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@article{1368569, author = {Klinke, Anna and Möller, Annika and Pekarová, Michaela and Ravekes, Thorben and Friedrichs, Kai and Berlin, Matthias and Scheu, Katrin M and Kubala, Lukáš and Kolářová, Hana and Ambrožová, Gabriela and Schermuly, Ralph T and Woodcock, Steven R and Bruce, and Freeman, and Rosenkranz, Stephan and Baldus, Stephan and Rudolph, Volker and Rudolph, Tanja K}, doi = {http://dx.doi.org/10.1165/rcmb.2013-0063OC}, keywords = {pulmonary arterial hypertension; nitro-fatty acids; inflammation; hypoxia}, issn = {1044-1549}, journal = {American Journal of Respiratory Cell and Molecular Biology}, title = {Protective effects of 10-nitro-oleic acid in a hypoxia-induced murine model of pulmonary hypertension}, url = {http://www.atsjournals.org/doi/abs/10.1165/rcmb.2013-0063OC}, year = {2014} }
TY - JOUR ID - 1368569 AU - Klinke, Anna - Möller, Annika - Pekarová, Michaela - Ravekes, Thorben - Friedrichs, Kai - Berlin, Matthias - Scheu, Katrin M - Kubala, Lukáš - Kolářová, Hana - Ambrožová, Gabriela - Schermuly, Ralph T - Woodcock, Steven R - Bruce, AU - Freeman, AU - Rosenkranz, Stephan - Baldus, Stephan - Rudolph, Volker - Rudolph, Tanja K PY - 2014 TI - Protective effects of 10-nitro-oleic acid in a hypoxia-induced murine model of pulmonary hypertension JF - American Journal of Respiratory Cell and Molecular Biology SN - 10441549 KW - pulmonary arterial hypertension KW - nitro-fatty acids KW - inflammation KW - hypoxia UR - http://www.atsjournals.org/doi/abs/10.1165/rcmb.2013-0063OC N2 - Pulmonary arterial hypertension (PAH) is characterized by adverse remodeling of pulmonary arteries. Although the origin of the disease and its underlying pathophysiology remain incompletely understood, inflammation has been identified as a central mediator of disease progression. Oxidative inflammatory conditions support the formation of electrophilic fatty acid nitroalkene derivatives, which exert potent anti-inflammatory effects. The current study investigated the role of 10-nitro-oleic acid (OA-NO2) in modulating the pathophysiology of PAH in mice. Mice were kept for 28 days under normoxic or hypoxic conditions, and OA-NO2 was infused subcutaneously. Right ventricular systolic pressure (RVPsys) was determined, and right ventricular and lung tissue was analyzed. The effect of OA-NO2 on cultured pulmonary artery smooth muscle cells (PASMCs) and macrophages was also investigated. Changes in RVPsys revealed increased pulmonary hypertension in mice on hypoxia, which was significantly decreased by OA-NO2 administration. Right ventricular hypertrophy and fibrosis were also attenuated by OA-NO2 treatment. The infiltration of macrophages and the generation of reactive oxygen species were elevated in lung tissue of mice on hypoxia and were diminished by OA-NO2 treatment. Moreover, OA-NO2 decreased superoxide production of activated macrophages and PASMCs in vitro. Vascular structural remodeling was also limited by OA-NO2. In support of these findings, proliferation and activation of extracellular signal-regulated kinases 1/2 in cultured PASMCs was less pronounced on application of OA-NO2.Our results show that the oleic acid nitroalkene derivative OA-NO2 attenuates hypoxia-induced pulmonary hypertension in mice. Thus, OA-NO2 represents a potential therapeutic agent for the treatment of PAH. ER -
KLINKE, Anna, Annika MÖLLER, Michaela PEKAROVÁ, Thorben RAVEKES, Kai FRIEDRICHS, Matthias BERLIN, Katrin M SCHEU, Lukáš KUBALA, Hana KOLÁŘOVÁ, Gabriela AMBROŽOVÁ, Ralph T SCHERMULY, Steven R WOODCOCK, BRUCE, FREEMAN, Stephan ROSENKRANZ, Stephan BALDUS, Volker RUDOLPH and Tanja K RUDOLPH. Protective effects of 10-nitro-oleic acid in a hypoxia-induced murine model of pulmonary hypertension. \textit{American Journal of Respiratory Cell and Molecular Biology}. 2014. ISSN~1044-1549. Available from: https://dx.doi.org/10.1165/rcmb.2013-0063OC.
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