Development of an RP-HPLC method for determination of salicylic
acid and its metabolic intermediates from plant biosynthesis
pathways

D 2016

Development of an RP-HPLC method for determination of salicylic acid and its metabolic intermediates from plant biosynthesis pathways

KOŽUSZNIKOVÁ, Eliška; Miroslava BITTOVÁ a Petr KUBÁŇ

Základní údaje

Originální název

Development of an RP-HPLC method for determination of salicylic acid and its metabolic intermediates from plant biosynthesis pathways

Autoři

KOŽUSZNIKOVÁ, Eliška; Miroslava BITTOVÁ a Petr KUBÁŇ

Vydání

Olomouc, Advances in chromatography and electrophoresis & Chiranal 2016, od s. 112-113, 2 s. 2016

Nakladatel

Palacký University, Olomouc

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10406 Analytical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/16:00092194

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-244-4961-6

Klíčová slova anglicky

RP-HPLC; method validation; salicylic acid; gentisic acid; salicylaldehyde; saligenin

Změněno: 7. 12. 2016 14:07, Mgr. Miroslava Bittová, Ph.D.

Anotace

V originále

Salicylic acid (2-hydroxybenzoic acid, SA) belongs to a group of plant growth regulators that have an aromatic ring bearing a hydroxyl group or its functional derivative in the structure. SA functions as an important plant signaling compound that is synthesized in plants as a response to various pathogenic attacks or environmental stress (caused by microbes, insects, herbivores etc.). Two pathways of SA biosynthesis were described in the literature, but the production of SA is still not fully clarified. To better understand the various transformation pathways of SA, analytical methods capable of separating not only SA, but also other relevant compounds from the biotransformation cycles need to be developed. In this study, a reverse-phase high performance liquid chromatography (RP-HPLC) method for determination of SA and metabolic intermediates derived from its biosynthetic pathways (salicin (2-(hydroxymethyl)phenyl-beta-D-glucopyranoside), helicin (salicylaldehyde beta-D-glucoside), gentisic acid (2,5-dihydroxybenzoic acid), saligenin (2-hydroxybenzyl alcohol) and salicylaldehyde (2-hydroxybenzaldehyde) was developed. The method was then applied for analysis of samples from white willow (Salix alba), as willow cortex is well-known as a natural source of salicin and salicylic acid.

Návaznosti

MUNI/A/1452/2014, interní kód MU

Název: Vývoj metod a instrumentace pro analýzu biologicky významných látek 2015

Investor: Masarykova univerzita, Vývoj metod a instrumentace pro analýzu biologicky významných látek 2015, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

KOŽUSZNIKOVÁ, Eliška; Miroslava BITTOVÁ a Petr KUBÁŇ. Development of an RP-HPLC method for determination of salicylic acid and its metabolic intermediates from plant biosynthesis pathways. In Vítězslav Maier. Advances in chromatography and electrophoresis & Chiranal 2016. Olomouc: Palacký University, Olomouc, 2016, s. 112-113. ISBN 978-80-244-4961-6.

@inproceedings{1363919,
   author = {Kožuszniková, Eliška and Bittová, Miroslava and Kubáň, Petr},
   address = {Olomouc},
   booktitle = {Advances in chromatography and electrophoresis & Chiranal 2016},
   editor = {Vítězslav Maier},
   keywords = {RP-HPLC; method validation; salicylic acid; gentisic acid; salicylaldehyde; saligenin},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {Olomouc},
   isbn = {978-80-244-4961-6},
   pages = {112-113},
   publisher = {Palacký University, Olomouc},
   title = {Development of an RP-HPLC method for determination of salicylic acid and its metabolic intermediates from plant biosynthesis pathways},
   year = {2016}
}

TY  - CONF
ID  - 1363919
AU  - Kožuszniková, Eliška - Bittová, Miroslava - Kubáň, Petr
PY  - 2016
TI  - Development of an RP-HPLC method for determination of salicylic acid and its metabolic intermediates from plant biosynthesis pathways
PB  - Palacký University, Olomouc
CY  - Olomouc
SN  - 9788024449616
KW  - RP-HPLC
KW  - method validation
KW  - salicylic acid
KW  - gentisic acid
KW  - salicylaldehyde
KW  - saligenin
N2  - Salicylic acid (2-hydroxybenzoic acid, SA) belongs to a group of plant growth regulators that have an aromatic ring bearing a hydroxyl group or its functional derivative in the structure. SA functions as an important plant signaling compound that is synthesized in plants as a response to various pathogenic attacks or environmental stress (caused by microbes, insects, herbivores etc.). Two pathways of SA biosynthesis were described in the literature, but the production of SA is still not fully clarified. To better understand the various transformation pathways of SA, analytical methods capable of separating not only SA, but also other relevant compounds from the biotransformation cycles need to be developed. In this study, a reverse-phase high performance liquid chromatography (RP-HPLC) method for determination of SA and metabolic intermediates derived from its biosynthetic pathways (salicin (2-(hydroxymethyl)phenyl-beta-D-glucopyranoside), helicin (salicylaldehyde beta-D-glucoside), gentisic acid (2,5-dihydroxybenzoic acid), saligenin (2-hydroxybenzyl alcohol) and salicylaldehyde (2-hydroxybenzaldehyde) was developed. The method was then applied for analysis of samples from white willow (Salix alba), as willow cortex is well-known as a natural source of salicin and salicylic acid.
ER  -

KOŽUSZNIKOVÁ, Eliška; Miroslava BITTOVÁ a Petr KUBÁŇ. Development of an RP-HPLC method for determination of salicylic acid and its metabolic intermediates from plant biosynthesis pathways. In Vítězslav Maier. \textit{Advances in chromatography and electrophoresis \&{} Chiranal 2016}. Olomouc: Palacký University, Olomouc, 2016, s.~112-113. ISBN~978-80-244-4961-6.

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