PEŠKOVÁ, Marie, Zbynek HEGER, Petr JANDA, Vojtech ADAM and Vladimír PEKAŘÍK. An enzymatic assay based on luciferase Ebola virus-like particles forevaluation of virolytic activity of antimicrobial peptides. Peptides. New York: Elsevier Science Inc., vol. 88, FEB, p. 87-96. ISSN 0196-9781. doi:10.1016/j.peptides.2016.12.015. 2017. |
Other formats:
BibTeX
LaTeX
RIS
@article{1368292, author = {Pešková, Marie and Heger, Zbynek and Janda, Petr and Adam, Vojtech and Pekařík, Vladimír}, article_location = {New York}, article_number = {FEB}, doi = {http://dx.doi.org/10.1016/j.peptides.2016.12.015}, keywords = {Antimicrobial peptides; Cytotoxic peptides; Ebola; Hemolytic activity; Lentivirus; Marburg; Nanoluciferase; Virolytic activity; Virus-like particles}, language = {eng}, issn = {0196-9781}, journal = {Peptides}, title = {An enzymatic assay based on luciferase Ebola virus-like particles forevaluation of virolytic activity of antimicrobial peptides}, volume = {88}, year = {2017} }
TY - JOUR ID - 1368292 AU - Pešková, Marie - Heger, Zbynek - Janda, Petr - Adam, Vojtech - Pekařík, Vladimír PY - 2017 TI - An enzymatic assay based on luciferase Ebola virus-like particles forevaluation of virolytic activity of antimicrobial peptides JF - Peptides VL - 88 IS - FEB SP - 87-96 EP - 87-96 PB - Elsevier Science Inc. SN - 01969781 KW - Antimicrobial peptides KW - Cytotoxic peptides KW - Ebola KW - Hemolytic activity KW - Lentivirus KW - Marburg KW - Nanoluciferase KW - Virolytic activity KW - Virus-like particles N2 - Antimicrobial peptides are currently considered as promising antiviral compounds. Current assays to evaluate the effectivity of peptides against enveloped viruses based on liposomes or hemolysis are encumbered by the artificial nature of liposomes or distinctive membrane composition of used erythrocytes. We propose a novel assay system based on enzymatic Ebola virus-like particles containing sensitive luciferase reporter. The assay was validated with several cationic and anionic peptides and compared with lentivirus inactivation and hemolytic assays. The assay is sensitive and easy to perform in standard biosafety level laboratory with potential for high-throughput screens. The use of virus-like particles in the assay provides a system as closely related to the native viruses as possible eliminating some issues associated with other more artificial set ups. We have identified CAM-W (KWKLWKKIEKWGQGIGAVLKWLTTWL) as a peptide with the greatest antiviral activity against infectious lentiviral vectors and filoviral virus-like particles. ER -
PEŠKOVÁ, Marie, Zbynek HEGER, Petr JANDA, Vojtech ADAM and Vladimír PEKAŘÍK. An enzymatic assay based on luciferase Ebola virus-like particles forevaluation of virolytic activity of antimicrobial peptides. \textit{Peptides}. New York: Elsevier Science Inc., vol.~88, FEB, p.~87-96. ISSN~0196-9781. doi:10.1016/j.peptides.2016.12.015. 2017.
|