An effective clean-up technique for GC/EI-HRMS determination of
developmental neurotoxicants in human breast milk

J 2017

An effective clean-up technique for GC/EI-HRMS determination of developmental neurotoxicants in human breast milk

ČECHOVÁ, Eliška, Marta SEIFERTOVÁ, Petr KUKUČKA, Šimon VOJTA, Ilona QUAAK et. al.

Basic information

Original name

An effective clean-up technique for GC/EI-HRMS determination of developmental neurotoxicants in human breast milk

Authors

ČECHOVÁ, Eliška (203 Czech Republic, belonging to the institution), Marta SEIFERTOVÁ (203 Czech Republic, belonging to the institution), Petr KUKUČKA (203 Czech Republic, belonging to the institution), Šimon VOJTA (203 Czech Republic, belonging to the institution), Ilona QUAAK (528 Netherlands), Marijke DE COCK (528 Netherlands), Margot VAN DE BOR (528 Netherlands) and Anton KOČAN (703 Slovakia, guarantor, belonging to the institution)

Edition

Analytical and Bioanalytical chemistry, HEIDELBERG, SPRINGER HEIDELBERG, 2017, 1618-2642

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.307

RIV identification code

RIV/00216224:14310/17:00096029

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1007/s00216-016-0059-y

UT WoS

000392622800014

Keywords in English

Developmental neurotoxicants; Dialysis; GC-MS; Human breast milk; Non-destructive clean-up

Abstract

V originále

The increasing number of children suffering from developmental disorders has raised questions regarding their association with the presence of environmental contaminants in mothers and children. We therefore developed a new method for the determination of 78 proven and potential developmental neurotoxicants, including polychlorinated biphenyls, legacy pesticides, pyrethroids, and old and new halogenated flame retardants in breast milk. The essential part of sample preparation was dialysis as a non-destructive clean-up step which was newly used at 10 A degrees C and showed more efficient lipid removal (up to 96%) than the conventional methods such as gel permeation chromatography or freezing-lipid filtration and thus ensured low limits of detection (LOD) by reducing the sample volume prior to injection. Next advantages were significant solvent reduction and no risk of sample cross-contamination. Gas chromatography coupled with high resolution mass spectrometry (GC-HRMS) was subsequently used for the separation and compound quantification. The method was validated using breast milk samples fortified with the analyzed compounds. Recoveries for most of the compounds ranged from 63 to 121% with a relative standard deviation of 2-25%, and LODs ranged between 0.001 and 0.87 ng g(-1) lipid weight. The method was applied to breast milk samples from a Dutch birth cohort where 35 out of the 78 compounds were quantified in more than 60% of the samples. For novel flame retardants, the method provides unique results regarding their occurrence in human matrices in Europe. Overall, the analysis of a complex mixture of developmental neurotoxicants could be useful for the assessment of the influence of the studied compounds to child health and development.

Links

CZ.1.07/2.3.00/30.0037, interní kód MU
(CEP code: EE2.3.30.0037)

Name: Zaměstnáním nejlepších mladých vědců k rozvoji mezinárodní spolupráce (Acronym: Postdoc II.)

Investor: Ministry of Education, Youth and Sports of the CR, 2.3 Human resources in research and development

LM2015051, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR

LO1214, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX)

Investor: Ministry of Education, Youth and Sports of the CR

282957, interní kód MU

Name: DENAMIC - Developmental neurotoxicity assessment of mixtures in children (Acronym: DENAMIC)

Investor: European Union, Cooperation

Citovat

ČECHOVÁ, Eliška, Marta SEIFERTOVÁ, Petr KUKUČKA, Šimon VOJTA, Ilona QUAAK, Marijke DE COCK, Margot VAN DE BOR and Anton KOČAN. An effective clean-up technique for GC/EI-HRMS determination of developmental neurotoxicants in human breast milk. Analytical and Bioanalytical chemistry. HEIDELBERG: SPRINGER HEIDELBERG, 2017, vol. 409, No 5, p. 1311-1322. ISSN 1618-2642. Available from: https://dx.doi.org/10.1007/s00216-016-0059-y.

