Detailed Information on Publication Record
2016
Eudiplozoon nipponicum (Heteronchoinea: Diplozoidae): insight into composition of E/S products and basic characterization of serpin
ROUDNICKÝ, Pavel, Jiří VOREL, Jana ILGOVÁ, Libor MIKEŠ, Lucie JEDLIČKOVÁ et. al.Basic information
Original name
Eudiplozoon nipponicum (Heteronchoinea: Diplozoidae): insight into composition of E/S products and basic characterization of serpin
Authors
ROUDNICKÝ, Pavel (203 Czech Republic, guarantor, belonging to the institution), Jiří VOREL (203 Czech Republic), Jana ILGOVÁ (703 Slovakia), Libor MIKEŠ (203 Czech Republic), Lucie JEDLIČKOVÁ (203 Czech Republic), John DALTON (372 Ireland), Jan DVOŘÁK (203 Czech Republic), Zbyněk ZDRÁHAL (203 Czech Republic), David POTĚŠIL (203 Czech Republic), Lubomír JANDA (203 Czech Republic), Adam NOREK (203 Czech Republic), Milan GELNAR (203 Czech Republic) and Martin KAŠNÝ (203 Czech Republic)
Edition
2016
Other information
Language
English
Type of outcome
Prezentace na konferencích
Field of Study
10600 1.6 Biological sciences
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/16:00088448
Organization unit
Faculty of Science
ISBN
978-80-210-8373-8
Keywords in English
Monogenea; Eudiplozoon; nipponicum; serpin; excretory; secretory;
Změněno: 24/3/2017 22:28, RNDr. Martin Kašný, Ph.D.
Abstract
V originále
Two genes coding serpins, were expressed in E. coli system (BL21 strain) in combination with synthetic genes, optimised for this system, using pET-22B(+) expression vector. IPTG induced expression (30°C, overnight) was analyzed on SDS-PAGE and soluble recombinant serpins of E. nipponicum was partially purified using Ni-NTA agarose beads. Successful production of serpins was verified by mass spectrometry and Western blot using His-tag primary antibodies.
Links
| GBP505/12/G112, research and development project |
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