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@inbook{1366190,
author = {Mrázková, Jana and Malinovská, Lenka and Wimmerová, Michaela},
address = {New York},
booktitle = {In Vitro Mutagenesis Methods and Protocols},
doi = {http://dx.doi.org/10.1007/978-1-4939-6472-7},
editor = {Andrew Reeves},
keywords = {in vitro mutagenesis; site-directed mutagenesis; lectins; PA-IIL lectin},
howpublished = {tištěná verze "print"},
language = {eng},
location = {New York},
isbn = {978-1-4939-6470-3},
pages = {399-419},
publisher = {Springer Science + Business Media LLC},
title = {Step-by-Step In Vitro Mutagenesis: Lessons From Fucose-Binding Lectin PA-IIL},
year = {2017}
}
TY - CHAP
ID - 1366190
AU - Mrázková, Jana - Malinovská, Lenka - Wimmerová, Michaela
PY - 2017
TI - Step-by-Step In Vitro Mutagenesis: Lessons From Fucose-Binding Lectin PA-IIL
VL - Methods in Molecular Biology sv. 1498
PB - Springer Science + Business Media LLC
CY - New York
SN - 9781493964703
KW - in vitro mutagenesis
KW - site-directed mutagenesis
KW - lectins
KW - PA-IIL lectin
N2 - In this chapter, we describe a site-directed mutagenesis method used for investigating the sugar binding pattern of the PA-IIL lectin from the pathogenic bacterium Pseudomonas aeruginosa . Moreover, procedures for the production and purification of PA-IIL mutants are described, and several basic methods for characterizing the mutants are discussed.
ER -
MRÁZKOVÁ, Jana, Lenka MALINOVSKÁ a Michaela WIMMEROVÁ. Step-by-Step In Vitro Mutagenesis: Lessons From Fucose-Binding Lectin PA-IIL. In Andrew Reeves. \textit{In Vitro Mutagenesis Methods and Protocols}. New York: Springer Science + Business Media LLC. s.~399-419. Methods in Molecular Biology sv. 1498. ISBN~978-1-4939-6470-3. doi:10.1007/978-1-4939-6472-7. 2017.
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