A robust vitronectin-derived peptide for the scalable long-term
expansion and neuronal differentiation of human pluripotent
stem cell (hPSC)-derived neural progenitor cells (hNPCs)

J 2017

A robust vitronectin-derived peptide for the scalable long-term expansion and neuronal differentiation of human pluripotent stem cell (hPSC)-derived neural progenitor cells (hNPCs)

VARUN, Divya, Gayathri Rajaram SRINIVASAN, Yi-Huan TSAI, Hyun-Je KIM, Joshua CUTTS et. al.

Basic information

Original name

A robust vitronectin-derived peptide for the scalable long-term expansion and neuronal differentiation of human pluripotent stem cell (hPSC)-derived neural progenitor cells (hNPCs)

Authors

VARUN, Divya (840 United States of America), Gayathri Rajaram SRINIVASAN (840 United States of America), Yi-Huan TSAI (840 United States of America), Hyun-Je KIM (840 United States of America), Joshua CUTTS (840 United States of America), Francis PETTY (840 United States of America), Ryan MERKLEY (840 United States of America), Nicholas STEPHANOPOULOS (840 United States of America), Dáša DOLEŽALOVÁ (703 Slovakia, guarantor, belonging to the institution), Martin MARSALA (840 United States of America) and David A. BRAFMAN (840 United States of America)

Edition

Acta Biomaterialia, Oxford, Elsevier BV, 2017, 1742-7061

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30200 3.2 Clinical medicine

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 6.383

RIV identification code

RIV/00216224:14110/17:00095986

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.actbio.2016.10.037

UT WoS

000393247000010

Keywords in English

Human pluripotent stem cells; Human neural progenitor cells; Peptide; Defined conditions

Abstract

V originále

Despite therapeutic advances, neurodegenerative diseases and disorders remain some of the leading causes of mortality and morbidity in the United States. Therefore, cell-based therapies to replace lost or damaged neurons and supporting cells of the central nervous system (CNS) are of great therapeutic interest. To that end, human pluripotent stem cell (hPSC) derived neural progenitor cells (hNPCs) and their neuronal derivatives could provide the cellular ‘raw material’ needed for regenerative medicine therapies for a variety of CNS disorders. In addition, hNPCs derived from patient-specific hPSCs could be used to elucidate the underlying mechanisms of neurodegenerative diseases and identify potential drug candidates. However, the scientific and clinical application of hNPCs requires the development of robust, defined, and scalable substrates for their long-term expansion and neuronal differentiation. In this study, we rationally designed a vitronectin-derived peptide (VDP) that served as an adhesive growth substrate for the long-term expansion of several hNPC lines. Moreover, VDP-coated surfaces allowed for the directed neuronal differentiation of hNPC at levels similar to cells differentiated on traditional extracellular matrix protein-based substrates. Overall, the ability of VDP to support the long-term expansion and directed neuronal differentiation of hNPCs will significantly advance the future translational application of these cells in treating injuries, disorders, and diseases of the CNS.

Citovat

VARUN, Divya, Gayathri Rajaram SRINIVASAN, Yi-Huan TSAI, Hyun-Je KIM, Joshua CUTTS, Francis PETTY, Ryan MERKLEY, Nicholas STEPHANOPOULOS, Dáša DOLEŽALOVÁ, Martin MARSALA and David A. BRAFMAN. A robust vitronectin-derived peptide for the scalable long-term expansion and neuronal differentiation of human pluripotent stem cell (hPSC)-derived neural progenitor cells (hNPCs). Acta Biomaterialia. Oxford: Elsevier BV, 2017, vol. 48, "neuvedeno", p. 120-130. ISSN 1742-7061. Available from: https://dx.doi.org/10.1016/j.actbio.2016.10.037.

@article{1366193,
   author = {Varun, Divya and Srinivasan, Gayathri Rajaram and Tsai, YiandHuan and Kim, HyunandJe and Cutts, Joshua and Petty, Francis and Merkley, Ryan and Stephanopoulos, Nicholas and Doležalová, Dáša and Marsala, Martin and Brafman, David A.},
   article_location = {Oxford},
   article_number = {"neuvedeno"},
   doi = {http://dx.doi.org/10.1016/j.actbio.2016.10.037},
   keywords = {Human pluripotent stem cells; Human neural progenitor cells; Peptide; Defined conditions},
   language = {eng},
   issn = {1742-7061},
   journal = {Acta Biomaterialia},
   title = {A robust vitronectin-derived peptide for the scalable long-term expansion and neuronal differentiation of human pluripotent stem cell (hPSC)-derived neural progenitor cells (hNPCs)},
   volume = {48},
   year = {2017}
}

TY  - JOUR
ID  - 1366193
AU  - Varun, Divya - Srinivasan, Gayathri Rajaram - Tsai, Yi-Huan - Kim, Hyun-Je - Cutts, Joshua - Petty, Francis - Merkley, Ryan - Stephanopoulos, Nicholas - Doležalová, Dáša - Marsala, Martin - Brafman, David A.
PY  - 2017
TI  - A robust vitronectin-derived peptide for the scalable long-term expansion and neuronal differentiation of human pluripotent stem cell (hPSC)-derived neural progenitor cells (hNPCs)
JF  - Acta Biomaterialia
VL  - 48
IS  - "neuvedeno"
SP  - 120-130
EP  - 120-130
PB  - Elsevier BV
SN  - 17427061
KW  - Human pluripotent stem cells
KW  - Human neural progenitor cells
KW  - Peptide
KW  - Defined conditions
N2  - Despite therapeutic advances, neurodegenerative diseases and disorders remain some of the leading causes of mortality and morbidity in the United States. Therefore, cell-based therapies to replace lost or damaged neurons and supporting cells of the central nervous system (CNS) are of great therapeutic interest. To that end, human pluripotent stem cell (hPSC) derived neural progenitor cells (hNPCs) and their neuronal derivatives could provide the cellular ‘raw material’ needed for regenerative medicine therapies for a variety of CNS disorders. In addition, hNPCs derived from patient-specific hPSCs could be used to elucidate the underlying mechanisms of neurodegenerative diseases and identify potential drug candidates. However, the scientific and clinical application of hNPCs requires the development of robust, defined, and scalable substrates for their long-term expansion and neuronal differentiation. In this study, we rationally designed a vitronectin-derived peptide (VDP) that served as an adhesive growth substrate for the long-term expansion of several hNPC lines. Moreover, VDP-coated surfaces allowed for the directed neuronal differentiation of hNPC at levels similar to cells differentiated on traditional extracellular matrix protein-based substrates. Overall, the ability of VDP to support the long-term expansion and directed neuronal differentiation of hNPCs will significantly advance the future translational application of these cells in treating injuries, disorders, and diseases of the CNS.
ER  -

VARUN, Divya, Gayathri Rajaram SRINIVASAN, Yi-Huan TSAI, Hyun-Je KIM, Joshua CUTTS, Francis PETTY, Ryan MERKLEY, Nicholas STEPHANOPOULOS, Dáša DOLEŽALOVÁ, Martin MARSALA and David A. BRAFMAN. A robust vitronectin-derived peptide for the scalable long-term expansion and neuronal differentiation of human pluripotent stem cell (hPSC)-derived neural progenitor cells (hNPCs). \textit{Acta Biomaterialia}. Oxford: Elsevier BV, 2017, vol.~48, ''neuvedeno'', p.~120-130. ISSN~1742-7061. Available from: https://dx.doi.org/10.1016/j.actbio.2016.10.037.

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