a 2016

Microscopic anatomy of monogenean parasites

HODOVÁ, Iveta and Andrea BARDŮNEK VALIGUROVÁ

Basic information

Original name

Microscopic anatomy of monogenean parasites

Authors

HODOVÁ, Iveta (203 Czech Republic, guarantor, belonging to the institution) and Andrea BARDŮNEK VALIGUROVÁ (703 Slovakia, belonging to the institution)

Edition

Mikroskopie 2016, 2016

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/16:00088527

Organization unit

Faculty of Science

Keywords in English

Diplozoidae; confocal laser scanning microscopy; electron microscopy
Změněno: 9/1/2017 13:43, Mgr. Iveta Hodová, Ph.D.

Abstract

V originále

Using a combined approach of confocal and electron microscopy, adults of monogenean parasite Paradiplozoon homoion were investigated for the body organisation and adaptation to the ectoparasitic lifestyle, with emphasis on worm’s forebody and hindbody. Special attention was given to the parasite’s tegument, digestive, reproductive, and excretory systems. Labelling of filamentous actin with fluorescent phalloidin revealed the musculature of the body wall, buccal suckers, the pharynx as well as some parts of reproductive tract. Staining with chloride carmine and counterstaining with DAPI proved to be powerful tools for confocal microscopic detection of various cell types including putative gland cells. Immunofluorescent detection of alfa-tubulin showed to be suitable for visualisation of excretory system and some parts of nervous system.

Links

GAP506/12/1258, research and development project
Name: Interakce hostitel-parazit u krevsajících diplozoidních monogeneí: Výzkum vysoce specializovaných adaptací k parazitismu
Investor: Czech Science Foundation