Detailed Information on Publication Record
2017
Step-by-Step In Vitro Mutagenesis: Lessons From Fucose-Binding Lectin PA-IIL
MRÁZKOVÁ, Jana, Lenka MALINOVSKÁ and Michaela WIMMEROVÁBasic information
Original name
Step-by-Step In Vitro Mutagenesis: Lessons From Fucose-Binding Lectin PA-IIL
Name in Czech
Mutageneze in vitro krok za krokem: Lektin PA-IIL specifický pro fukosu
Authors
MRÁZKOVÁ, Jana (203 Czech Republic, belonging to the institution), Lenka MALINOVSKÁ (203 Czech Republic, belonging to the institution) and Michaela WIMMEROVÁ (203 Czech Republic, guarantor, belonging to the institution)
Edition
New York, In Vitro Mutagenesis Methods and Protocols, p. 399-419, 21 pp. Methods in Molecular Biology sv. 1498, 2017
Publisher
Springer Science + Business Media LLC
Other information
Language
English
Type of outcome
Kapitola resp. kapitoly v odborné knize
Field of Study
10600 1.6 Biological sciences
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
Publication form
printed version "print"
RIV identification code
RIV/00216224:14310/17:00094590
Organization unit
Faculty of Science
ISBN
978-1-4939-6470-3
UT WoS
000400922900029
Keywords (in Czech)
mutageneze in vitro; místně-cílená mutageneze; lektiny; PA-IIL lektin
Keywords in English
in vitro mutagenesis; site-directed mutagenesis; lectins; PA-IIL lectin
Tags
Tags
International impact, Reviewed
Změněno: 23/4/2020 15:31, Mgr. Marie Šípková, DiS.
V originále
In this chapter, we describe a site-directed mutagenesis method used for investigating the sugar binding pattern of the PA-IIL lectin from the pathogenic bacterium Pseudomonas aeruginosa . Moreover, procedures for the production and purification of PA-IIL mutants are described, and several basic methods for characterizing the mutants are discussed.
In Czech
V této kapitole popisujeme metodu místně-cílené mutageneze, která byla použita pro studium vazebné specifity lektinu PA-IIL, který pochází z bakterie Pseudomonas aeruginosa. Nadto, jsou zde popsány procedury pro produkci a purifikaci mutantů lektinu PA-IIL a je zde diskutováno několik základních metod pro charakterizaci těchto mutantů.
Links
| GA13-25401S, research and development project |
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| MUNI/A/1278/2016, interní kód MU |
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