Simultaneous Determination of Uric Acid, Xanthine and
Hypoxanthine in Human Plasma and Serum by HPLC–UV: Uric Acid
Metabolism Tracking

J 2017

Simultaneous Determination of Uric Acid, Xanthine and Hypoxanthine in Human Plasma and Serum by HPLC–UV: Uric Acid Metabolism Tracking

PLESKAČOVÁ, Anna, Stanislav BREJCHA, Lukáš PÁCAL, Kateřina KAŇKOVÁ, Josef TOMANDL et. al.

Basic information

Original name

Simultaneous Determination of Uric Acid, Xanthine and Hypoxanthine in Human Plasma and Serum by HPLC–UV: Uric Acid Metabolism Tracking

Authors

PLESKAČOVÁ, Anna (203 Czech Republic, belonging to the institution), Stanislav BREJCHA (203 Czech Republic, belonging to the institution), Lukáš PÁCAL (203 Czech Republic, belonging to the institution), Kateřina KAŇKOVÁ (203 Czech Republic, belonging to the institution) and Josef TOMANDL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Chromatographia, Heidelberg, Springer, 2017, 0009-5893

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.401

RIV identification code

RIV/00216224:14110/17:00095624

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1007/s10337-016-3208-8

UT WoS

000399024500003

Keywords in English

HPLC; Uric; acid; Xanthine; Hypoxanthine; Plasma; Serum

Abstract

V originále

A simple reversed phase HPLC method with UV detection in isocratic conditions was developed and validated for the simultaneous determination of hypoxanthine, xanthine and uric acid levels in human plasma and serum. One analysis run takes 6.5 min including a short organic mobile phase gradient for column regeneration. Concentrations of uric acid, xanthine and hypoxanthine in plasma and serum samples were highly comparable. However, hypoxanthine levels were increased in serum compared to plasma samples due to a prolonged time between serum and blood elements separation. The method was validated for linearity, precision, accuracy, sensitivity and robustness in a similar manner to that for pharmacokinetic data and it is appropriate for physiological and pathophysiological levels of all analytes. The stability of stock standard solutions was verified using spectrophotometric analysis in different conditions. The method is simple and robust with a good precision for the measurement of hypoxanthine, xanthine and uric acid in human plasma and serum.

Links

MUNI/A/1056/2015, interní kód MU

Name: Příspěvek chemických a biochemických metodik ke studiu molekulární podstaty vybraných patologických stavů a onemocnění (Acronym: bichepat)

Investor: Masaryk University, Category A

NV16-28040A, research and development project

Name: Dlouhodobé dopady gestačního diabetes mellitus pro metabolické zdraví žen časně postpartum: význam nových diagnostických kritérií

Citovat

PLESKAČOVÁ, Anna, Stanislav BREJCHA, Lukáš PÁCAL, Kateřina KAŇKOVÁ and Josef TOMANDL. Simultaneous Determination of Uric Acid, Xanthine and Hypoxanthine in Human Plasma and Serum by HPLC–UV: Uric Acid Metabolism Tracking. Chromatographia. Heidelberg: Springer, 2017, vol. 80, No 4, p. 529-536. ISSN 0009-5893. Available from: https://dx.doi.org/10.1007/s10337-016-3208-8.

@article{1363208,
   author = {Pleskačová, Anna and Brejcha, Stanislav and Pácal, Lukáš and Kaňková, Kateřina and Tomandl, Josef},
   article_location = {Heidelberg},
   article_number = {4},
   doi = {http://dx.doi.org/10.1007/s10337-016-3208-8},
   keywords = {HPLC; Uric; acid; Xanthine; Hypoxanthine; Plasma; Serum},
   language = {eng},
   issn = {0009-5893},
   journal = {Chromatographia},
   title = {Simultaneous Determination of Uric Acid, Xanthine and Hypoxanthine in Human Plasma and Serum by HPLC–UV: Uric Acid Metabolism Tracking},
   volume = {80},
   year = {2017}
}

TY  - JOUR
ID  - 1363208
AU  - Pleskačová, Anna - Brejcha, Stanislav - Pácal, Lukáš - Kaňková, Kateřina - Tomandl, Josef
PY  - 2017
TI  - Simultaneous Determination of Uric Acid, Xanthine and Hypoxanthine in Human Plasma and Serum by HPLC–UV: Uric Acid Metabolism Tracking
JF  - Chromatographia
VL  - 80
IS  - 4
SP  - 529-536
EP  - 529-536
PB  - Springer
SN  - 00095893
KW  - HPLC
KW  - Uric
KW  - acid
KW  - Xanthine
KW  - Hypoxanthine
KW  - Plasma
KW  - Serum
N2  - A simple reversed phase HPLC method with UV detection in isocratic conditions was developed and validated for the simultaneous determination of hypoxanthine, xanthine and uric acid levels in human plasma and serum. One analysis run takes 6.5 min including a short organic mobile phase gradient for column regeneration. Concentrations of uric acid, xanthine and hypoxanthine in plasma and serum samples were highly comparable. However, hypoxanthine levels were increased in serum compared to plasma samples due to a prolonged time between serum and blood elements separation. The method was validated for linearity, precision, accuracy, sensitivity and robustness in a similar manner to that for pharmacokinetic data and it is appropriate for physiological and pathophysiological levels of all analytes. The stability of stock standard solutions was verified using spectrophotometric analysis in different conditions. The method is simple and robust with a good precision for the measurement of hypoxanthine, xanthine and uric acid in human plasma and serum.
ER  -

PLESKAČOVÁ, Anna, Stanislav BREJCHA, Lukáš PÁCAL, Kateřina KAŇKOVÁ and Josef TOMANDL. Simultaneous Determination of Uric Acid, Xanthine and Hypoxanthine in Human Plasma and Serum by HPLC–UV: Uric Acid Metabolism Tracking. \textit{Chromatographia}. Heidelberg: Springer, 2017, vol.~80, No~4, p.~529-536. ISSN~0009-5893. Available from: https://dx.doi.org/10.1007/s10337-016-3208-8.

Detailed Information on Publication Record