PEŠKOVÁ, Marie, Zbynek HEGER, Petr JANDA, Vojtech ADAM and Vladimír PEKAŘÍK. An enzymatic assay based on luciferase Ebola virus-like particles forevaluation of virolytic activity of antimicrobial peptides. Peptides. New York: Elsevier Science Inc., vol. 88, FEB, p. 87-96. ISSN 0196-9781. doi:10.1016/j.peptides.2016.12.015. 2017.
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Basic information
Original name An enzymatic assay based on luciferase Ebola virus-like particles forevaluation of virolytic activity of antimicrobial peptides
Authors PEŠKOVÁ, Marie (203 Czech Republic, belonging to the institution), Zbynek HEGER (203 Czech Republic), Petr JANDA (203 Czech Republic, belonging to the institution), Vojtech ADAM (203 Czech Republic) and Vladimír PEKAŘÍK (203 Czech Republic, guarantor, belonging to the institution).
Edition Peptides, New York, Elsevier Science Inc. 2017, 0196-9781.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10608 Biochemistry and molecular biology
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.851
RIV identification code RIV/00216224:14110/17:00096028
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.1016/j.peptides.2016.12.015
UT WoS 000393930700012
Keywords in English Antimicrobial peptides; Cytotoxic peptides; Ebola; Hemolytic activity; Lentivirus; Marburg; Nanoluciferase; Virolytic activity; Virus-like particles
Tags EL OK, podil
Tags International impact, Reviewed
Changed by Changed by: Soňa Böhmová, učo 232884. Changed: 15/3/2018 17:03.
Abstract
Antimicrobial peptides are currently considered as promising antiviral compounds. Current assays to evaluate the effectivity of peptides against enveloped viruses based on liposomes or hemolysis are encumbered by the artificial nature of liposomes or distinctive membrane composition of used erythrocytes. We propose a novel assay system based on enzymatic Ebola virus-like particles containing sensitive luciferase reporter. The assay was validated with several cationic and anionic peptides and compared with lentivirus inactivation and hemolytic assays. The assay is sensitive and easy to perform in standard biosafety level laboratory with potential for high-throughput screens. The use of virus-like particles in the assay provides a system as closely related to the native viruses as possible eliminating some issues associated with other more artificial set ups. We have identified CAM-W (KWKLWKKIEKWGQGIGAVLKWLTTWL) as a peptide with the greatest antiviral activity against infectious lentiviral vectors and filoviral virus-like particles.
Links
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
MUNI/A/1365/2015, interní kód MUName: Kardiovaskulární systém: od modelu přes terapii k prevenci (Acronym: KAMOTEPRE)
Investor: Masaryk University, Category A
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