obtained results revealed the presence of cyan CdTe QDs after removal of substantial amounts of organic compounds, which stabilized the nanoparticle surface. QDs toxicity was evaluated using three cell lines Human Foreskin Fibroblast (HFF), Human Prostate Cancer cells (PC-3) and Breast Cancer cells (MCF-7) and the MTT assay. The test revealed differences in the toxicity between variants of QDs, varying about 10% in the HFF and 30% in the MCF-7 cell lines. The toxicity of the biosynthesized QDs to the PC-3 cell lines was about 35% lower in comparison with the QDs prepared by microwave synthesis.
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