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@inproceedings{1364184,
author = {Cmiel, Vratislav and Gumulec, Jaromír and Svoboda, Ondrej and Raudenská, Martina and Hudcová, Kristýna and Sekora, Jiri and Balogh, Jaroslav and Masařík, Michal and Provaznik, Ivo},
address = {Bellingham},
booktitle = {Proceedings of SPIE : Optical Diagnostics and Sensing XVI - Toward Point-of-Care Diagnostics},
doi = {http://dx.doi.org/10.1117/12.2213253},
editor = {Gerard L. Coté},
keywords = {mRNA; smartphone; PCR; cancer; metallothionein},
howpublished = {tištěná verze "print"},
language = {eng},
location = {Bellingham},
isbn = {978-1-62841-949-8},
pages = {1-6},
publisher = {SPIE},
title = {All-in-one detector of circulating mRNA based on a smartphone},
year = {2016}
}
TY - JOUR
ID - 1364184
AU - Cmiel, Vratislav - Gumulec, Jaromír - Svoboda, Ondrej - Raudenská, Martina - Hudcová, Kristýna - Sekora, Jiri - Balogh, Jaroslav - Masařík, Michal - Provaznik, Ivo
PY - 2016
TI - All-in-one detector of circulating mRNA based on a smartphone
PB - SPIE
CY - Bellingham
SN - 9781628419498
KW - mRNA
KW - smartphone
KW - PCR
KW - cancer
KW - metallothionein
N2 - Metallothionein is significantly elevated in various tumors, notably in prostate cancer on both mRNA and protein level. We demonstrated a strong predictive potential of free circulating metallothionein 2A isoform mRNA for patients with this cancer. Circulating mRNA detection relies on expensive equipment and requires high level of expertise. In this work we developed compact "all-in-one" laboratory system which replace microvolume spectrophotometer, thermocycler and realtime PCR machines. We managed to design and construct a microprocessor controlled heating/cooling chamber that ensures required temperature gradient. The chamber includes implemented optical system to enable fluorescence excitation and fluorescence analysis using a smart-phone.
ER -
CMIEL, Vratislav, Jaromír GUMULEC, Ondrej SVOBODA, Martina RAUDENSKÁ, Kristýna HUDCOVÁ, Jiri SEKORA, Jaroslav BALOGH, Michal MASAŘÍK and Ivo PROVAZNIK. All-in-one detector of circulating mRNA based on a smartphone. In Gerard L. Coté. \textit{Proceedings of SPIE : Optical Diagnostics and Sensing XVI - Toward Point-of-Care Diagnostics}. Bellingham: SPIE, 2016, p.~1-6. ISBN~978-1-62841-949-8. Available from: https://dx.doi.org/10.1117/12.2213253.
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