Glucose determination in fish plasma by two different moderate methods

BARTOŇKOVÁ, Jana, Pavel HYRŠL and Libor VOJTEK. Glucose determination in fish plasma by two different moderate methods. Acta Veterinaria. Brno: VETERINARNI A FARMACEUTICKA UNIVERZITA B, 2016, vol. 85, No 4, p. 349-353. ISSN 0001-7213. Available from: https://dx.doi.org/10.2754/avb201685040349.

Basic information

• Original name: Glucose determination in fish plasma by two different moderate methods
• Authors: BARTOŇKOVÁ, Jana (203 Czech Republic, belonging to the institution), Pavel HYRŠL (203 Czech Republic, belonging to the institution) and Libor VOJTEK (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Acta Veterinaria, Brno, VETERINARNI A FARMACEUTICKA UNIVERZITA B, 2016, 0001-7213.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 30105 Physiology
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 0.415
• RIV identification code: RIV/00216224:14310/16:00092118
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.2754/avb201685040349
• UT WoS: 000398194600005
• Keywords (in Czech): glukometr; spectrofotometrie; lín; Tinca tinca
• Keywords in English: glucometer; spectrophotometry; tench; Tinca tinca
• Tags: AKR, rivok
• Tags: International impact, Reviewed

Abstract

Group of teleost fish consist from many different species and has large economic, societal and environmental impact. Glucose is the main source of energy for most vertebrate organisms. Fish as well as for example mammals maintain specific level of blood sugar – glycaemia, which is influenced by multiple factors such as hormone production, diet and temperature. Therefore, it is favourable parameter of scientists to check the immediate state of an organism. Most utilized method to measure glycaemia in farmed fish is spectrophotometry, however for field experiments is this method often inconvenient. Here we report that basic spectrophotometry using oxidation of blood beta D-glucose by glucose oxidase (used in laboratory conditions) can be easily substituted with portable glucometer measurements, primarily designed for human samples. This is very useful thanks to the possibility of use outside the laboratory and its small sample volume demands. Terrain studies often require fast sample processing and conditions of measurement are limited. Using glucometer is very simple and elegant option. We have tested 74 blood plasma samples (29 males, 45 females) of tench (Tinca tinca) both spectrophotometrically and using human glucometer. Our results show significant linear correlation (p < 0.001) between both methods. The main contribution of this study consists in comparability and interchangeability of results obtained from both presented methods.