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@article{1368561,
author = {Vereščáková, Hana and Ambrožová, Gabriela and Kubala, Lukáš and Perečko, Tomáš and Koudelka, Adolf and Vašíček, Ondřej and Rudolph, T.K. and Klinke, A. and Woodcock, S.R. and Freeman, B.A. and Pekarová, Michaela},
article_location = {NEW YORK},
article_number = {March},
doi = {http://dx.doi.org/10.1016/j.freeradbiomed.2017.01.003},
keywords = {Nitro-oleic acid; Nitro-fatty acids; Differentiation; Inflammation; Macrophages; Growth factors; Signaling pathways},
language = {eng},
issn = {0891-5849},
journal = {Free Radical Biology and Medicine},
title = {Nitro-oleic acid regulates growth factor-induced differentiation of bone marrow-derived macrophages},
url = {http://www.sciencedirect.com/science/article/pii/S0891584917300047},
volume = {104},
year = {2017}
}
TY - JOUR
ID - 1368561
AU - Vereščáková, Hana - Ambrožová, Gabriela - Kubala, Lukáš - Perečko, Tomáš - Koudelka, Adolf - Vašíček, Ondřej - Rudolph, T.K. - Klinke, A. - Woodcock, S.R. - Freeman, B.A. - Pekarová, Michaela
PY - 2017
TI - Nitro-oleic acid regulates growth factor-induced differentiation of bone marrow-derived macrophages
JF - Free Radical Biology and Medicine
VL - 104
IS - March
SP - 10-19
EP - 10-19
PB - ELSEVIER SCIENCE INC
SN - 08915849
KW - Nitro-oleic acid
KW - Nitro-fatty acids
KW - Differentiation
KW - Inflammation
KW - Macrophages
KW - Growth factors
KW - Signaling pathways
UR - http://www.sciencedirect.com/science/article/pii/S0891584917300047
N2 - Many diseases accompanied by chronic inflammation are connected with dysregulated activation of macrophage subpopulations. Recently, we reported that nitro-fatty acids (NO2-FAs), products of metabolic and inflammatory reactions of nitric oxide and nitrite, modulate macrophage and other immune cell functions. Bone marrow cell suspensions were isolated from mice and supplemented with macrophage colony-stimulating factor (M-CSF) or granulocyte-macrophage colony-stimulating factor (GM-CSF) in combination with NO2-OA for different times. RAW 264.7 macrophages were used for short-term (1-5 min) experiments. We discovered that NO2-OA reduces cell numbers, cell colony formation, and proliferation of macrophages differentiated with colony-stimulating factors (CSFs), all in the absence of toxicity. In a case of GM-CSF-induced bone marrow-derived macrophages (BMMs), NO2-OA acts via downregulation of signal transducer and activator of transcription 5 and extracellular signal-regulated kinase (ERK) activation. In the case of M-CSF-induced BMMs, NO2-OA decreases activation of M-CSFR and activation of related PI3K and ERR. Additionally, NO2-OA also attenuates activation of BMMs. In aggregate, we demonstrate that NO2-OA regulates the process of macrophage differentiation and that NO2-FAs represent a promising therapeutic tool in the treatment of inflammatory pathologies linked with increased accumulation of macrophages in inflamed tissues.
ER -
VEREŠČÁKOVÁ, Hana, Gabriela AMBROŽOVÁ, Lukáš KUBALA, Tomáš PEREČKO, Adolf KOUDELKA, Ondřej VAŠÍČEK, T.K. RUDOLPH, A. KLINKE, S.R. WOODCOCK, B.A. FREEMAN and Michaela PEKAROVÁ. Nitro-oleic acid regulates growth factor-induced differentiation of bone marrow-derived macrophages. \textit{Free Radical Biology and Medicine}. NEW YORK: ELSEVIER SCIENCE INC, 2017, vol.~104, March, p.~10-19. ISSN~0891-5849. Available from: https://dx.doi.org/10.1016/j.freeradbiomed.2017.01.003.
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