Plant alkaloid sanguinarine and novel potential probiotic
strains Lactobacillus apis, Lactobacillus melliventris and
Gilliamella apicola promote resistance of honey bees to
nematobacterial infection

J 2017

Plant alkaloid sanguinarine and novel potential probiotic strains Lactobacillus apis, Lactobacillus melliventris and Gilliamella apicola promote resistance of honey bees to nematobacterial infection

HYRŠL, Pavel, Pavel DOBEŠ, Libor VOJTEK, Zuzana HRONCOVÁ, Jan TYL et. al.

Basic information

Original name

Plant alkaloid sanguinarine and novel potential probiotic strains Lactobacillus apis, Lactobacillus melliventris and Gilliamella apicola promote resistance of honey bees to nematobacterial infection

Name in Czech

Plant alkaloid sanguinarine and novel potential probiotic strains Lactobacillus apis, Lactobacillus melliventris and Gilliamella apicola promote resistance of honey bees to nematobacterial infection

Authors

HYRŠL, Pavel (203 Czech Republic, guarantor, belonging to the institution), Pavel DOBEŠ (203 Czech Republic, belonging to the institution), Libor VOJTEK (203 Czech Republic, belonging to the institution), Zuzana HRONCOVÁ (203 Czech Republic), Jan TYL (203 Czech Republic) and Jiří KILLER (203 Czech Republic)

Edition

Bulletin of Insectology, Bologna, ALMA MATER STUDIORUM, UNIV BOLOGNA, 2017, 1721-8861

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30102 Immunology

Country of publisher

Italy

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.088

RIV identification code

RIV/00216224:14310/17:00108323

Organization unit

Faculty of Science

UT WoS

000399955300005

Keywords (in Czech)

entomopathogenní hlístice; včela medonosná; imunita; nepatogenní bakterie; rostlinný alkaloid

Keywords in English

entomopathogenic nematode; honey bee; immunity; non-pathogenic bacteria; plant alkaloid

Abstract

V originále

Entomopathogenic nematodes (EPNs) are obligate insect parasites symbiotically associated with entomopathogenic bacteria. They can be used as a natural infection model combining bacterial infection with infection by multicellular parasite and as such, can be used in biological control and also offer a powerful tool to study insect immunity. Both mix of non-pathogenic bacteria and even nectar alkaloids were previously hypothesized to positively modulate honey bee health. We used EPNs for evaluating the overall immune resistance of honey bee larvae treated with potentially immuno-modulating substances - a plant alkaloid sanguinarine (extracted from Macleaya cordata) and non-pathogenic strains of Lactobacillus apis, Lactobacillus melliventris and Gilliamella apicola, native to honey bee gut isolates. Honey bee 5th instar larvae (L5) and white pupae were infected with Heterorhabditis bacteriophora or Steinernema feltiae, both carrying their symbiotic bacteria. In comparison to untreated honey bee larvae we ob-served an increase in survival of 13.5 ± 6.43 or 11.25 ± 5.77% in case of sanguinarine and S. feltiae or H. bacteriophora, respec-tively. Similarly, mix of above mentioned bacteria inoculated at the same time increased survivorship to 23.25 ± 1.53 or 11.0 ± 6.0% for S. feltiae or H. bacteriophora. This is the first record of use of nematobacterial infection for evaluating the immune status of a beneficial insect. Addition of low doses of both sanguinarine and non-pathogenic strains of selected bacteria had a positive impact on the resistance of honey bee to pathogen. This method can serve as a valuable tool for immunological tests in honey bees.

Links

QJ1210047, research and development project

Name: Vývoj nových prostředků pro podporu imunity včel, prevenci a léčbu včelích onemocnění (Acronym: Probiotika - imunita včel)

Investor: Ministry of Agriculture of the CR

Citovat

HYRŠL, Pavel, Pavel DOBEŠ, Libor VOJTEK, Zuzana HRONCOVÁ, Jan TYL and Jiří KILLER. Plant alkaloid sanguinarine and novel potential probiotic strains Lactobacillus apis, Lactobacillus melliventris and Gilliamella apicola promote resistance of honey bees to nematobacterial infection. Bulletin of Insectology. Bologna: ALMA MATER STUDIORUM, UNIV BOLOGNA, 2017, vol. 70, No 1, p. 31-38. ISSN 1721-8861.

