| MLČOCHOVÁ, Hana, Táňa MACHÁČKOVÁ, Anja RABIEN, Lenka RADOVÁ, Pavel FABIAN, Robert ILIEV, Kateřina SLABÁ, Alexandr POPRACH, Ergin KILIC, Michal STANIK, Martina LOJOVÁ, Marek SVOBODA, Jan DOLEZEL, Rostislav VYZULA, Klaus JUNG and Ondřej SLABÝ. Epithelial-mesenchymal transition-associated microRNA/mRNA signature is linked to metastasis and prognosis in clear-cell renal cell carcinoma. Scientific Reports. LONDON: NATURE PUBLISHING GROUP, 2016, vol. 6, August, p. nestránkováno, 12 pp. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/srep31852. |
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@article{1363865,
author = {Mlčochová, Hana and Macháčková, Táňa and Rabien, Anja and Radová, Lenka and Fabian, Pavel and Iliev, Robert and Slabá, Kateřina and Poprach, Alexandr and Kilic, Ergin and Stanik, Michal and Lojová, Martina and Svoboda, Marek and Dolezel, Jan and Vyzula, Rostislav and Jung, Klaus and Slabý, Ondřej},
article_location = {LONDON},
article_number = {August},
doi = {http://dx.doi.org/10.1038/srep31852},
keywords = {CANCER; GENE; EXPRESSION; PATHWAYS; TARGETS; PROTEIN; FREM1},
language = {eng},
issn = {2045-2322},
journal = {Scientific Reports},
title = {Epithelial-mesenchymal transition-associated microRNA/mRNA signature is linked to metastasis and prognosis in clear-cell renal cell carcinoma},
url = {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4994011/pdf/srep31852.pdf},
volume = {6},
year = {2016}
}
TY - JOUR
ID - 1363865
AU - Mlčochová, Hana - Macháčková, Táňa - Rabien, Anja - Radová, Lenka - Fabian, Pavel - Iliev, Robert - Slabá, Kateřina - Poprach, Alexandr - Kilic, Ergin - Stanik, Michal - Lojová, Martina - Svoboda, Marek - Dolezel, Jan - Vyzula, Rostislav - Jung, Klaus - Slabý, Ondřej
PY - 2016
TI - Epithelial-mesenchymal transition-associated microRNA/mRNA signature is linked to metastasis and prognosis in clear-cell renal cell carcinoma
JF - Scientific Reports
VL - 6
IS - August
SP - nestránkováno
EP - nestránkováno
PB - NATURE PUBLISHING GROUP
SN - 20452322
KW - CANCER
KW - GENE
KW - EXPRESSION
KW - PATHWAYS
KW - TARGETS
KW - PROTEIN
KW - FREM1
UR - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4994011/pdf/srep31852.pdf
L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4994011/pdf/srep31852.pdf
N2 - Clear-cell renal cell carcinomas (ccRCCs) are genetically heterogeneous tumors presenting diverse clinical courses. Epithelial-mesenchymal transition (EMT) is a crucial process involved in initiation of metastatic cascade. The aim of our study was to identify an integrated miRNA/mRNA signature associated with metastasis and prognosis in ccRCC through targeted approach based on analysis of miRNAs/mRNAs associated with EMT. A cohort of 230 ccRCC was included in our study and further divided into discovery, training and validation cohorts. EMT markers were evaluated in ccRCC tumor samples, which were grouped accordingly to EMT status. By use of large-scale miRNA/mRNA expression profiling, we identified miRNA/mRNA with significantly different expression in EMT-positive tumors and selected 41 miRNAs/mRNAs for training phase of the study to evaluate their diagnostic and prognostic potential. Fifteen miRNAs/mRNAs were analyzed in the validation phase, where all evaluated miRNA/mRNA candidates were confirmed to be significantly deregulated in tumor tissue. Some of them significantly differed in metastatic tumors, correlated with clinical stage, with Fuhrman grade and with overall survival. Further, we established an EMT-based stage-independent prognostic scoring system enabling identification of ccRCC patients at high-risk of cancer-related death. Finally, we confirmed involvement of miR-429 in EMT regulation in RCC cells in vitro.
ER -
MLČOCHOVÁ, Hana, Táňa MACHÁČKOVÁ, Anja RABIEN, Lenka RADOVÁ, Pavel FABIAN, Robert ILIEV, Kateřina SLABÁ, Alexandr POPRACH, Ergin KILIC, Michal STANIK, Martina LOJOVÁ, Marek SVOBODA, Jan DOLEZEL, Rostislav VYZULA, Klaus JUNG and Ondřej SLABÝ. Epithelial-mesenchymal transition-associated microRNA/mRNA signature is linked to metastasis and prognosis in clear-cell renal cell carcinoma. \textit{Scientific Reports}. LONDON: NATURE PUBLISHING GROUP, 2016, vol.~6, August, p.~nestránkováno, 12 pp. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/srep31852.
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