Detailed Information on Publication Record
2016
Absence of positive selection on CenH3 in Luzula suggests that holokinetic chromosomes may suppress centromere drive
ZEDEK, František and Petr BUREŠBasic information
Original name
Absence of positive selection on CenH3 in Luzula suggests that holokinetic chromosomes may suppress centromere drive
Authors
ZEDEK, František (203 Czech Republic, belonging to the institution) and Petr BUREŠ (203 Czech Republic, guarantor, belonging to the institution)
Edition
Annals of Botany, Oxford, Oxford University Press, 2016, 0305-7364
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10611 Plant sciences, botany
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 4.041
RIV identification code
RIV/00216224:14310/16:00088443
Organization unit
Faculty of Science
UT WoS
000399546800015
Keywords in English
centromere drive; meiotic drive; holokinetic chromosomes; holocentric chromosomes; positive selection; adaptive evolution; Centromeric histone H3; CenH3; CENP-A; holokinetic drive
Tags
Tags
International impact, Reviewed
Změněno: 6/3/2018 16:15, prof. RNDr. Petr Bureš, Ph.D.
Abstract
V originále
Background and Aims: The centromere drive theory explains diversity of eukaryotic centromeres as a consequence of the recurrent conflict between centromeric repeats and centromeric histone H3 (CenH3), in which selfish centromeres exploit meiotic asymmetry and CenH3 evolves adaptively to counterbalance deleterious consequences of driving centromeres. Accordingly, adaptively evolving CenH3 has so far been observed only in eukaryotes with asymmetric meiosis. However, if such evolution is a consequence of centromere drive, it should depend not only on meiotic asymmetry but also on monocentric or holokinetic chromosomal structure. Selective pressures acting on CenH3 have never been investigated in organisms with holokinetic meiosis despite the fact that holokinetic chromosomes have been hypothesized to suppress centromere drive. Therefore, the present study evaluates selective pressures acting on the CenH3 gene in holokinetic organisms for the first time, specifically in the representatives of the plant genus Luzula (Juncaceae), in which the kinetochore formation is not co-localized with any type of centromeric repeat. Methods: PCR, cloning and sequencing, and database searches were used to obtain coding CenH3 sequences from Luzula species. Codon substitution models were employed to infer selective regimes acting on CenH3 in Luzula. Key Results: In addition to the two previously published CenH3 sequences from L. nivea, 16 new CenH3 sequences have been isolated from 12 Luzula species. Two CenH3 isoforms in Luzula that originated by a duplication event prior to the divergence of analysed species were found. No signs of positive selection acting on CenH3 in Luzula were detected. Instead, evidence was found that selection on CenH3 of Luzula might have been relaxed. Conclusions: The results indicate that holokinetism itself may suppress centromere drive and, therefore, holokinetic chromosomes might have evolved as a defence against centromere drive.
Links
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