Paramagnetic nanoparticles in stem cell therapy

a 2016

Paramagnetic nanoparticles in stem cell therapy

ŠALINGOVÁ, Barbara, Pavel ŠIMARA, Petr SYNEK, Lenka ZAJÍČKOVÁ, Zuzana NICHTOVÁ et. al.

Basic information

Original name

Paramagnetic nanoparticles in stem cell therapy

Authors

ŠALINGOVÁ, Barbara (703 Slovakia, belonging to the institution), Pavel ŠIMARA (203 Czech Republic, belonging to the institution), Petr SYNEK (203 Czech Republic, belonging to the institution), Lenka ZAJÍČKOVÁ (203 Czech Republic, belonging to the institution), Zuzana NICHTOVÁ (703 Slovakia) and Irena KRONTORÁD KOUTNÁ (203 Czech Republic, belonging to the institution)

Edition

Bioimplantologie, 2016

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10601 Cell biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Bioimplantologie 2016 VIII. mezinárodní konference

RIV identification code

RIV/00216224:14330/16:00088444

Organization unit

Faculty of Informatics

Keywords in English

nanoparticles endothelial progenitor cells toxicity endocytosis

Abstract

V originále

In recent years metal oxide nanoparticles have become a useful tool in biomedicine. They are used as contrast agents for magnetic resonance imaging, carriers in drug delivery, compounds for hyperthermia cancer treatment or targets for cell tracking and guiding mechanisms. Even though recent advances bring stem cell therapy closer to clinical application, several issues must be addressed. The success of the healing process depends on precision targeting into the site of the injury. Nanoparticles provide a mechanism that effectively guides cells to the site of the action. Recently gamma–Fe2O3 and Fe3O4 were synthetized by microwave plasma torch. We studied the interaction and endocytosis of the nanoparticles with various cell cultures in order to optimize the process for future clinical application. We focused on cells with different stem properties, including human neonatal fibroblasts, human umbilical vein endothelial cells, adult vein endothelial cells and pluripotent stem cells. We confirmed the location of the nanoparticles by cell histology as well as electron microscopy. We assessed the effects of nanoparticles on cell viability, proliferation and metabolism by Annexin V and MTT assay. We observed differences in the accumulation of nanoparticles in cell cytoplasm and cell survival. We were able to achieve a level of saturation of NPs that produced cells capable of magnetic separation with minimal toxic effects. These properties favor the use of gamma–Fe2O3 and Fe3O4 synthetized by microwave plasma torch in stem cell therapy.

Links

GBP302/12/G157, research and development project

Name: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Czech Science Foundation

Citovat

ŠALINGOVÁ, Barbara, Pavel ŠIMARA, Petr SYNEK, Lenka ZAJÍČKOVÁ, Zuzana NICHTOVÁ and Irena KRONTORÁD KOUTNÁ. Paramagnetic nanoparticles in stem cell therapy. In Bioimplantologie. 2016.

@proceedings{1364948,
   author = {Šalingová, Barbara and Šimara, Pavel and Synek, Petr and Zajíčková, Lenka and Nichtová, Zuzana and Krontorád Koutná, Irena},
   booktitle = {Bioimplantologie},
   keywords = {nanoparticles endothelial progenitor cells toxicity endocytosis},
   language = {eng},
   title = {Paramagnetic nanoparticles in stem cell therapy},
   url = {http://www.bioimplantologie.cz/},
   year = {2016}
}

TY  - CONF
ID  - 1364948
AU  - Šalingová, Barbara - Šimara, Pavel - Synek, Petr - Zajíčková, Lenka - Nichtová, Zuzana - Krontorád Koutná, Irena
PY  - 2016
TI  - Paramagnetic nanoparticles in stem cell therapy
KW  - nanoparticles endothelial progenitor cells toxicity endocytosis
UR  - http://www.bioimplantologie.cz/
N2  - In recent years metal oxide nanoparticles have become a useful tool in biomedicine. They are used as contrast agents for magnetic resonance imaging, carriers in drug delivery, compounds for hyperthermia cancer treatment or targets for cell tracking and guiding mechanisms. Even though recent advances bring stem cell therapy closer to clinical application, several issues must be addressed. The success of the healing process depends on precision targeting into the site of the injury. Nanoparticles provide a mechanism that effectively guides cells to the site of the action. Recently gamma–Fe2O3 and Fe3O4 were synthetized by microwave plasma torch. We studied the interaction and endocytosis of the nanoparticles with various cell cultures in order to optimize the process for future clinical application. We focused on cells with different stem properties, including human neonatal fibroblasts, human umbilical vein endothelial cells, adult vein endothelial cells and pluripotent stem cells. We confirmed the location of the nanoparticles by cell histology as well as electron microscopy. We assessed the effects of nanoparticles on cell viability, proliferation and metabolism by Annexin V and MTT assay. We observed differences in the accumulation of nanoparticles in cell cytoplasm and cell survival. We were able to achieve a level of saturation of NPs that produced cells capable of magnetic separation with minimal toxic effects. These properties favor the use of gamma–Fe2O3 and Fe3O4 synthetized by microwave plasma torch in stem cell therapy.
ER  -

ŠALINGOVÁ, Barbara, Pavel ŠIMARA, Petr SYNEK, Lenka ZAJÍČKOVÁ, Zuzana NICHTOVÁ and Irena KRONTORÁD KOUTNÁ. Paramagnetic nanoparticles in stem cell therapy. In \textit{Bioimplantologie}. 2016.

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