| ROUDNICKÝ, Pavel, Jiří VOREL, Jana ILGOVÁ, Libor MIKEŠ, Lucie JEDLIČKOVÁ, John DALTON, Jan DVOŘÁK, Zbyněk ZDRÁHAL, David POTĚŠIL, Lubomír JANDA, Adam NOREK, Milan GELNAR and Martin KAŠNÝ. Eudiplozoon nipponicum (Heteronchoinea: Diplozoidae): insight into composition of E/S products and basic characterization of serpin. 2016. ISBN 978-80-210-8373-8. |
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@proceedings{1364986,
author = {Roudnický, Pavel and Vorel, Jiří and Ilgová, Jana and Mikeš, Libor and Jedličková, Lucie and Dalton, John and Dvořák, Jan and Zdráhal, Zbyněk and Potěšil, David and Janda, Lubomír and Norek, Adam and Gelnar, Milan and Kašný, Martin},
keywords = {Monogenea; Eudiplozoon; nipponicum; serpin; excretory; secretory;},
language = {eng},
isbn = {978-80-210-8373-8},
title = {Eudiplozoon nipponicum (Heteronchoinea: Diplozoidae): insight into composition of E/S products and basic characterization of serpin},
year = {2016}
}
TY - CONF
ID - 1364986
AU - Roudnický, Pavel - Vorel, Jiří - Ilgová, Jana - Mikeš, Libor - Jedličková, Lucie - Dalton, John - Dvořák, Jan - Zdráhal, Zbyněk - Potěšil, David - Janda, Lubomír - Norek, Adam - Gelnar, Milan - Kašný, Martin
PY - 2016
TI - Eudiplozoon nipponicum (Heteronchoinea: Diplozoidae): insight into composition of E/S products and basic characterization of serpin
SN - 9788021083738
KW - Monogenea
KW - Eudiplozoon
KW - nipponicum
KW - serpin
KW - excretory
KW - secretory;
N2 - Two genes coding serpins, were expressed in E. coli system (BL21 strain) in combination with synthetic genes, optimised for this system, using pET-22B(+) expression vector. IPTG induced expression (30°C, overnight) was analyzed on SDS-PAGE and soluble recombinant serpins of E. nipponicum was partially purified using Ni-NTA agarose beads. Successful production of serpins was verified by mass spectrometry and Western blot using His-tag primary antibodies.
ER -
ROUDNICKÝ, Pavel, Jiří VOREL, Jana ILGOVÁ, Libor MIKEŠ, Lucie JEDLIČKOVÁ, John DALTON, Jan DVOŘÁK, Zbyněk ZDRÁHAL, David POTĚŠIL, Lubomír JANDA, Adam NOREK, Milan GELNAR and Martin KAŠNÝ. \textit{Eudiplozoon nipponicum (Heteronchoinea: Diplozoidae): insight into composition of E/S products and basic characterization of serpin}. 2016. ISBN~978-80-210-8373-8.
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