@article{1363167,
   author = {Hyršl, Pavel and Dobeš, Pavel and Vojtek, Libor and Hroncová, Zuzana and Tyl, Jan and Killer, Jiří},
   article_location = {Bologna},
   article_number = {1},
   keywords = {entomopathogenic nematode; honey bee; immunity; non-pathogenic bacteria; plant alkaloid},
   language = {eng},
   issn = {1721-8861},
   journal = {Bulletin of Insectology},
   title = {Plant alkaloid sanguinarine and novel potential probiotic strains Lactobacillus apis, Lactobacillus melliventris and Gilliamella apicola promote resistance of honey bees to nematobacterial infection},
   url = {http://www.bulletinofinsectology.org/pdfarticles/vol70-2017-031-038hyrsl.pdf},
   volume = {70},
   year = {2017}
}

TY  - JOUR
ID  - 1363167
AU  - Hyršl, Pavel - Dobeš, Pavel - Vojtek, Libor - Hroncová, Zuzana - Tyl, Jan - Killer, Jiří
PY  - 2017
TI  - Plant alkaloid sanguinarine and novel potential probiotic strains Lactobacillus apis, Lactobacillus melliventris and Gilliamella apicola promote resistance of honey bees to nematobacterial infection
JF  - Bulletin of Insectology
VL  - 70
IS  - 1
SP  - 31-38
EP  - 31-38
PB  - ALMA MATER STUDIORUM, UNIV BOLOGNA
SN  - 17218861
KW  - entomopathogenic nematode
KW  - honey bee
KW  - immunity
KW  - non-pathogenic bacteria
KW  - plant alkaloid
UR  - http://www.bulletinofinsectology.org/pdfarticles/vol70-2017-031-038hyrsl.pdf
L2  - http://www.bulletinofinsectology.org/pdfarticles/vol70-2017-031-038hyrsl.pdf
N2  - Entomopathogenic nematodes (EPNs) are obligate insect parasites symbiotically associated with entomopathogenic bacteria. They can be used as a natural infection model combining bacterial infection with infection by multicellular parasite and as such, can be used in biological control and also offer a powerful tool to study insect immunity. Both mix of non-pathogenic bacteria and even nectar alkaloids were previously hypothesized to positively modulate honey bee health. We used EPNs for evaluating the overall immune resistance of honey bee larvae treated with potentially immuno-modulating substances - a plant alkaloid sanguinarine (extracted from Macleaya cordata) and non-pathogenic strains of Lactobacillus apis, Lactobacillus melliventris and Gilliamella apicola, native to honey bee gut isolates. Honey bee 5th instar larvae (L5) and white pupae were infected with Heterorhabditis bacteriophora or Steinernema feltiae, both carrying their symbiotic bacteria. In comparison to untreated honey bee larvae we ob-served an increase in survival of 13.5 ± 6.43 or 11.25 ± 5.77% in case of sanguinarine and S. feltiae or H. bacteriophora, respec-tively. Similarly, mix of above mentioned bacteria inoculated at the same time increased survivorship to 23.25 ± 1.53 or 11.0 ± 6.0% for S. feltiae or H. bacteriophora. This is the first record of use of nematobacterial infection for evaluating the immune status of a beneficial insect. Addition of low doses of both sanguinarine and non-pathogenic strains of selected bacteria had a positive impact on the resistance of honey bee to pathogen. This method can serve as a valuable tool for immunological tests in honey bees.
ER  -

HYRŠL, Pavel, Pavel DOBEŠ, Libor VOJTEK, Zuzana HRONCOVÁ, Jan TYL and Jiří KILLER. Plant alkaloid sanguinarine and novel potential probiotic strains Lactobacillus apis, Lactobacillus melliventris and Gilliamella apicola promote resistance of honey bees to nematobacterial infection. \textit{Bulletin of Insectology}. Bologna: ALMA MATER STUDIORUM, UNIV BOLOGNA, 2017, vol.~70, No~1, p.~31-38. ISSN~1721-8861.

Detailed Information on Publication Record