Enzyme Based Test Stripes for the Visual or Photographic
Detection and Quantitation of Gaseous Sulfur Mustard

J 2016

Enzyme Based Test Stripes for the Visual or Photographic Detection and Quantitation of Gaseous Sulfur Mustard

BIDMANOVÁ, Šárka, M.S. STEINER, Martina STEPAN, Kamila VYMAZALOVA, M.A. GRUBER et. al.

Basic information

Original name

Enzyme Based Test Stripes for the Visual or Photographic Detection and Quantitation of Gaseous Sulfur Mustard

Authors

BIDMANOVÁ, Šárka (203 Czech Republic, belonging to the institution), M.S. STEINER (276 Germany), Martina STEPAN (203 Czech Republic), Kamila VYMAZALOVA (203 Czech Republic), M.A. GRUBER (276 Germany), A. DUERKOP (276 Germany), Jiří DAMBORSKÝ (203 Czech Republic, guarantor, belonging to the institution), Zbyněk PROKOP (203 Czech Republic, belonging to the institution) and O.S. WOLFBEIS (276 Germany)

Edition

ANALYTICAL CHEMISTRY, 2016, 0003-2700

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.320

RIV identification code

RIV/00216224:14310/16:00087645

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1021/acs.analchem.6b01272

UT WoS

000377631000069

Keywords in English

PH; OXYGEN; TEMPERATURE; SMARTPHONE; SENSOR

Abstract

V originále

Sulfur mustard is a chemical agent of high military and terroristic significance. No effective antidote does exist, and sulfur mustard can be fairly easily produced in large quantity. Rapid field testing of sulfur mustard is highly desirable. Existing analytical devices for its detection are available but can suffer from low selectivity, laborious sample preparation, and/or the need for complex instrumentation. We describe a new kind of test stripe for rapid detection of gaseous sulfur mustard that is based on its degradation by the enzyme haloalkane dehalogenase that is accompanied by a change of local pH. This change can be detected using pH indicators contained in the stripes whose color changes from blue-green to yellow within 10 min. In addition to visual read-out, we also demonstrate quantitative reflectometric readout by using a conventional digital camera based on red-green-blue data acquisition. Organic haloalkanes, such as 1,2-dichloroethane, have a negligible interfering effect. The visual limit of detection is 20 µg/L, and the one for red-green-blue read-out is as low as 3 µg/L. The assays have good reproducibility +/- 6 % and +/- 2 % for inter-day assays and intra-day assays, respectively. The stripes can be stored for at least 6 months without loss of function. They are disposable and can be produced fairly rapidly and at low costs. Hence, they represent a promising tool for in-field detection of sulfur mustard.

Links

EE2.3.20.0183, research and development project

Name: Centrum experimentální biomedicíny

LM2011028, research and development project

Name: RECETOX ? Národní infrastruktura pro výzkum toxických látek v prostředí

Investor: Ministry of Education, Youth and Sports of the CR

LO1214, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX)

Investor: Ministry of Education, Youth and Sports of the CR

TA04021380, research and development project

Name: Biosenzor pro monitorování toxických látek v životním prostředí

Investor: Technology Agency of the Czech Republic

Citovat

BIDMANOVÁ, Šárka, M.S. STEINER, Martina STEPAN, Kamila VYMAZALOVA, M.A. GRUBER, A. DUERKOP, Jiří DAMBORSKÝ, Zbyněk PROKOP and O.S. WOLFBEIS. Enzyme Based Test Stripes for the Visual or Photographic Detection and Quantitation of Gaseous Sulfur Mustard. ANALYTICAL CHEMISTRY. 2016, vol. 88, No 11, p. 6044-6049. ISSN 0003-2700. Available from: https://dx.doi.org/10.1021/acs.analchem.6b01272.

@article{1367557,
   author = {Bidmanová, Šárka and Steiner, M.S. and Stepan, Martina and Vymazalova, Kamila and Gruber, M.A. and Duerkop, A. and Damborský, Jiří and Prokop, Zbyněk and Wolfbeis, O.S.},
   article_number = {11},
   doi = {http://dx.doi.org/10.1021/acs.analchem.6b01272},
   keywords = {PH; OXYGEN; TEMPERATURE; SMARTPHONE; SENSOR},
   language = {eng},
   issn = {0003-2700},
   journal = {ANALYTICAL CHEMISTRY},
   title = {Enzyme Based Test Stripes for the Visual or Photographic Detection and Quantitation of Gaseous Sulfur Mustard},
   url = {https://loschmidt.chemi.muni.cz/peg/wp-content/uploads/2016/05/Bidmanova_2016AC.pdf},
   volume = {88},
   year = {2016}
}

TY  - JOUR
ID  - 1367557
AU  - Bidmanová, Šárka - Steiner, M.S. - Stepan, Martina - Vymazalova, Kamila - Gruber, M.A. - Duerkop, A. - Damborský, Jiří - Prokop, Zbyněk - Wolfbeis, O.S.
PY  - 2016
TI  - Enzyme Based Test Stripes for the Visual or Photographic Detection and Quantitation of Gaseous Sulfur Mustard
JF  - ANALYTICAL CHEMISTRY
VL  - 88
IS  - 11
SP  - 6044-6049
EP  - 6044-6049
SN  - 00032700
KW  - PH
KW  - OXYGEN
KW  - TEMPERATURE
KW  - SMARTPHONE
KW  - SENSOR
UR  - https://loschmidt.chemi.muni.cz/peg/wp-content/uploads/2016/05/Bidmanova_2016AC.pdf
N2  - Sulfur mustard is a chemical agent of high military and terroristic significance. No effective antidote does exist, and sulfur mustard can be fairly easily produced in large quantity. Rapid field testing of sulfur mustard is highly desirable. Existing analytical devices for its detection are available but can suffer from low selectivity, laborious sample preparation, and/or the need for complex instrumentation. We describe a new kind of test stripe for rapid detection of gaseous sulfur mustard that is based on its degradation by the enzyme haloalkane dehalogenase that is accompanied by a change of local pH. This change can be detected using pH indicators contained in the stripes whose color changes from blue-green to yellow within 10 min. In addition to visual read-out, we also demonstrate quantitative reflectometric readout by using a conventional digital camera based on red-green-blue data acquisition. Organic haloalkanes, such as 1,2-dichloroethane, have a negligible interfering effect. The visual limit of detection is 20 µg/L, and the one for red-green-blue read-out is as low as 3 µg/L. The assays have good reproducibility +/- 6 % and +/- 2 % for inter-day assays and intra-day assays, respectively. The stripes can be stored for at least 6 months without loss of function. They are disposable and can be produced fairly rapidly and at low costs. Hence, they represent a promising tool for in-field detection of sulfur mustard.
ER  -

BIDMANOVÁ, Šárka, M.S. STEINER, Martina STEPAN, Kamila VYMAZALOVA, M.A. GRUBER, A. DUERKOP, Jiří DAMBORSKÝ, Zbyněk PROKOP and O.S. WOLFBEIS. Enzyme Based Test Stripes for the Visual or Photographic Detection and Quantitation of Gaseous Sulfur Mustard. \textit{ANALYTICAL CHEMISTRY}. 2016, vol.~88, No~11, p.~6044-6049. ISSN~0003-2700. Available from: https://dx.doi.org/10.1021/acs.analchem.6b01272.

Detailed Information on Publication Record