| BENDL, J., Jan ŠTOURAČ, Eva ŠEBESTOVÁ, Ondřej VÁVRA, Miloš MUSIL, Jan BREZOVSKÝ a Jiří DAMBORSKÝ. HotSpot Wizard 2.0: Automated Design of Site-Specific Mutations and Smart Libraries in Protein Engineering. Nucleic Acids Research. Oxford University Press, roč. 44, W1, s. "W479"-"W487", 9 s. ISSN 0305-1048. doi:10.1093/nar/gkw416. 2016. |
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@article{1367555,
author = {Bendl, J. and Štourač, Jan and Šebestová, Eva and Vávra, Ondřej and Musil, Miloš and Brezovský, Jan and Damborský, Jiří},
article_number = {W1},
doi = {http://dx.doi.org/10.1093/nar/gkw416},
keywords = {MULTIPLE SEQUENCE ALIGNMENTS; FUNCTIONALLY IMPORTANT RESIDUES; FOCUSED DIRECTED EVOLUTION; SATURATION MUTAGENESIS; CORRELATED MUTATIONS; ENZYME PROPERTIES; THERMOSTABILITY; IDENTIFICATION; CONSERVATION; BINDING},
language = {eng},
issn = {0305-1048},
journal = {Nucleic Acids Research},
title = {HotSpot Wizard 2.0: Automated Design of Site-Specific Mutations and Smart Libraries in Protein Engineering},
url = {https://loschmidt.chemi.muni.cz/peg/wp-content/uploads/2016/05/Bendl_2016NAR.pdf},
volume = {44},
year = {2016}
}
TY - JOUR
ID - 1367555
AU - Bendl, J. - Štourač, Jan - Šebestová, Eva - Vávra, Ondřej - Musil, Miloš - Brezovský, Jan - Damborský, Jiří
PY - 2016
TI - HotSpot Wizard 2.0: Automated Design of Site-Specific Mutations and Smart Libraries in Protein Engineering
JF - Nucleic Acids Research
VL - 44
IS - W1
SP - "W479"-"W487"
EP - "W479"-"W487"
PB - Oxford University Press
SN - 03051048
KW - MULTIPLE SEQUENCE ALIGNMENTS
KW - FUNCTIONALLY IMPORTANT RESIDUES
KW - FOCUSED DIRECTED EVOLUTION
KW - SATURATION MUTAGENESIS
KW - CORRELATED MUTATIONS
KW - ENZYME PROPERTIES
KW - THERMOSTABILITY
KW - IDENTIFICATION
KW - CONSERVATION
KW - BINDING
UR - https://loschmidt.chemi.muni.cz/peg/wp-content/uploads/2016/05/Bendl_2016NAR.pdf
L2 - https://loschmidt.chemi.muni.cz/peg/wp-content/uploads/2016/05/Bendl_2016NAR.pdf
N2 - HotSpot Wizard 2.0 is a web server for automated identification of hot spots and design of smart libraries for engineering proteins’ stability, catalytic activity, substrate specificity and enantioselectivity. The server integrates sequence, structural and evolutionary information obtained from 3 databases and 20 computational tools. Users are guided through the processes of selecting hot spots using four different protein engineering strategies and optimizing the resulting library’s size by narrowing down a set of substitutions at individual randomized positions. The only required input is a query protein structure. The results of the calculations are mapped onto the protein’s structure and visualized with a JSmol applet. HotSpot Wizard lists annotated residues suitable for mutagenesis and can automatically design appropriate codons for each implemented strategy. Overall, HotSpot Wizard provides comprehensive annotations of protein structures and assists protein engineers with the rational design of site-specific mutations and focused libraries. It is freely available at http://loschmidt.chemi.muni.cz/hotspotwizard.
ER -
BENDL, J., Jan ŠTOURAČ, Eva ŠEBESTOVÁ, Ondřej VÁVRA, Miloš MUSIL, Jan BREZOVSKÝ a Jiří DAMBORSKÝ. HotSpot Wizard 2.0: Automated Design of Site-Specific Mutations and Smart Libraries in Protein Engineering. \textit{Nucleic Acids Research}. Oxford University Press, roč.~44, W1, s.~''W479''-''W487'', 9 s. ISSN~0305-1048. doi:10.1093/nar/gkw416. 2016.
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