Doxorubicin and Liposomal Doxorubicin Differentially Affect
Expression of miR-208a and let-7g in Rat Ventricles and Atria

J 2017

Doxorubicin and Liposomal Doxorubicin Differentially Affect Expression of miR-208a and let-7g in Rat Ventricles and Atria

NOVÁK, Jan, Jiří ŠÁNA, Tibor STRAČINA, Marie NOVÁKOVÁ, Ondřej SLABÝ et. al.

Basic information

Original name

Doxorubicin and Liposomal Doxorubicin Differentially Affect Expression of miR-208a and let-7g in Rat Ventricles and Atria

Authors

NOVÁK, Jan (203 Czech Republic, guarantor, belonging to the institution), Jiří ŠÁNA (203 Czech Republic, belonging to the institution), Tibor STRAČINA (703 Slovakia, belonging to the institution), Marie NOVÁKOVÁ (203 Czech Republic, belonging to the institution) and Ondřej SLABÝ (203 Czech Republic, belonging to the institution)

Edition

Cardiovascular Toxicology, Totowa, Humana Press, 2017, 1530-7905

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30201 Cardiac and Cardiovascular systems

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.989

RIV identification code

RIV/00216224:14110/17:00096001

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1007/s12012-016-9393-8

UT WoS

000404172500014

Keywords in English

Cardiotoxicity; Doxorubicin; let-7g; lipodox; microRNAs; miR-208a

Abstract

V originále

Anthracyclines use is limited by profound cardiotoxicity. Involvement of miRNAs in anthracycline-induced cardiotoxicity (AIC) is still not completely understood. Thus, the expression of AIC-related microRNAs was determined in rat atria and ventricles after doxorubicin (DOX) and liposomal doxorubicin (L-DOX) administration. Vehiculum, DOX or L-DOX were applied intraperitoneally in a single dose to male Wistar rats (3 groups: control, DOX and L-DOX, respectively). Rats were sacrificed after 24 h, and samples from left atrium (LA)/ventricle (LV) and right atrium (RA)/ventricle (RV) were obtained. Expressions of miR-208a, let-7g and snU6 were determined using qRT-PCR. In the control group, miR-208a was highly abundant in the atria compared to the ventricles and in the left-sided structures compared to the right-sided structures, while let-7g showed only atrio-ventricular gradient with predominant expression in the atria. Administration of both DOX and L-DOX resulted in 38.87 and 23.57% reduction in miR-208a expression in the LV (p = 0.028) and in 13.79 and 14.70% reduction in let-7g expression in the LA (p = 0.015), respectively. Acute administration of DOX/L-DOX alters expression of miR-208a in LV and of let-7g in LA. These changes may partly contribute to the development of AIC.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

MUNI/A/1326/2014, interní kód MU

Name: Kardiovaskulární systém od buňky k lůžku pacienta (Acronym: KASBUNPAC)

Investor: Masaryk University, Category A

MUNI/A/1365/2015, interní kód MU

Name: Kardiovaskulární systém: od modelu přes terapii k prevenci (Acronym: KAMOTEPRE)

Investor: Masaryk University, Category A

Files attached

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Citovat

NOVÁK, Jan, Jiří ŠÁNA, Tibor STRAČINA, Marie NOVÁKOVÁ and Ondřej SLABÝ. Doxorubicin and Liposomal Doxorubicin Differentially Affect Expression of miR-208a and let-7g in Rat Ventricles and Atria. Cardiovascular Toxicology. Totowa: Humana Press, 2017, vol. 17, No 3, p. 355-359. ISSN 1530-7905. Available from: https://dx.doi.org/10.1007/s12012-016-9393-8.

@article{1367107,
   author = {Novák, Jan and Šána, Jiří and Stračina, Tibor and Nováková, Marie and Slabý, Ondřej},
   article_location = {Totowa},
   article_number = {3},
   doi = {http://dx.doi.org/10.1007/s12012-016-9393-8},
   keywords = {Cardiotoxicity; Doxorubicin; let-7g; lipodox; microRNAs; miR-208a},
   language = {eng},
   issn = {1530-7905},
   journal = {Cardiovascular Toxicology},
   title = {Doxorubicin and Liposomal Doxorubicin Differentially Affect Expression of miR-208a and let-7g in Rat Ventricles and Atria},
   volume = {17},
   year = {2017}
}

TY  - JOUR
ID  - 1367107
AU  - Novák, Jan - Šána, Jiří - Stračina, Tibor - Nováková, Marie - Slabý, Ondřej
PY  - 2017
TI  - Doxorubicin and Liposomal Doxorubicin Differentially Affect Expression of miR-208a and let-7g in Rat Ventricles and Atria
JF  - Cardiovascular Toxicology
VL  - 17
IS  - 3
SP  - 355-359
EP  - 355-359
PB  - Humana Press
SN  - 15307905
KW  - Cardiotoxicity
KW  - Doxorubicin
KW  - let-7g
KW  - lipodox
KW  - microRNAs
KW  - miR-208a
N2  - Anthracyclines use is limited by profound cardiotoxicity. Involvement of miRNAs in anthracycline-induced cardiotoxicity (AIC) is still not completely understood. Thus, the expression of AIC-related microRNAs was determined in rat atria and ventricles after doxorubicin (DOX) and liposomal doxorubicin (L-DOX) administration. Vehiculum, DOX or L-DOX were applied intraperitoneally in a single dose to male Wistar rats (3 groups: control, DOX and L-DOX, respectively). Rats were sacrificed after 24 h, and samples from left atrium (LA)/ventricle (LV) and right atrium (RA)/ventricle (RV) were obtained. Expressions of miR-208a, let-7g and snU6 were determined using qRT-PCR. In the control group, miR-208a was highly abundant in the atria compared to the ventricles and in the left-sided structures compared to the right-sided structures, while let-7g showed only atrio-ventricular gradient with predominant expression in the atria. Administration of both DOX and L-DOX resulted in 38.87 and 23.57% reduction in miR-208a expression in the LV (p = 0.028) and in 13.79 and 14.70% reduction in let-7g expression in the LA (p = 0.015), respectively. Acute administration of DOX/L-DOX alters expression of miR-208a in LV and of let-7g in LA. These changes may partly contribute to the development of AIC.
ER  -

NOVÁK, Jan, Jiří ŠÁNA, Tibor STRAČINA, Marie NOVÁKOVÁ and Ondřej SLABÝ. Doxorubicin and Liposomal Doxorubicin Differentially Affect Expression of miR-208a and let-7g in Rat Ventricles and Atria. \textit{Cardiovascular Toxicology}. Totowa: Humana Press, 2017, vol.~17, No~3, p.~355-359. ISSN~1530-7905. Available from: https://dx.doi.org/10.1007/s12012-016-9393-8.

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