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@article{1367105, author = {Parvanov, Emil Damyanov and Tian, Hui and Billings, Timothy and Saxl, Ruth L. and Spruce, Catrina and Aithal, Rakesh and Krejčí, Lumír and Paigen, Kenneth and Petkov, Petko M.}, article_location = {Bethesda}, article_number = {3}, doi = {http://dx.doi.org/10.1091/mbc.E16-09-0686}, keywords = {CXXC FINGER PROTEIN-1; MEIOTIC RECOMBINATION; HISTONE METHYLTRANSFERASES; CHROMATIN MODIFICATIONS; BIOLOGICAL FUNCTIONS; GENE REARRANGEMENTS; ANALYSIS REVEALS; EWING SARCOMA; HOT-SPOTS; METHYLATION}, language = {eng}, issn = {1059-1524}, journal = {Molecular Biology of the Cell}, title = {PRDM9 interactions with other proteins provide a link between recombination hotspots and the chromosomal axis in meiosis}, volume = {28}, year = {2017} }
TY - JOUR ID - 1367105 AU - Parvanov, Emil Damyanov - Tian, Hui - Billings, Timothy - Saxl, Ruth L. - Spruce, Catrina - Aithal, Rakesh - Krejčí, Lumír - Paigen, Kenneth - Petkov, Petko M. PY - 2017 TI - PRDM9 interactions with other proteins provide a link between recombination hotspots and the chromosomal axis in meiosis JF - Molecular Biology of the Cell VL - 28 IS - 3 SP - 488-499 EP - 488-499 PB - American Society for Cell Biology SN - 10591524 KW - CXXC FINGER PROTEIN-1 KW - MEIOTIC RECOMBINATION KW - HISTONE METHYLTRANSFERASES KW - CHROMATIN MODIFICATIONS KW - BIOLOGICAL FUNCTIONS KW - GENE REARRANGEMENTS KW - ANALYSIS REVEALS KW - EWING SARCOMA KW - HOT-SPOTS KW - METHYLATION N2 - In mammals, meiotic recombination occurs at 1-2 kb genomic regions termed hotspots, whose positions and activities are determined by PRDM9, a DNA-binding histone methyltransferase. We now show that the KRAB domain of PRDM9 forms complexes with additional proteins to allow hotspots to proceed into the next phase of recombination. By a combination of yeast-two hybrid assay, in vitro binding, and co-immunoprecipitation from mouse spermatocytes, we identified four proteins that directly interact with PRDM9's KRAB domain, namely CXXC1, EWSR1, EHMT2, and CDYL. These proteins are co-expressed in spermatocytes at the early stages of meiotic prophase I, the limited period when PRDM9 is expressed. We also detected association of PRDM9-bound complexes with the meiotic cohesin REC8 and the synaptonemal complex proteins SYCP3 and SYCP1. Our results suggest a model in which PRDM9-bound hotspot DNA is brought to the chromosomal axis by the action of these proteins, ensuring the proper chromatin and spatial environment for subsequent recombination events. ER -
PARVANOV, Emil Damyanov, Hui TIAN, Timothy BILLINGS, Ruth L. SAXL, Catrina SPRUCE, Rakesh AITHAL, Lumír KREJČÍ, Kenneth PAIGEN and Petko M. PETKOV. PRDM9 interactions with other proteins provide a link between recombination hotspots and the chromosomal axis in meiosis. \textit{Molecular Biology of the Cell}. Bethesda: American Society for Cell Biology, 2017, vol.~28, No~3, p.~488-499. ISSN~1059-1524. Available from: https://dx.doi.org/10.1091/mbc.E16-09-0686.
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