PRDM9 interactions with other proteins provide a link between
recombination hotspots and the chromosomal axis in meiosis

J 2017

PRDM9 interactions with other proteins provide a link between recombination hotspots and the chromosomal axis in meiosis

PARVANOV, Emil Damyanov, Hui TIAN, Timothy BILLINGS, Ruth L. SAXL, Catrina SPRUCE et. al.

Basic information

Original name

PRDM9 interactions with other proteins provide a link between recombination hotspots and the chromosomal axis in meiosis

Authors

PARVANOV, Emil Damyanov (100 Bulgaria, belonging to the institution), Hui TIAN (840 United States of America), Timothy BILLINGS (840 United States of America), Ruth L. SAXL (840 United States of America), Catrina SPRUCE (840 United States of America), Rakesh AITHAL (356 India, belonging to the institution), Lumír KREJČÍ (203 Czech Republic, guarantor, belonging to the institution), Kenneth PAIGEN (840 United States of America) and Petko M. PETKOV (840 United States of America)

Edition

Molecular Biology of the Cell, Bethesda, American Society for Cell Biology, 2017, 1059-1524

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.512

RIV identification code

RIV/00216224:14110/17:00094599

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1091/mbc.E16-09-0686

UT WoS

000394268700014

Keywords in English

CXXC FINGER PROTEIN-1; MEIOTIC RECOMBINATION; HISTONE METHYLTRANSFERASES; CHROMATIN MODIFICATIONS; BIOLOGICAL FUNCTIONS; GENE REARRANGEMENTS; ANALYSIS REVEALS; EWING SARCOMA; HOT-SPOTS; METHYLATION

Abstract

V originále

In mammals, meiotic recombination occurs at 1-2 kb genomic regions termed hotspots, whose positions and activities are determined by PRDM9, a DNA-binding histone methyltransferase. We now show that the KRAB domain of PRDM9 forms complexes with additional proteins to allow hotspots to proceed into the next phase of recombination. By a combination of yeast-two hybrid assay, in vitro binding, and co-immunoprecipitation from mouse spermatocytes, we identified four proteins that directly interact with PRDM9's KRAB domain, namely CXXC1, EWSR1, EHMT2, and CDYL. These proteins are co-expressed in spermatocytes at the early stages of meiotic prophase I, the limited period when PRDM9 is expressed. We also detected association of PRDM9-bound complexes with the meiotic cohesin REC8 and the synaptonemal complex proteins SYCP3 and SYCP1. Our results suggest a model in which PRDM9-bound hotspot DNA is brought to the chromosomal axis by the action of these proteins, ensuring the proper chromatin and spatial environment for subsequent recombination events.

Links

GAP207/12/2323, research and development project

Name: Endonuleazová a translokázová aktivita v restričních-modifikáčních komplexéch typu I

Investor: Czech Science Foundation

GA13-26629S, research and development project

Name: SUMO a stability genomu

Investor: Czech Science Foundation

2SGA2773, interní kód MU

Name: Prdm9 - linking the histone modifications and recombination (Acronym: PLHMR)

Investor: South-Moravian Region, Incoming grants

Citovat

PARVANOV, Emil Damyanov, Hui TIAN, Timothy BILLINGS, Ruth L. SAXL, Catrina SPRUCE, Rakesh AITHAL, Lumír KREJČÍ, Kenneth PAIGEN and Petko M. PETKOV. PRDM9 interactions with other proteins provide a link between recombination hotspots and the chromosomal axis in meiosis. Molecular Biology of the Cell. Bethesda: American Society for Cell Biology, 2017, vol. 28, No 3, p. 488-499. ISSN 1059-1524. Available from: https://dx.doi.org/10.1091/mbc.E16-09-0686.

@article{1367105,
   author = {Parvanov, Emil Damyanov and Tian, Hui and Billings, Timothy and Saxl, Ruth L. and Spruce, Catrina and Aithal, Rakesh and Krejčí, Lumír and Paigen, Kenneth and Petkov, Petko M.},
   article_location = {Bethesda},
   article_number = {3},
   doi = {http://dx.doi.org/10.1091/mbc.E16-09-0686},
   keywords = {CXXC FINGER PROTEIN-1; MEIOTIC RECOMBINATION; HISTONE METHYLTRANSFERASES; CHROMATIN MODIFICATIONS; BIOLOGICAL FUNCTIONS; GENE REARRANGEMENTS; ANALYSIS REVEALS; EWING SARCOMA; HOT-SPOTS; METHYLATION},
   language = {eng},
   issn = {1059-1524},
   journal = {Molecular Biology of the Cell},
   title = {PRDM9 interactions with other proteins provide a link between recombination hotspots and the chromosomal axis in meiosis},
   volume = {28},
   year = {2017}
}

TY  - JOUR
ID  - 1367105
AU  - Parvanov, Emil Damyanov - Tian, Hui - Billings, Timothy - Saxl, Ruth L. - Spruce, Catrina - Aithal, Rakesh - Krejčí, Lumír - Paigen, Kenneth - Petkov, Petko M.
PY  - 2017
TI  - PRDM9 interactions with other proteins provide a link between recombination hotspots and the chromosomal axis in meiosis
JF  - Molecular Biology of the Cell
VL  - 28
IS  - 3
SP  - 488-499
EP  - 488-499
PB  - American Society for Cell Biology
SN  - 10591524
KW  - CXXC FINGER PROTEIN-1
KW  - MEIOTIC RECOMBINATION
KW  - HISTONE METHYLTRANSFERASES
KW  - CHROMATIN MODIFICATIONS
KW  - BIOLOGICAL FUNCTIONS
KW  - GENE REARRANGEMENTS
KW  - ANALYSIS REVEALS
KW  - EWING SARCOMA
KW  - HOT-SPOTS
KW  - METHYLATION
N2  - In mammals, meiotic recombination occurs at 1-2 kb genomic regions termed hotspots, whose positions and activities are determined by PRDM9, a DNA-binding histone methyltransferase. We now show that the KRAB domain of PRDM9 forms complexes with additional proteins to allow hotspots to proceed into the next phase of recombination. By a combination of yeast-two hybrid assay, in vitro binding, and co-immunoprecipitation from mouse spermatocytes, we identified four proteins that directly interact with PRDM9's KRAB domain, namely CXXC1, EWSR1, EHMT2, and CDYL. These proteins are co-expressed in spermatocytes at the early stages of meiotic prophase I, the limited period when PRDM9 is expressed. We also detected association of PRDM9-bound complexes with the meiotic cohesin REC8 and the synaptonemal complex proteins SYCP3 and SYCP1. Our results suggest a model in which PRDM9-bound hotspot DNA is brought to the chromosomal axis by the action of these proteins, ensuring the proper chromatin and spatial environment for subsequent recombination events.
ER  -

PARVANOV, Emil Damyanov, Hui TIAN, Timothy BILLINGS, Ruth L. SAXL, Catrina SPRUCE, Rakesh AITHAL, Lumír KREJČÍ, Kenneth PAIGEN and Petko M. PETKOV. PRDM9 interactions with other proteins provide a link between recombination hotspots and the chromosomal axis in meiosis. \textit{Molecular Biology of the Cell}. Bethesda: American Society for Cell Biology, 2017, vol.~28, No~3, p.~488-499. ISSN~1059-1524. Available from: https://dx.doi.org/10.1091/mbc.E16-09-0686.

Detailed Information on Publication Record