@article{1368293,
   author = {Čechová, Eliška and Seifertová, Marta and Kukučka, Petr and Vojta, Šimon and Quaak, Ilona and de Cock, Marijke and van de Bor, Margot and Kočan, Anton},
   article_location = {HEIDELBERG},
   article_number = {5},
   doi = {http://dx.doi.org/10.1007/s00216-016-0059-y},
   keywords = {Developmental neurotoxicants; Dialysis; GC-MS; Human breast milk; Non-destructive clean-up},
   language = {eng},
   issn = {1618-2642},
   journal = {Analytical and Bioanalytical chemistry},
   title = {An effective clean-up technique for GC/EI-HRMS determination of developmental neurotoxicants in human breast milk},
   url = {http://link.springer.com/article/10.1007%2Fs00216-016-0059-y},
   volume = {409},
   year = {2017}
}

TY  - JOUR
ID  - 1368293
AU  - Čechová, Eliška - Seifertová, Marta - Kukučka, Petr - Vojta, Šimon - Quaak, Ilona - de Cock, Marijke - van de Bor, Margot - Kočan, Anton
PY  - 2017
TI  - An effective clean-up technique for GC/EI-HRMS determination of developmental neurotoxicants in human breast milk
JF  - Analytical and Bioanalytical chemistry
VL  - 409
IS  - 5
SP  - 1311-1322
EP  - 1311-1322
PB  - SPRINGER HEIDELBERG
SN  - 16182642
KW  - Developmental neurotoxicants
KW  - Dialysis
KW  - GC-MS
KW  - Human breast milk
KW  - Non-destructive clean-up
UR  - http://link.springer.com/article/10.1007%2Fs00216-016-0059-y
L2  - http://link.springer.com/article/10.1007%2Fs00216-016-0059-y
N2  - The increasing number of children suffering from developmental disorders has raised questions regarding their association with the presence of environmental contaminants in mothers and children. We therefore developed a new method for the determination of 78 proven and potential developmental neurotoxicants, including polychlorinated biphenyls, legacy pesticides, pyrethroids, and old and new halogenated flame retardants in breast milk. The essential part of sample preparation was dialysis as a non-destructive clean-up step which was newly used at 10 A degrees C and showed more efficient lipid removal (up to 96%) than the conventional methods such as gel permeation chromatography or freezing-lipid filtration and thus ensured low limits of detection (LOD) by reducing the sample volume prior to injection. Next advantages were significant solvent reduction and no risk of sample cross-contamination. Gas chromatography coupled with high resolution mass spectrometry (GC-HRMS) was subsequently used for the separation and compound quantification. The method was validated using breast milk samples fortified with the analyzed compounds. Recoveries for most of the compounds ranged from 63 to 121% with a relative standard deviation of 2-25%, and LODs ranged between 0.001 and 0.87 ng g(-1) lipid weight. The method was applied to breast milk samples from a Dutch birth cohort where 35 out of the 78 compounds were quantified in more than 60% of the samples. For novel flame retardants, the method provides unique results regarding their occurrence in human matrices in Europe. Overall, the analysis of a complex mixture of developmental neurotoxicants could be useful for the assessment of the influence of the studied compounds to child health and development.
ER  -

ČECHOVÁ, Eliška, Marta SEIFERTOVÁ, Petr KUKUČKA, Šimon VOJTA, Ilona QUAAK, Marijke DE COCK, Margot VAN DE BOR and Anton KOČAN. An effective clean-up technique for GC/EI-HRMS determination of developmental neurotoxicants in human breast milk. \textit{Analytical and Bioanalytical chemistry}. HEIDELBERG: SPRINGER HEIDELBERG, 2017, vol.~409, No~5, p.~1311-1322. ISSN~1618-2642. Available from: https://dx.doi.org/10.1007/s00216-016-0059-y.

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