Glucose determination in fish plasma by two different moderate
methods

J 2016

Glucose determination in fish plasma by two different moderate methods

BARTOŇKOVÁ, Jana, Pavel HYRŠL and Libor VOJTEK

Basic information

Original name

Glucose determination in fish plasma by two different moderate methods

Authors

BARTOŇKOVÁ, Jana (203 Czech Republic, belonging to the institution), Pavel HYRŠL (203 Czech Republic, belonging to the institution) and Libor VOJTEK (203 Czech Republic, guarantor, belonging to the institution)

Edition

Acta Veterinaria, Brno, VETERINARNI A FARMACEUTICKA UNIVERZITA B, 2016, 0001-7213

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30105 Physiology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 0.415

RIV identification code

RIV/00216224:14310/16:00092118

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.2754/avb201685040349

UT WoS

000398194600005

Keywords (in Czech)

glukometr; spectrofotometrie; lín; Tinca tinca

Keywords in English

glucometer; spectrophotometry; tench; Tinca tinca

Abstract

V originále

Group of teleost fish consist from many different species and has large economic, societal and environmental impact. Glucose is the main source of energy for most vertebrate organisms. Fish as well as for example mammals maintain specific level of blood sugar – glycaemia, which is influenced by multiple factors such as hormone production, diet and temperature. Therefore, it is favourable parameter of scientists to check the immediate state of an organism. Most utilized method to measure glycaemia in farmed fish is spectrophotometry, however for field experiments is this method often inconvenient. Here we report that basic spectrophotometry using oxidation of blood beta D-glucose by glucose oxidase (used in laboratory conditions) can be easily substituted with portable glucometer measurements, primarily designed for human samples. This is very useful thanks to the possibility of use outside the laboratory and its small sample volume demands. Terrain studies often require fast sample processing and conditions of measurement are limited. Using glucometer is very simple and elegant option. We have tested 74 blood plasma samples (29 males, 45 females) of tench (Tinca tinca) both spectrophotometrically and using human glucometer. Our results show significant linear correlation (p < 0.001) between both methods. The main contribution of this study consists in comparability and interchangeability of results obtained from both presented methods.

Citovat

BARTOŇKOVÁ, Jana, Pavel HYRŠL and Libor VOJTEK. Glucose determination in fish plasma by two different moderate methods. Acta Veterinaria. Brno: VETERINARNI A FARMACEUTICKA UNIVERZITA B, 2016, vol. 85, No 4, p. 349-353. ISSN 0001-7213. Available from: https://dx.doi.org/10.2754/avb201685040349.

@article{1363171,
   author = {Bartoňková, Jana and Hyršl, Pavel and Vojtek, Libor},
   article_location = {Brno},
   article_number = {4},
   doi = {http://dx.doi.org/10.2754/avb201685040349},
   keywords = {glucometer; spectrophotometry; tench; Tinca tinca},
   language = {eng},
   issn = {0001-7213},
   journal = {Acta Veterinaria},
   title = {Glucose determination in fish plasma by two different moderate methods},
   url = {http://dx.doi.org/10.2754/avb201685040349},
   volume = {85},
   year = {2016}
}

TY  - JOUR
ID  - 1363171
AU  - Bartoňková, Jana - Hyršl, Pavel - Vojtek, Libor
PY  - 2016
TI  - Glucose determination in fish plasma by two different moderate methods
JF  - Acta Veterinaria
VL  - 85
IS  - 4
SP  - 349-353
EP  - 349-353
PB  - VETERINARNI A FARMACEUTICKA UNIVERZITA B
SN  - 00017213
KW  - glucometer
KW  - spectrophotometry
KW  - tench
KW  - Tinca tinca
UR  - http://dx.doi.org/10.2754/avb201685040349
L2  - http://dx.doi.org/10.2754/avb201685040349
N2  - Group of teleost fish consist from many different species and has large economic, societal and environmental impact. Glucose is the main source of energy for most vertebrate organisms. Fish as well as for example mammals maintain specific level of blood sugar – glycaemia, which is influenced by multiple factors such as hormone production, diet and temperature. Therefore, it is favourable parameter of scientists to check the immediate state of an organism. Most utilized method to measure glycaemia in farmed fish is spectrophotometry, however for field experiments is this method often inconvenient. Here we report that basic spectrophotometry using oxidation of blood beta D-glucose by glucose oxidase (used in laboratory conditions) can be easily substituted with portable glucometer measurements, primarily designed for human samples. This is very useful thanks to the possibility of use outside the laboratory and its small sample volume demands. Terrain studies often require fast sample processing and conditions of measurement are limited. Using glucometer is very simple and elegant option. We have tested 74 blood plasma samples (29 males, 45 females) of tench (Tinca tinca) both spectrophotometrically and using human glucometer. Our results show significant linear correlation (p < 0.001) between both methods. The main contribution of this study consists in comparability and interchangeability of results obtained from both presented methods.
ER  -

BARTOŇKOVÁ, Jana, Pavel HYRŠL and Libor VOJTEK. Glucose determination in fish plasma by two different moderate methods. \textit{Acta Veterinaria}. Brno: VETERINARNI A FARMACEUTICKA UNIVERZITA B, 2016, vol.~85, No~4, p.~349-353. ISSN~0001-7213. Available from: https://dx.doi.org/10.2754/avb201685040349.

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