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@inbook{1366190, author = {Mrázková, Jana and Malinovská, Lenka and Wimmerová, Michaela}, address = {New York}, booktitle = {In Vitro Mutagenesis Methods and Protocols}, doi = {http://dx.doi.org/10.1007/978-1-4939-6472-7}, editor = {Andrew Reeves}, keywords = {in vitro mutagenesis; site-directed mutagenesis; lectins; PA-IIL lectin}, howpublished = {tištěná verze "print"}, language = {eng}, location = {New York}, isbn = {978-1-4939-6470-3}, pages = {399-419}, publisher = {Springer Science + Business Media LLC}, title = {Step-by-Step In Vitro Mutagenesis: Lessons From Fucose-Binding Lectin PA-IIL}, year = {2017} }
TY - CHAP ID - 1366190 AU - Mrázková, Jana - Malinovská, Lenka - Wimmerová, Michaela PY - 2017 TI - Step-by-Step In Vitro Mutagenesis: Lessons From Fucose-Binding Lectin PA-IIL VL - Methods in Molecular Biology sv. 1498 PB - Springer Science + Business Media LLC CY - New York SN - 9781493964703 KW - in vitro mutagenesis KW - site-directed mutagenesis KW - lectins KW - PA-IIL lectin N2 - In this chapter, we describe a site-directed mutagenesis method used for investigating the sugar binding pattern of the PA-IIL lectin from the pathogenic bacterium Pseudomonas aeruginosa . Moreover, procedures for the production and purification of PA-IIL mutants are described, and several basic methods for characterizing the mutants are discussed. ER -
MRÁZKOVÁ, Jana, Lenka MALINOVSKÁ and Michaela WIMMEROVÁ. Step-by-Step In Vitro Mutagenesis: Lessons From Fucose-Binding Lectin PA-IIL. In Andrew Reeves. \textit{In Vitro Mutagenesis Methods and Protocols}. New York: Springer Science + Business Media LLC, 2017, p.~399-419. Methods in Molecular Biology sv. 1498. ISBN~978-1-4939-6470-3. Available from: https://dx.doi.org/10.1007/978-1-4939-6472-7.
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